Affect Of Chaning P H Essay

3202 words - 13 pages

The effect of changing pH, Temp. and Surface Area on enzymatic activity within the liver

Stage 2 - Biology


Jazz Attwood-McEwan

Summative Practical 1

AbstractThe experiment's aim was to discover the effects that changing the pH, surface area and temperature has on the activity of the enzyme, Catalase found predominately in the liver. The practical also derived the optimum situation in which Catalase would catalyse reactions efficiently and most effectively. The results of the experiment showed that Catalase worked best at a temperature of 30°C, with a pH level of 7 and when the liver was crushed to increase the surface area.IntroductionEnzymes are protein molecules found within living cells. Enzymes catalyse reactions by bringing the substrates together allowing the reaction to occur. Different enzymes are specialised for catalysing different reactions. The enzyme's active site is the corresponding shape to that of the substrate. These reactions occur within cells of living organisms without any changes to the Enzyme. Enzymes work at extremely high speeds being able to catalyse thousands of reactions every second.Hydrogen Peroxide (H₂O₂) is a toxin found in cells that disrupts the activity of the cell. Catalase is the enzyme specialised for breaking down Hydrogen Peroxide. Catalase is a common enzyme and can be found in many cells within living organisms. High concentrations of Catalase can be found in liver cells. Catalase breaks Hydrogen Peroxide into Oxygen and Hydrogen in the following reaction:Hydrogen Peroxide Catalase Water + Oxygen2H₂O₂ Catalase 2H₂O + O₂Enzyme activity can be altered and slowed by changes in temperature, pH and chemical inhibitors. pH changes the shape of the active site lowering the ease at which the substrates can bind with enzyme, which in turn lower the speed at which the enzyme can catalyse reactions. An increase in the temperature causes the denaturing of the enzyme, which makes the enzyme unable to catalyse reactions. A decrease in temperature reduces the speed at which molecules within the cell move this means fewer collision occur between molecules and enzymes, which means a lower reaction rate. Chemical inhibitors on the other hand bind with enzymes and block of the active site making the enzyme unable to catalyse any further reactions.AimTo investigate the effect of changes in the surface area of the liver changes in pH or temperature has on the enzymatic activity of the enzyme Catalase, found in the liver.HypothesisPart A: When the pH of the Hydrogen Peroxide is at 7 the Catalase will catalyse the reaction, Hydrogen Peroxide to Oxygen and water at a faster rate than at a pH level of 3 or 10.Part B: When the liver, which contains high concentrations of Catalase is mixed with Hydrogen Peroxide that has a temperature of 30˚C it will catalyse the reactions at a faster rate than when the Hydrogen Peroxide is at 5˚C or the liver has been...

Find Another Essay On affect of chaning pH

the effect of ph of water on plant growth

809 words - 4 pages affect plant growth including; “soil bacteria, nutrient leaching, nutrient availability, toxic elements, and soil structure” ( Most plants prefer soil to be slightly acidic, ranging from 5.5-7.0. As like most legumes, pisum sativum var. macrocarpon, also known as snow peas, prefer a soil pH range of 6.0-7.0. If the pH of the soil is slightly below neutral around 6.0, then the pea plant #2 will grow faster than the

Effect of pH Buffer on Yeast Reaction

1840 words - 7 pages all around the world can benefit from using a slightly different pH. If my results conclude that a slightly more acidic buffer is a more optimum pH, this also means that people who are making bread dough are using too much quantity of yeast when instead, they could be using a different pH liquid to acquire the bread dough to rise. Hence, I want to investigate how different pH levels affect a yeast reaction.HypothesisI believe that that since

The Affects of Acid Rain on pH and Alkalinity

984 words - 4 pages these nutrients into a river. This would give the aquatic life more nutrients than they need, thus killing them. If the pH is too low, it will affect the solubility of your water pipes, sometimes poisoning your drinking water. The pH range of water that we can drink is between 6.5 and 8.5. For animals, if the pH is too low, it can affect the solubility of metals and nutrients. The range most animals can survive within is between 5 and 9.Many

Introduction to Enzymes

551 words - 2 pages different shapes and functions caused by the order of amino acids, which all control what happens to a molecule by either being present or absent in an anabolic or catabolic reaction, each enzyme catalysing a different reaction. Some examples of enzymes are: Protease, Catalase, Lipase, Maltose and many, many more. There are many factors, which affect the activity rate of an enzyme: Substrate concentration, temperature, pH, Enzyme concentration and

Catalase Lab Report

1701 words - 7 pages this reaction. The rate of oxygen production will help indicate the speed of the reaction. The purpose of this experiment is to find out how temperature, pH, and concentration affect the rate of oxygen production. The experimental hypotheses are: as the catalase concentration increases, the rate of oxygen production will increase, as temperature increases, the rate of oxygen production will increase, and as the pH increases, the rate of oxygen

