College Lab Report On Enzymes

1306 words - 5 pages

Lab Ex#8: "Enzymes: Catalysts of Life"INTRODUCTIONEnzymes are protein organelles where chemical reactions take place to generate energy within our cells. Without the energy produced from the cell enzyme activity, we would not possess the catalyst activity necessary for energy to produce movement.Each enzyme performs a specific function within our bodies. Those functions performed can be significantly altered with the introduction of variables outside their environment. Variables, such as temperature increases or decreases, can instigate dramatic effects on the enzyme and its intended function.The enzyme has a three-dimensional structure that is specific for the acceptance of a unique like enzyme and when fused together they form a new complex product. The enzyme then returns to its original state, upon which time recycles this process of fusion with its specific substrate.The experiment was to observe what occurs when the enzyme is introduced to a range of temperatures and to determine if an enzyme having similar characteristics will perform the same chemical reaction.PROCEDURE1. Formation and Detection of Benzoquinone:- Stations were set up having three test tubes, a wax marker and a ruler for measuring. We were to indicate measurements on each test tube at 1cm and 2cm from the bottom. We then identified each test tube as 2a, 2b, and 2c.- We filled each test tube with the following: 2a with 1cm of potato extract containing Catechol Oxidase and 1cm of 1% Catechol Solution; 2b with 1cm of potato extract containing Catechol Oxidase and 1cm of dH2O; 2c with 1cm of 1% Catechol Solution and 1cm of dH2O. We tapped each tube to completely mix the solution and recorded the time at zero.- At zero time, each test tube was scaled for color concentration. A color range, zero to five, was to be used. If little color was present, we scaled the tube at zero, and if a deep brownish-gold color was visible, we were to scale the tube at five, all others were scaled within the specified range.- We were also to propose a hypothesis. Our hypothesis was that tube 2a would present the strongest chemical reaction, and that tube 2b and 2c would react, however slightly.- Following a five-minute interval we recorded the color concentration for each tube.2. Effect of pH on Enzyme Activity:- We set up our stations with seven test tubes measuring each test tube at 4cm, 5cm, and 6cm, from the bottom. We identified each test tube, with the numbers 2, 4, 6, 7, 10, and 12, since we felt that the test tubes should correlate with the pH solution being used.- We fill each test tube with the following: 4cm of the pH solutions 2 through 12. We then filled the same test tubes with 1cm of potato extract. A final addition was made of 1cm of 1% Catechol Solution, the substrate for the potato extract enzyme. After completing each chemical additive, we completely mix the solutions and recorded the color concentrations.- The hypothesis was that higher pH solutions would cause a stronger...

Find Another Essay On College lab report on enzymes

Lab report on a fischer esterfication

868 words - 3 pages this radiation and reverse their spin and now oppose the applied magnetic field. The frequency of radiation needed to induce spin conversion is a direct function with the strength of the magnetic field. This amount of energy that is needed to induce the change in spin must have a frequency that matches its angular precessional frequency. This is called a resonance condition and this is what is graphically interpreted on a NMR printout.Objective

Botany Lab Report on Cellular Respiration.

2044 words - 8 pages lost. This would have the same effect on an organism as the electron transport chain not functioning at all.Also during cellular respiration, ATP is released in a series of small steps instead of one large step. This is a more effective method of releasing energy within a cell. There would be great heat released if the reaction occurred in one step and this could kill enzymes as well as the organism. Also, once the reaction occurred, where would

Lab Report: Concentration of Sucrose Affect on Osmosis

1499 words - 6 pages investigating and pour 25cm³ of it into three test tubes and label them test one, two and three. Throw away the remaining 25cm³ of the solution. 4. Place the three test tubes in a test tube rack. 5. Place one potato strip in each test tube and leave in the test tubes for 15minutes. Time the 15minutes using a stopwatch. 6. After 15minutes, remove the potato strips using tweezers and dab them on some tissue to remove excess sucrose solution. 7. Then weigh

Biology Lab Report on the Effects of Photosynthesis

1275 words - 5 pages , Lugol's iodine solution in dropper bottles, 250-mL beakers, 100-mL beakers, tongs, foreceps, Petri dishes, glass-marking pencil, 70% alcoholMethodPART I - Effects Of Light And Dark On Starch FormationThe leaves used for Part I are completely green. A few of these leaves have been entirely and partially covered with black paper two days before the lab. Then the plant was exposed to good light during the day.1. With a glass-marking pencil, label one

Microbiology lab report on Escherichia coli and Staphylococcus aureus.

