This website uses cookies to ensure you have the best experience. Learn more

Antimicrobial Drug Sensitivity Testing Essay

615 words - 2 pages

     Antimicrobial sensitivity testing is important clinically because the proper selection of an antimicrobial drug in the treatment of a bacterial infection is ideally based on the knowledge of the sensitivities of the infecting organism. In this laboratory exercise you will be working within a group performing a commonly used test that is designed to determine whether or not an isolated organism is able to be treated using a specific antimicrobial drug. The procedure is called sensitivity testing. This testing method allows clinicians to obtain information needed in order to make an informed and concise decision in reference to the antimicrobial drug usage.
Methods and Materials
     Materials being used in this test include:
•     6 Mueller-Hinton agar plates
•     14 cartridges of antimicrobial drugs
•     three automatic dispensers
•     two 1mL pipettes and pipette pump
•     broth culture of Staphylococcus aureus and Escherichia coli
•     spreading rod soaking in ethanol
•     two forceps soaking in ethanol
•     marking pen
•     ruler
•     antimicrobial sensitivity chart
To start off this lab you will
1.     Label the plates with the name of the organisms (three plates per organism)
2.     Create a bacterial lawn by
a.     Inoculate each dish with 0.2mL of the appropriate bacteria. Deposit the inoculum in the center of the plate.
b.     Remove the spreading rod from the ethanol and ignite the ethanol (Do not leave the rod in the Bunsen burner flame longer than is needed to ignite the ethanol.) Allow the ethanol to burn off. Spread the culture over the entire surface of the plate using the sterilized spreading rod. Before continuing wait five minutes to allow the culture to be absorbed by the nutrient agar.
3.     Apply antimicrobial disks to the plate using the dispensers as...

Find Another Essay On Antimicrobial Drug Sensitivity Testing

Multidrug TB Essay

1256 words - 6 pages proceed isolated , identified bacteria from the outset . In health care facilities that have isolated conditional TB bacteria should carry out technical antibiotic to know the sensitivity and drug resistance of TB strains , on the basis that helps to treating physician should consult and consider what drugs and drug combinations how to be most effective (eg 3 drug combination is what medicine , time attack how long is acceptable is ... ) . The

Drug Screening Opinion Paper

1026 words - 4 pages AbstractWorkplace Drug Screening procedures and methods vary depending on which substance is sought out by the tester. Several different types of methods for testing an individual are available. Urinalysis testing is the most common technique of screening for substance abuse. Several factors can affect test results. Laws on drug testing have been designed to detect drug use but certain tests can become compromised legally and illegally. Many

Antimicrobial Drug Resistance

1604 words - 7 pages Introduction Antimicrobial drug resistance (AMDR) is a clinical phenomena, it means that the ability of specific microorganism to acquire resistance property against certain antibiotic which it was susceptible before (Meyers, 1987; Russell, 1997). AMDR process is the natural consequence of pathogen adaptation to antimicrobials used in medicine, animal food, agriculture and household activities (Gould & Meer 2005, p. 617). Antibiotic

Multidrug-resistant Streptococcus Pneumoniae

1698 words - 7 pages , et al, 1999; Turett, et al,1999 cited in Whitney, 2000). The main argument that there are dramatic increases in the proportion of pneumococci that are resistant to antibiotics and other antimicrobial agents tested (Whitney, 2000). Despite the high percentage of pneumococcal strains had a sensitivity to some drugs, the multidrug-resistant S. pneumoniae remained cause infections, as a result the situation became worse (Whitney, 2000

Human Pathogengs from Keha and Shinta Rivers

1049 words - 5 pages prescriptions in hospitals are given without clear evidence of infection or adequate medical indication (Silva and Hoffer, 1993). Toxic broad-spectrum antibiotics are sometimes given in place of narrow-spectrum drugs as substitute for culture and sensitivity testing, with the consequent risk of the selection of drug-resistant mutants (Prescott et al., 1999). In Ethiopia drugs are available to the public and thus people may practice self

antibiotic resistance

923 words - 4 pages sensitivity reports, and practicing hand hygiene and standard precautions. Implications for nurse practitioners include reviewing antibiotic therapy with patients, follow prescribing guidelines and antimicrobial formulary for prescribing, and staying abreast new guidelines that may develop to ensure decrease resistance. Swabbing and screening patients suspected of having infections prior to prescribing is essential. Following the Standford guide to

