An Experiment To Look At The Effect Of 1 Factor On An Enzyme

1586 words - 6 pages

AimTo design and carry out an experiment to look at one factor which affects the enzyme catalase.Catalase is involved in the breakdown of Hydrogen Peroxide into water and oxygen.2H202 "_ 2H20 + 02It can be found in most cells, however it is mostly concentrated in the liver, kidney and tuber cells of plants (e.g. potato).HypothesisI predict that as the temperature increases the oxygen produced will increase until a point (optimum temperature), then will decrease until no oxygen is produced ( due to denaturation )JustificationAll enzymes have optimum conditions, at which they work best. Therefore in my experiment there will be an optimum temperature i.e. the temperature where oxygen is given off most rapidly.I stated that at a temperature no oxygen would be produced. This is because I believe the enzyme will denature due to the high temperature ( ie the tertiary structure will change ). Due to the denaturation of the enzyme, no oxygen will be produced.I also feel it is important to note that the H2O2 is an unstable compound and at high temperatures (100"aC) it will decompose by itself.ApparatusFor my experiment I will need the following apparatus:"h Conical flask with side on delivery outlet"h Tight fitting bung for the conical flask"h Delivery tube"h Gas-syringe"h Water bath"h Retort stand"h Boss and clampI will set the apparatus up in the following way ¡VControl of variablesFactorControlTemperatureI have chosen to vary this factor using an electronic water bath in my experiment.ConcentrationI will use the substrate from the same bottle without dilution. I will take my cuttings of potato from the same region.Surface AreaI will use the same cork borer and cut all of the potato to the same length. I will also ensure that there is no potato skin present.pHI will again use the substrate from the same bottle, without any attempt to change the pH.It is important to control all of these variables as each of them can affect the results of my experiment.MethodThe method I will use is as follows ¡V"h Set up the apparatus as shown in the diagram"h Put 10 cm3 of H2O2 into the conical flask and allow it to heat to 20"aC."h Add a 3cm long potato tube from the cork borer, ensuring that there is potato skin present. I will then put the bung in place."h Collect the gas that is given off for 2 minutes, timed on a stop watch"h Repeat this procedure heating the H2O2 at temperatures 30"aC, 40"aC, 50"aC, 60"aC, 70"aC and 80"aC, always using the same apparatus."h Repeat the above procedure a total of 3 times to ensure no anomalies have occurred.Justification of methodAs I am investigating the effect of temperature, it is important that the substrate is at the temperature I desire. That is why I will wait for the H2O2 to heat up.I am using 10cm3 because in preliminary work I discovered that this amount of substrate is required to cover the diameter of potato tube I wish to use.Every one of my cuts will be 3cm. This is important as if it is more or less than...

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