Antimicrobial Drug Sensitivity Testing Essay

615 words - 2 pages

     Antimicrobial sensitivity testing is important clinically because the proper selection of an antimicrobial drug in the treatment of a bacterial infection is ideally based on the knowledge of the sensitivities of the infecting organism. In this laboratory exercise you will be working within a group performing a commonly used test that is designed to determine whether or not an isolated organism is able to be treated using a specific antimicrobial drug. The procedure is called sensitivity testing. This testing method allows clinicians to obtain information needed in order to make an informed and concise decision in reference to the antimicrobial drug usage.
Methods and Materials
     Materials being used in this test include:
•     6 Mueller-Hinton agar plates
•     14 cartridges of antimicrobial drugs
•     three automatic dispensers
•     two 1mL pipettes and pipette pump
•     broth culture of Staphylococcus aureus and Escherichia coli
•     spreading rod soaking in ethanol
•     two forceps soaking in ethanol
•     marking pen
•     ruler
•     antimicrobial sensitivity chart
To start off this lab you will
1.     Label the plates with the name of the organisms (three plates per organism)
2.     Create a bacterial lawn by
a.     Inoculate each dish with 0.2mL of the appropriate bacteria. Deposit the inoculum in the center of the plate.
b.     Remove the spreading rod from the ethanol and ignite the ethanol (Do not leave the rod in the Bunsen burner flame longer than is needed to ignite the ethanol.) Allow the ethanol to burn off. Spread the culture over the entire surface of the plate using the sterilized spreading rod. Before continuing wait five minutes to allow the culture to be absorbed by the nutrient agar.
3.     Apply antimicrobial disks to the plate using the dispensers as...

Find Another Essay On Antimicrobial Drug Sensitivity Testing

Antibiotics Essay

1866 words - 7 pages believed that antibiotics interfere with the surface of bacteria cells, causing a change in their ability to reproduce. Testing the action of an antibiotic in the laboratory shows how much exposure to the drug is necessary to halt reproduction or to kill the bacteria. Although a large amount of an antibiotic taken at one time might kill the bacteria causing an illness, such a dose usually would make the person suffer from illness caused by the

Staphylococcus studies. Essay

1438 words - 6 pages were carriers of Staphylococcus, we ran many individual test. These test were to isolate and identify either Staphylococcus aureus or Staphylococcus epidermis and evaluate any possibility of antimicrobial drug resistance. To start off the experiment, I sterile swabbed 2 areas on my body. I swabbed my nose, the most common place for Staphylococcus aureus, and my navel then did a streak plate on Mannitol Salt Agar for each area swabbed. The streak

Antibiotics used with Scaling and Root Planing

1430 words - 6 pages the drug in the periodontal pocket for an extended time, helping the patient fight the bacterial challenge (Wilder,2005). The results of the research will discuss the benefits of minocycline microspheres (Arestin), chlorhexidine gluconate (Perio chip) and doxycycline hyclate gel (Atridox).DiscussionThe PerioChip is a biodegradable gelatin-based polymer system that contains the active antimicrobial, chlorhexidine gluconate (2.5 mg). Each

Molecular-based techniques

2038 words - 9 pages (Washington, J.A., 1996). Polymerase Chain Reaction (PCR) These molecular-based techniques have been revolutionised by the discovery of endonuclease enzymes and the invention of the Polymerase Chain Reaction (PCR) in 1983 by Kary Mullis. This technique allows for the rapid cloning of fragments of DNA so that further testing can be carried out and has simplified the process of nucleic acid amplification in vitro (Bartlett and Stirling, 2003). Using a PCR

Drug Testing

3244 words - 13 pages Drug testing is a laboratory procedure that looks for evidence of drug consumption by analyzing urine, blood, and hair samples. If tested, you must provide a sample in front of an observer to make sure that it is not tampered with. Samples are then sent to a laboratory for analysis, after which the employer is notified of the results (Wodell 1). Exactly who should be subject to the new trend of mandatory drug tests, is the big question being

Nano-microbicides are the Solution to the HIV Pandemic

2851 words - 11 pages viral proteins with the cell membrane generates a mature virus capable of infecting other cells (MALLIPEDDI & ROHAN, 2010). There is need for the development of pharmaceutical formulations that can be conveniently administered to patients and display an acceptable shelf-life and general stability. Challenges in drug delivery are targeting and biocompatibility (DU TOIT et. al., 2009). Microbicides are any compounds or substances that can be