The Pros And Cons Of Employee Drug Testing

1364 words - 6 pages TABLE OF CONTENTS1.DRUG ABUSE IN THE WORKPLACE- THE NUMBERS2.SIGNS OF DRUG ABUSE IN THE WORKPLACE3.CONSEQUENCES OF DRUG ABUSE IN THE WORK-PLACE4.FIVE STEPS TO SET UP ANTI-DRUG PROGRAMS IN COMPANIES5.ASSUMPTION TO ACCOMPLISH EMPLOYEE DRUG TESTING6.THE PROS OF DRUG TESTING ON EMPLOYEES7.THE CONS OF DRUG TESTING ON EMPLOYEES8.SOURCESDrug abuse in the workplace- the numbers1. Drug abuse in the workspace is a popular, global problem if we believe in

Antibiotics

1866 words - 7 pages believed that antibiotics interfere with the surface of bacteria cells, causing a change in their ability to reproduce. Testing the action of an antibiotic in the laboratory shows how much exposure to the drug is necessary to halt reproduction or to kill the bacteria. Although a large amount of an antibiotic taken at one time might kill the bacteria causing an illness, such a dose usually would make the person suffer from illness caused by the

Staphylococcus studies

1438 words - 6 pages were carriers of Staphylococcus, we ran many individual test. These test were to isolate and identify either Staphylococcus aureus or Staphylococcus epidermis and evaluate any possibility of antimicrobial drug resistance. To start off the experiment, I sterile swabbed 2 areas on my body. I swabbed my nose, the most common place for Staphylococcus aureus, and my navel then did a streak plate on Mannitol Salt Agar for each area swabbed. The streak

Antibiotics used with Scaling and Root Planing

1430 words - 6 pages the drug in the periodontal pocket for an extended time, helping the patient fight the bacterial challenge (Wilder,2005). The results of the research will discuss the benefits of minocycline microspheres (Arestin), chlorhexidine gluconate (Perio chip) and doxycycline hyclate gel (Atridox).DiscussionThe PerioChip is a biodegradable gelatin-based polymer system that contains the active antimicrobial, chlorhexidine gluconate (2.5 mg). Each

Molecular-based techniques

2038 words - 9 pages (Washington, J.A., 1996). Polymerase Chain Reaction (PCR) These molecular-based techniques have been revolutionised by the discovery of endonuclease enzymes and the invention of the Polymerase Chain Reaction (PCR) in 1983 by Kary Mullis. This technique allows for the rapid cloning of fragments of DNA so that further testing can be carried out and has simplified the process of nucleic acid amplification in vitro (Bartlett and Stirling, 2003). Using a PCR

Similar Essays

Annotated Bibliography On Antibiotic Essay

1201 words - 5 pages ) Aerobic pathogenic bacteria in post-operative wounds at Moi Teaching and Referral Hospital. East Afr Med J.79:640-644. Anguzu JR, Olila D (2007) Drug sensitivity patterns of bacterial isolates from septic post-operative wounds in a regional referral hospital in Uganda. Afr Health Sci.7:148-54. Bauer AW, Kirby WMM, Sherris JC, Turck M (1966) Antibiotic Susceptibility Testing by a Standardized Single Disk Method. Am J Clin Pathol. 45:493–496

Antimicrobial Resistance, Resistant Nosocomial Pathogens And Molecular Diagnosis Of Emerging Infectious Diseases

1950 words - 8 pages methods such as, microscopic visualisation, direct culture, sensitivity testing and serological identification’ (Dong et al, 2008). A number of organisms involved in nosocomial infections have been differentiated successfully by antimicrobial susceptibility testing, and treatment therefore administered. Serology is also a useful diagnostics, however not all diseases develop antibodies in the early stage of infection, and thus serology is not

Recent Improvements In Diagnosis And Treatment Of Digestive Infections

1892 words - 8 pages fecal specimens may be used rarely. The culture medium of Brain Heart Infusion Agar base comprising blood, starch, charcoal and bovine serum albumin is used for culture-based tests (43, 45, 61). The culture-based tests are mainly used for sensitivity testing of H.pylori. The antimicrobial susceptibility tests reveal suitable antibiotics for infection treatment and show the pharmacological resistances. An accurate test may lead to a definite and

Bacteria Lab Report

1750 words - 7 pages The Determination of Antibiotic Susceptibility With the Utilization of Bauer-Kirby Disk Fusion MethodIntroduction:The most common way of alleviating the specific symptoms arrived from diseases is to ingest antimicrobial drugs. Chemotherapeutical antimicrobial agents are chemical compounds intended to inhibit or kill rapidly dividing microorganisms. In order to derive an antimicrobial drug, different chemical compounds must be synthetically