Biofuels are an importance source of fuel due to the cause that they are infinite and can be found easily compared to fossil fuels. Fossil fuels are finite, hard to find and are not cost-effective as biofuels are. Another good thing about biofuels is that don’t contribute to global warming as much as fossil fuels do. Biofuels are mainly used for as a source of energy and sustainable transport. The ways that these biofuels are produces is that fact they convert living organism’s derivatives into biomass and use it as a fuel source (European Environment Agency, 2011). Then these biomasses can be then turned in chemical, biochemical or thermal conversions (European Environment Agency, 2011).
Alcoholic Fermentation occurs after glycolysis and pyruvate processing (Morton, 1980). During glycolysis, glucose breaks down to form two pyruvate molecules, converting two NAD+ to two NADH, and making two ATP. Then, the two electrons from NADH are transferred to the pyruvate and reduces the molecule. Two carbon dioxide molecules are taken off pyruvate to form two molecules of ethanol. After this, the NAD+ is left and is free to start the whole process over again. The reason why this is a bio fuel is the fact that yeasts derivate is converted into biomass.
In this experiment, three types of yeast were gathered. These yeast were brewing yeast, bread or regular yeast, and ale yeast. Brewing yeast is from a single-celled fungus named Saccharomyces cerevisiae. This type of yeast is used for baking, brewing and winemaking. S. cerevisiae is diploid with high rate of reproduction (Legras et al, 2007). Which means that the carbon dioxide levels should be relatively high when fermentation occurs and would serve as good biofuel.
The age of the yeast cells will depend if the yeast is going to ferment more frequently or not. Aged cells ferment more efficient and a higher rate than virgin or mixed aged cultures (Powell et al, 2003). This would mean that the independent variables would have to age for a while to get the best results of each type yeast. The variables used in the experiment are going to support whether they are going to serve as a good biofuel or not.
During this experiment, the amount carbon dioxide that is given off during the alcoholic fermentation is going to measure to determine the rate of fermentation. The hypothesis is that Ale yeast is going to the highest rate of fermentation than the other two species of yeast. The way to measure which yeast has the highest rate of fermentation is to enclose the yeast with a glucose solution in a syringe with a serological pipette attached to the syringe. To make sure that you get measurements place a water droplet inside the serological pipette to get reading of carbon dioxide produced in a giving time.
After the setup was approved by the teaching assistant, 1.5 mL of regular’s yeast was obtained and mixed with 1.5 mL of glucose solution in a small plastic beaker. The regular yeast in...