Safe and Effective Pharmaceuticals

867 words - 3 pages (Nageswara at el., 2003). 9. Regulatory aspects To guarantee the quality, clinical performance, efficacy, and safety of a pharmaceutical product, specifications are fixed and approved by the competent regulatory authorities of each country in which the drug is marketed. Analytical testing ensures that these specifications are met by confirming the identity, purity, and potency of drug substances and drug products prior to their release for

Meningitis in the United Kingdom and the United States of America

4159 words - 17 pages urine or serum are undependable (CDC 2011). Serologic testing e.g., enzyme immunoassay for antibodies to polysaccharides can be taken to evaluate, if meningococcal disease is alleged but should not be used to confirm the diagnosis (CDC 2011). CHEMOPROPHYLAXIS In the United States, the main means for avoidance of sporadic meningococcal disease is antimicrobial chemopro-phylaxis of individuals who live in the same household. Close contacts

Biological and Environmental Factors That Affect Cocaine Sensitivity in Macaques

922 words - 4 pages lack of confounding variables. In human drug abusers, the duration of use is often unknown, other drugs such as alcohol and cigarettes may influence results, self-reports may not be accurate and the placebo effect may cause bias. With animal models, there can be repeated testing and the subjects always show up. It is interesting to note that invasive research on apes such as gorillas and chimpanzees has been limited because of ethical concerns

Pharmacokinetic and Pharmacodynamic Interaction of aqueous extract of Cassia auriculata L. leaves and Metformin in rats.

1865 words - 7 pages interpretation of animal data to Humans. Works Cited Ayo R. G, et al. (2004). Antimicrobial activity screening of crude extract from leaves of Cassia nigricans Vahl. ChemClass Journal, 24-26. Berman A.F, etal, (2002). Herb-drug interactions: review and assessment of report reliability. British Journal of Clinical Pharmacology. 52 (5), 587-595. Dey L, etal. (2002). Alternative Therapies for Type 2 Diabetes. Alternative Medicine Review. 7 (1), 45-58

This is a lab report studying the effects of common foods on bacteria. Works cited included.

1987 words - 8 pages Carter, 2002, Investigating Biology, Pearson Education, Inc. as Benjamin Cummings, San Francisco, CA.Purves, William K., David Sadava, Gordon H. Orians, and H. Craig Heller. 2004, Life: The Science of Biology. Courier Companies Inc., Gordonsville, VA.Reese, Alexander R., 1998, Green Tea Combats Drug Resistance, Journal of Antimicrobial Chemotherapy. 42: 211-216.Traynor, Joseph. 2002. Antibiotics in Honey, New Organic Time: Brief Research Reviews. 31: 128-135.

Similar Essays

Antimicrobial Resistance, Resistant Nosocomial Pathogens And Molecular Diagnosis Of Emerging Infectious Diseases

1950 words - 8 pages methods such as, microscopic visualisation, direct culture, sensitivity testing and serological identification’ (Dong et al, 2008). A number of organisms involved in nosocomial infections have been differentiated successfully by antimicrobial susceptibility testing, and treatment therefore administered. Serology is also a useful diagnostics, however not all diseases develop antibodies in the early stage of infection, and thus serology is not

Recent Improvements In Diagnosis And Treatment Of Digestive Infections

1892 words - 8 pages fecal specimens may be used rarely. The culture medium of Brain Heart Infusion Agar base comprising blood, starch, charcoal and bovine serum albumin is used for culture-based tests (43, 45, 61). The culture-based tests are mainly used for sensitivity testing of H.pylori. The antimicrobial susceptibility tests reveal suitable antibiotics for infection treatment and show the pharmacological resistances. An accurate test may lead to a definite and

Bacteria Lab Report

1750 words - 7 pages The Determination of Antibiotic Susceptibility With the Utilization of Bauer-Kirby Disk Fusion MethodIntroduction:The most common way of alleviating the specific symptoms arrived from diseases is to ingest antimicrobial drugs. Chemotherapeutical antimicrobial agents are chemical compounds intended to inhibit or kill rapidly dividing microorganisms. In order to derive an antimicrobial drug, different chemical compounds must be synthetically

Drug Screening Opinion Paper

1026 words - 4 pages AbstractWorkplace Drug Screening procedures and methods vary depending on which substance is sought out by the tester. Several different types of methods for testing an individual are available. Urinalysis testing is the most common technique of screening for substance abuse. Several factors can affect test results. Laws on drug testing have been designed to detect drug use but certain tests can become compromised legally and illegally. Many