Cancer Cells And The Insulin Growth Proteins

1183 words - 5 pages

What if we use what our bodies already produce and use that to combat cancer? Proteins are large molecules that have multifunctional roles in our bodies. They are involved in almost all of our cell functions, like carrying and distributing oxygen throughout our bodies to creating our skin cells. They are the structure to all of our biological cells within all living things. A couple of scientists, from the Chinese University of Hong Kong, conducted several experiments manipulating and creating proteins to inhibit breast cancer cells and colon cancer cells from developing. They modified and reconstruct plasmid DNA which will change the function and information of that specific protein; a few other methods like Northern blotting, Western blotting, Glycosylation staining, and Deglycosylation assay were used to conduct protein and DNA analysis to generate new proteins for the experiment. This experiment will show that insulin-growth proteins have an anti-proliferation effect on cancer cells and can also treat hormone sensitivity.
The scientists have conducted several types of analysis on DNAs and proteins to combine different insulin-growth proteins. IGFBP-3, commonly known as protein-3, is a potent antiproleferative insulin that acts as a blockage of the cell cycle and an induction of apoptosis to the cell. This is a remarkable discovery because a protein that can regulate how much insulin can be transported and it can also signal pathways can effect where the cancer cells are being transported. IGFBP-3 can almost manufacture apoptosis effect on a cancer cell; This can prevent cancer cells from further interfering and development in the human cellular environment. IBFBP-3 is the the abundant protein in its IBFBP family; its function is to transport and stabilize the IGFs in our circulatory system.
A few of the blotting analysis where used in this experiment. Southern blotting is a technique where you extract DNA from your sample. For this experiment, the scientist extracted genomic DNA from a transgenic rice by using the CTAB method; The DNA were digested overnight with a restriction site enzyme called BamHI. After the DNA is separated, they then proceed to transfer the DNA to a nylon membrane where they hybridize and denature the DNA using a probe at 99 degree Celsius. In Northern blotting, RNAs were isolated from the sample seeds and was purified using DNase 1 and RNeasy. The RNA was also transferred to a agarose gel to be separated, then they proceed to hybridize the RNA in a positively charged nylon membrane. Western blotting is a technique where proteins are being identified using antibodies and specifically in this experiment, the proteins are extracted from a dehulled seeds. The proteins were then resolved in 10% Tricine and electrotransfered using a towbin buffer. After the proteins are transferred, using goat anti-body, it was dilution once in polyclonal at 1:1000 dilution and HRP-conjugated at 1:2000 dilution. All of the DNA, RNA, and...

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