The Intake of Potassium

1280 words - 6 pages + and inhibited by a decrease in ECF k+ concentration. Other factors that can affect k+ shift in and out of the cell are • ACIDEMIA: increase H+ concentration in acidosis increases potassium concentration in the extracellular fluid. This occurs as a result of exchange of extracellular hydrogen ion for intracellular potassium. • INSULIN: Insulin promote uptake of potassium by skeletal muscle and liver cells. insulin stimulates Na+,k+-ATPase


2018 words - 8 pages Background Most modern biology textbooks will tell you that low-molecular-weight molecules like CO2, NH3 (ammonia), and urea cross cell membranes by simple diffusion. Some scientists postulate, however, that specific pores mediate this transport. A recent study conducted to further this hypothesis examines expression of the water channel aquaporin-1 (AQP-1) and its enhancing affect on CO2 permeation into cells. Why would scientists

What pH the Enzyme Named Peroxidase Works Best At

2588 words - 10 pages What pH the Enzyme Named Peroxidase Works Best At The aim of my investigation is to find out what pH the enzyme named peroxidase works best at. We can find the enzyme peroxidase if we grind up celery, or most other animal and plants. A chemical reaction in plants and animals produce hydrogen peroxide, this can poison them if it is allowed to accumulate. The enzyme peroxidase acts as a catalyst for decomposing hydrogen

Effect of acid rain on Pea growth

3366 words - 13 pages nitrogen and sculpture dioxide by factories. Water vapor in the sky combines with these gases and turns into sulfuric or nitric acid. When it rains they are returned to the main land. Acid rain has a low pH which causes serious damage. Shenzhen is one of the places in China that has an average pH perception around 4.82. Planning This experiment is designed to find out whether the acid water will affect the peas growth or not. The acid I

An Investigation into Determining the Relationship between pH and the Oxidation of Ascorbic Acid

3273 words - 14 pages ) Dehydroascorbic acid (oxidized form) Research Question: This investigation entails answering the ultimate question of how pH values of a solution containing ascorbic acid (C6H8O6) (that have been manipulated by the addition of different volumes of hydrochloric acid) affect the change in the amount of ascorbic acid present in a solution and whether oxidation by atmospheric oxygen has an affect on this. Aim: The aim of this investigation is to

Enzyme Lab Report

3200 words - 13 pages Rivas 3 Testing how environment factors can affect lactose activity was the second part of this experiment. We prepared the following test tubes at different temperatures 4°C, 25°C, and 100°C. For pH on the other hand we had 3 test tubes containing lactase of different buffers of pH 4,7 and 10. We did this to test which test tube would contain the highest concentration of glucose. What we wanted to know was if the lactase would affect the

Similar Essays

Acids And Bases Lab Essay

997 words - 4 pages Acid and Bases LabBy: Ismail MurMs. Iman BukerDate: 10/8/2014Noor Syed, Ridhwaan Anayetullah (Table #3)Purpose: Investigate the acidity of certain substances, and test the buffers of certain acids and bases.Background Research:Buffers: Buffers are important to many things, as they keep a substance's pH steady. They are important to humans, because they keep our blood plasma pH at 7.4 and if it goes too high or too low, that can affect us.Acids

Algae Research Essay

1195 words - 5 pages affect the taste of water, cause water blockages in pipes, produce odour problems, clog filters and affect the productivity of water treatment processes, and cause toxicity in lakes and dams, affecting health of both livestock and humans alike.Aim:The aim of this experiment is to determine whether the pH of water has an effect on the growth rate of algae, and if so, how large the impact is. Throughout the experiment 3 different water filled beakers

Experiment: Effect Of Dissolved Carbon Dioxide On The P H Of Water

1686 words - 7 pages a health risk, but can stain dishes and utensils))4. When Ph of water is low there can be many consequences and effects. ((Even a moderate decrease in pH of water could affect the fish egg hatching success; irritate fish and worst of all, damage membranes. Water with a pH below 4 or higher than 10 is deadly. It will most likely kill majority of sea creatures. Amphibians are at risk when the pH of water is low. If the pH of water is low, heavy

Determining The Influence Of P H On The Activity Of Catalase Enzyme In Potato Tuber Cells

2302 words - 9 pages INTRODUCTIONEnzymes are vitally important for every living organism, because without them the reactions within the cells would occur to slow to sustain life. However there are many factors which affect the rate of enzymes reactions. One of them is pH, which influence on the enzyme activity will be the subject of this investigation. Catalase of potato tuber cells will be the enzyme used in the experiment.RESEARCH QUESTIONWhat is the influence of