This website uses cookies to ensure you have the best experience. Learn more

Effect Of Temperature On Rate Of Reaction Of Catalase

906 words - 4 pages

Effect of Temperature on Rate of Reaction of Catalase

Introduction

This investigation was in an attempt to try to find out how varying
the temperature can affect an enzyme. The enzyme used was catalase
which breaks down Hydrogen Peroxide, this gives off water and oxygen
as effervescence. This effervescence is what is used to measure the
reaction rate of the catalase. The optimal heat for enzyme activity is
proven to be 37oC as anything above this denatures the enzyme.
Denaturing is where the heat energy of breaks down the di-sulphide,
ionic and hydrogen bonds that hold the tertiary structure together,
this in turn changes the shape of the active site and so halts any
reactions that the enzyme would otherwise be able to catalyze.

Method

6 test tubes were set up and 2cm squared of Hydrogen Peroxide was
placed in each tube. Each tube was then placed in a water bath of the
corresponding temperature (20oC, 40oC, 60oC and 80oC), and one in a
beaker of ice (0oC). They were all left in for 10 minutes to take them
all to the desired temperature before the experiment began.6 strips of
potatoes were then cut to 1cm3 using a ruler and scalpel. One of the
strips was put into each tube and then timed with a stop clock and the
level of froth was measured as it rose up the side of the tube every
20 seconds until the froth started to fall. This was done to each tube
individually so that a more accurate measurement was taken.

Results

See Overleaf.

Conclusion

The enzymes were at low activity at around 0oC and then a slight rise
all the way up to 40oC (gentle curve), followed by a steep downward
curve or slow in activity up to 60oC then a very strange rise in
activity at 80oC.

Discussion

As the temperature rose the particles began to vibrate faster, this is
turn caused more collisions between particles. This is why the
reaction rate rose with the temperature, however after a certain point
(40oC), the enzymes were becoming denatured and this caused them to
become useless which is why the reaction slowed to a halt after this
temperature.

This was a poor experiment because:

The potato chips were very hard to get exactly the same. This
...

Find Another Essay On Effect of Temperature on Rate of Reaction of Catalase

To investigate the effect of surface area on the rate of reaction using the enzyme Catalase, found in potato, and the substrate Hydrogen Peroxide

2672 words - 11 pages substrates are bonded to make one product causing an anabolic reaction.There are five factors that effect enzyme activity. They are:·Temperature·pH·Inhibitors·Substrate Concentration·Enzyme ConcentrationThe factor I am changing is surface area of potato containing Catalase. By increasing the surface area of the potato, you make more Catalase molecules be exposed to hydrogen peroxide. This will result in the rate of

To investigate the effect of surface area on the rate of reaction using the enzyme Catalase, found in potato, and the substrate Hydrogen Peroxide

541 words - 2 pages Hydrogen peroxide has an acidic pH which will break down into water which has a neutral pH. This may effect the enzyme action as many enzymes work faster in a neutral environment, the only control over this is the fact that it will happen in each test and to consider its effect at the end of the experiment. I will need to use the same potato and cut a fresh chip at each stage of the experiment as the catalase may become effected by exposure to

How Concentration and Temperature Affects the Rate of Reaction

804 words - 3 pages How Concentration and Temperature Affects the Rate of Reaction between Magnesium Ribbon and Hydrochloric Acid. WHAT I AM INVESTIGATING I am going to investigate how concentration and temperature affects the rate of reaction between magnesium ribbon and hydrochloric acid. MY PREDICTION The thing which I expect to happen is the more acid added to water the faster the reaction will happen. Also the hotter the solution the quicker

The effect of substrate concentrations on the rate of the decomposition of hydrogen peroxide when catalyzed by the enzyme catalase

4245 words - 17 pages For this investigation I have been asked to investigate (by experimentation) the effect of substrate concentrations on the rate of the decomposition of hydrogen peroxide when catalyzed by the enzyme catalase. This is part of our work on the function of enzymes, how they work and the effects of conditions on how they work. We have learnt about the formation of enzyme-substrate complexes, the lock and key model, induced fit model, activation

Investigate the effect of temperature change on the rate of hydrolysis

479 words - 2 pages Investigate the effect of temperature change on the rate of hydrolysis of starch using the enzyme amylase. Amylase investigation --------------------- AIM: To investigate the effect of temperature change on the rate of hydrolysis of starch using the enzyme amylase. These are variables that may affect the rate of the reaction: * concentration of amylase * concentration of iodine * temperature conditions of reaction * pH

