1306 Essay

607 words - 3 pages

Sedum sarmentosum Bunge (Crassulaceae) is a species of perennial succulent herb used in folk medicine for the treatment of chronic viral hepatitis in China. Based on our previous research work, this study aims to optimize water extraction technique for total flavonoid isolation from S. sarmentosum and to investigate its protective effect on human hypetocytes. The whole S. sarmentosum plants were collected and water extraction technology was optimized according to orthogonal test with total flavonoid content as evaluation index. Using optimisational technology, the protective effect on human liver cell was investigated. WRL- 68 hepatocytes were incubated with H2O2. The injured hepatocytes induced by H2O2 were assessed through measuring the levels of alanine amino transferase (ALT). Gross flavonoid contents in fresh S. sarmentosum plant were 1.59 mg/ g, higher than 0.97 mg/ g in the dry ones. The best extraction process was as follows: ratio of liquid to material 10:1, temperature 80°C and extracted for 2 h. Water extracts of the plants inhibit the injured hepatocytes induced by H2O2. The optimal dose of fresh plant was 800 mg/ mL while the dry one was 80 mg/ mL. Water extract from S. sarmentosum was able to decrease ALT levels in human blood and exhibit superior hepatoprotective effect. Fresh S. sarmentosum should be preferred over dry plants for the development of healthy flavonoid beverage products.

Key words: total flavonoid, liver cell, Sedum sarmentosum Bunge, Crassulaceae

As a widely distributed wild seasonal vegetable, Shi-Mi is very popular in Southwest China. Its scientific name is Sedum sarmentosum Bunge (Crassulaceae), a species of perennial succulent herb used in medicine to treat chronic viral hepatitis in China and South Korea [1],[2]. According to the Pharmacopoeia of the Peoples Republic of China, S. sarmentosum is not only the source of Sedi herb, but...

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