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Absorptionsspektrum Der Chlorophylle Essay

2232 words - 9 pages

Protokoll Jens, Schäfer 19.12.2000 1.Thema: Absorptionsspektrum der Chlorophylle 2. Einleitung Mit dem in diesem Protokoll erklärten und durchgeführten Versuch soll zunächst einmal gezeigt werden bei welcher Wellenlänge die Chlorophylle ihre höchste Absorption besitzen. Je mehr licht von der Pflanze, genauer gesagt von den Chlorophyllen absorbiert wird, desto höher ist die Photosyntheserate der Pflanze. Dies bedeute wiederum ein schnelleren Energiegewinn und einen damit verbundenen schnelleren Wachstum der Pflanze.Die Chlorphyle sind ein wichtiger Bestandteil der Pflanzen zu Energiegewinnung. Sie bestehen aus zwei wichtigen teilen: 1. Dem Porphyrinring, welcher ein Magnesiumion enthält. Er ist im wesentlichen für die Absorption des lichtes verantwortlich.2. Eine Phytolkette.Die Chlorophylle a und b unterscheiden sich lediglich an einer chemischen Gruppe am Porphyrinring und einer geringen unterschiedlichen Absorption.In unserem Versuch wird jedoch die Absorption beider Chlorophylle zusammen ermittelt.Die im versuch erhaltenen Messergebnisse zeigen uns in welchem Bereich die Chlorophylle ihr meiste Absorption und somit die Pflanze ihren höchsten Energiegewinn hat.3.Materialien: - Blätter (in unserem Versuch Boretschblätter) - Schere - Erlenmeyerkolben (250 ml) - Heizplatte - A.d.- Scheidetrichter - Methanol - Benzin - Pipette - Quarzküvetten - Spektralphotometer 4.Methodik/Durchführung/Versuchsaufbau: Wir bereiten die zu untersuchende Lösung vor 1.Schritt: Wir zerkleinern, mit eine Schere, 15gr Boretschblätter und trennen sie von den Blattrippen welche wir verwerfen.2.Schritt: Die 15gr Boretschblätter werden in einen Erlenmeyerkolben gegeben und mit 80ml Methanol versetzt.3. Schritt: Anschließend wird der Erlenmeyerkolben in einem Wasserbad 5min erhitzt.Die entstehende grüngefärbte Lösung wird anschließend in einen abgegossen und muss nun im dunkeln aufbewahrt werden.4.Schritt: 20ml dieser methanolischen Lösung werden in einen Scheidetrichter mit 20ml Benzin versetzt.Anschließend geben wir tropfenweise A.d. hinzu bis es zu einer Eintrübung kommt, durch kräftiges schütteln werden nun die Farbstoffe in die Benzinphase überführt. Der wässrige Alkohol enthält Xanthophyll und die Benzinphase Chlorophyll a und b. Karotin wurde bereits bei der Extraktion nicht erfasst.5.Schritt wir füllen nun die Vergleichsküvette mit dem Lösungsmittel (Methanol) und die Messküvette wird mit der Chlorophyll-Lösung gefüllt.6.Schritt: wir erstellen ein Absorptionsspektrum von 400 nm -720 nm und machen alle 5 nm eine Messung. Hierbei müssen wir beachten das nach...

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