To investigate the effect that surface area has on the rate of reaction

1130 words - 5 pages Aim: To investigate the effect of increasing surface area on the rate of reaction.Background information: Hydrogen peroxide is a chemical that consists of hydrogen and oxygen. Its chemical formula is H O . It is a colourless, syrupy liquid that has a sharp odour. It is unstable. Peroxidase is an enzyme which catalyses the reaction. Catalase is an enzyme present in the cells of plants and animals. It is an aerobic (requires oxygen) bacteria. It

The effect of concentration on a reaction rate with hydrolic acid over the magnesium chip

2498 words - 10 pages measuring the reaction this way because I can only calculate the average rate of reaction and not the initial rate.Factors: There are many different factors from which my experiment could be affected by such as the concentration of the acid because the higher the concentrations therefore there are more particles of the reactant present in the same volume.The temperature of the acid also has a greater impact on the reaction rate because if the starting

The effect of concentration of enzyme and enzyme substrate on the rate of an Enzyme-catalysed reaction

3271 words - 13 pages decrease due to denaturation of the enzyme.I would expect from this reaction as the concentration of the enzyme catalase is increased that the amount of oxygen produced would increase, too. This is because catalase speeds up the decomposition of hydrogen peroxide into water and oxygen. Catalase exerts the effect on chemical reactions by providing a place for the hydrogen peroxide, the substrate, to interact. So as the concentration of catalase increases

The Effect Temperature and Concentration Have on a Reaction

2909 words - 12 pages The Effect Temperature and Concentration Have on a Reaction We must produce a piece of coursework investigating the rates of reaction, and the effect different changes have on them. The rate of reaction is the rate of loss of a reactant or the rate of formation of a product during a chemical reaction. It is measured by dividing 1 by the time taken for the

A lab report on the investigation of temperature on catalase activity

828 words - 3 pages INVESTIGATION INTO THE EFFECT OF TEMPERATURE ON CATALASE ACTIVITYAIMThe aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalase's different attributes.HYPOTHESISIn this experiment it would be safe to hypothesise that no activity would take place at 1

The Effect of H2O2 Concentration on Catalase Activity

965 words - 4 pages of meat providing the catalase were added to increasing concentrations of hydrogen peroxide in order to measure the effect of hydrogen peroxide concentrations on the enzyme’s activity. The variable measured was oxygen produced, as water would be too difficult to measure with basic equipment. Research Question What is the effect of the concentration of a hydrogen peroxide solution on catalase activity? Hypothesis The hypothesis is that catalase

Similar Essays

The Effect Of Temperature On The Rate Of Reaction

1175 words - 5 pages The effect of temperature on rates of reaction Aim: The aim of this experiment is to find out if the reaction rate of sodium thiosulphate solution and hydrochloric acid will be affected by a temperature change. Prediction: I predict that as a result of increasing the temperature of the sodium thiosulphate, the rate of reaction will be faster. Because of the increase in temperature, the particles will gain more kinetic energy. This will

The Effect Of Temperature On The Rate Of An Enzyme Catalysed Reaction

1779 words - 7 pages find the effect of temperature on the rate of an enzyme-controlled reaction. Oxygen gas will be produced therefore we can measure the volume of oxygen produced, and compare the rate which is produced at different temperatures to see the effect that changing temperature has on the rate of reaction. I will obtain my results by measuring oxygen evolution in cm3 over various temperatures. If graphs are produced we can measure the gradients of the

Investigating The Effect That Temperature Has On The Rate Of Reaction And Therefore The Amount Of Oxygen Produced

3269 words - 13 pages Decomposition of hydrogen peroxide2H2O O + 2H2OPlanningAim:Our aim is to investigate the effect that temperature has on the rate of reaction and the amount of oxygen that is produced.Prediction:With reference to my theory, I predict that the initial rate of reaction will increase with the temperature rise up to 50°C where the enzyme used and released by the yeast will become denatured and wont function normally, reducing the initial rate of

The Effect Of Temperature On The Rate Of Enzyme Activity

651 words - 3 pages testing the temperature on the rate of enzyme activity is to examine how different temperature might increase or decrease activity. Adding energy in the form of heat to the enzyme will most likely increase activity because more reactions are able to take place. However, cooling down the enzyme and thus removing energy will most likely slow down the process because there is less energy available for the enzymes to utilize for reactions. However