825 words - 3 pages Microbiology Lab ReportPractica #1BTC307LAmber AmelingmeierThursday, September 18, 2008OBJECTIVESIn this lab experiment two different types of bacteria, Escherichia coli and Staphylococcus aureus, were grown singly and mixed on four different types of agar in order to observe the varying morphologies within the colonies. Resulting data was analyzed to provide understanding of the use of differing culture media and conditions for bacterial

A lab report on the investigation of temperature on catalase activity.

828 words - 3 pages INVESTIGATION INTO THE EFFECT OF TEMPERATURE ON CATALASE ACTIVITYAIMThe aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalase's different attributes.HYPOTHESISIn this experiment it would be safe to hypothesise that no activity would take place at 1

The following is a lab report on fermentation and its inhibitors.

950 words - 4 pages shown.2.) Fermentation results in a excess of NADHAccumulation of NADH causes a problem for anaerobes. They have too much of it and itprevents further oxidation of substrate due to a lack of an NAD+ pool to accept electrons.In many fermentation pathways, the steps after energy generation are performed in part toget rid of the NADH.Pyruvate is a valuable intermediate because it can be used for cell synthesis and manydifferent enzymes can act on it

Lab report on gas chromatograph and the optical activity of carvones.

680 words - 3 pages out is a detector which generates a signal that is recorded on a strip chart recorder.Objective and Materials:The materials for this experiment is listed on page 77-82(Organic Chemistry laboratory Manual, Haden/McNeil, 2003-04)Procedure:The procedure for this experiment is listed on page 77-82(Organic Chemistry laboratory Manual, Haden/McNeil, 2003-04)Data:All data is in laboratory notebookCalculations:The following is a calculation optical

This is a chemistry lab report on an Acid-Base Titration experiment.

2736 words - 11 pages , the pH calculated from the results obtained from the Bromothymol blue experiment displayed only +0.307% error from the pH found with the pH meter.There were several other sources of error or inaccuracies in this lab, however the errors of this lab were relatively minor. There is always error in weighing and measuring due to human and instrument limitations. It was hard to get an exact reading on the pH meter because the numbers fluctuated so

This is a lab report studying the effects of common foods on bacteria. Works cited included.

1987 words - 8 pages Introduction Bacteriology is the study of bacteria, especially as it pertains to medicine (Carter 323). Bacteriology is particularly interested in the effects of certain products, potential antibiotics, on bacteria. The purpose of this experiment was to test the effects of common foods, green tea, honey, and garlic, on bacteria, thus discovering if any of the fore stated products have antibiotic qualities. An antibiotic is any product

A lab report for a high school physics class on the error of measurement.

852 words - 3 pages Purpose:The intention of this lab was to show how inaccurate and imprecise your measurements could be if you did not have the right measuring tools.Procedure1) Tennis Ball DiameterA) circumference methoda) Take a piece of string and wrap it around the tennis ball in as straight of a path as possibleb) Place this string on a ruler and record measurementc) The equation for diameter if a circle isC=pi x dB) average of two methoda) Take two tennis

Similar Essays

Lab Report On Cell Viability

761 words - 3 pages PAGE PAGE 6 Lab Report Lab ReportIntroductionCell viability could be referred as the ability of cell to survive. Number of cells present in tissue could be determined by introducing dyes into the cells in the presence of proper light. This method has its important significance in the field of Biology. There are many fluorescent which are used in laboratories; Calcein is a fluorescent dye which is used widely today. Calcein analysis helps in

Lab Report On Sensory Threshold

1041 words - 4 pages Lab report on Sensory Threshold In this lab the purpose is to test the sensory threshold of a human body. To say it in terms that are easier to understand, we are testing how accurate certain points are on a body with response to touch. We are using three points on the body, the forearm, palm, and index finger. With these three points we will be testing to see how sensitive each point on the body is by measuring the distance of which we can feel

Lab Report On Serial Communication And Accessing Ibm Comm. Ports

1113 words - 4 pages Lab Report on Serial Communication and Accessing IBM Comm. Ports Lab Title: Serial Communication and accessing IBM comm. ports using DOS and BIOS 1. One of the main differences between serial and parallel communication is the number of wires used to

Lab Report On Measuring The Rate Of Conversion Of Hydrogen Peroxide Using Enzyme Catalysis

866 words - 3 pages Lab Report on Measuring the Rate of Conversion of Hydrogen Peroxide using Enzyme Catalysis In essence, the main objective was to use chemical titration to measure and then calculate the rate of conversion of hydrogen peroxide (H2O2) to water and oxygen by using the enzyme catalase. Other purposes of the lab were; to measure the effects of changes of temperature, pH, enzymes concentration, and substrate concentration on rates of an enzyme. The