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A Lab Report On The Investigation Of Temperature On Catalase Activity.

828 words - 3 pages

INVESTIGATION INTO THE EFFECT OF TEMPERATURE ON CATALASE ACTIVITYAIMThe aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalase's different attributes.HYPOTHESISIn this experiment it would be safe to hypothesise that no activity would take place at 1 to 20 degrees. It would be probable that a little activity would take place around 40 degrees. When the water gets to around 60+ the oxygen bubbles start to froth.It is almost certain that this will happen due to the behaviour of the atom. This means that the hotter the water gets the more active the water molecules become. Thus the colder the water gets the less active the water becomes. Another theory is that different things adapt according to their habitat. Therefore, catalase would be most active at around 37 degrees body temperature. This means that in this experiment catalase would be most active in the water bath that is at 40 degrees Celsius.METHODBefore the experiment could be started the following apparatus needed to be obtained:Cork BorerWater Baths at 3,20,40 and 60 degrees CelsiusPotatoRulerStopwatchDetergentHydrogen PeroxideNow that all this apparatus has been obtained, it is possible to start the experiment. Eight test tubes will be required because the experiment is being done twice in each water bath.Use a marker pen to mark the specific temperature on the test tubes so as to make sure that there are no possible errors. Now place these test tubes in a test tube rack. Take the cork borer no. 4, and take a cylinder of potato using the cork borer. Do this until you have eight cylinders. If there are any cylinders that are uneven in size, then take the cylinder again using the cork borer. Now that you have good cylinders, cut them to equal length equal to 1.5cms. cubed. Once this has been done, put each potato cylinder in a test tube. Now it is almost time to start the experiment, so have a stop- watch at the ready. Pour 5cms cubed of hydrogen peroxide (H2O2) into each test tube. Now go to the first water bath that is 3 degrees Celsius and take a pipette and pour 10 drops of detergent into the first 2 test tubes (the test tubes that are labelled 3 degrees Celsius). Once you have done this, immediately start the stopwatch and...

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