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Can Cardiac Disease Medications Demonstrate A Positive Effect On Lung Cells?

824 words - 3 pages

Chronic obstructive pulmonary disease (COPD) is estimated to affect 32 million people in the United States and is the fourth leading cause of death in this country (Kleinschmidt, 2007). The intention of this project was to see if cardiac disease medications can also demonstrate a positive effect on lung cells, such as those in chronic obstructive pulmonary disease. If cardiac disease medications, such as statins, treat COPD effectively then side effects caused from consuming multiple medications simultaneously and prescription expenses could be reduced.
COPD is a lung disease in which the airways that carry air in and out of the lungs are partially obstructed, making it difficult to breathe. In COPD, both the airways and air sacs lose their shape and become floppy (COPD, 2008). COPD develops slowly, and it may be many years before symptoms, such as shortness of breath, are recognized. Cigarette smoking is the most common cause of COPD but breathing in dust and pollution may also contribute to the disease (What Is COPD, 2007). Treatments exist that reduce COPD symptoms but there is no cure. Statins are a class of drugs that lower the level of cholesterol in the blood by reducing the production of cholesterol by the liver (Ogbru, 2007). Statins are currently one of the most widely prescribed drugs as they reduce mortality through both primary and secondary prevention of coronary artery disease (Khurana, 2007). Two of the many types of statins include rosuvastatin (Crestor) and fluvastatin (Lescol). A recent study suggests that statins also slow the lung deterioration that occurs naturally with age. Recent studies have also shown that statins are also potent regulators of inflammation, a major cause of lung deterioration (Park, 2007).
The independent variables that were studied within this research were the statin medications; Crestor and Lescol, the various concentrations of these medications, and the effects of multiple dosing on healthy heart cells and healthy lung cells. The dependant variables were the heart cell and lung cell counts after treatment. Aseptic technique was used throughout experimentation. Lung and heart cells were grown on four well plates. The cells were cultured in their appropriate growth media and after 24 hours of incubation the high, medium, and low treatments were filtered and applied to the cells. The first phase of experimentation determined which concentration was most effective. The cells with the treatments were incubated for 24 hours, and afterwards the cells were counted (Protocols, 2006). The cells were stained with trypan blue, to identify dead cells, and then placed into a hemacytometer chamber, which was then placed under a microscope at 10X magnification to count the number of living cells (Caprette, 2007). The number of live cell in each of the four corner quadrants of the hemacytometer grid were then multiplied by five thousand to...

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