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Comparing Amount Of Glucose In Orange Fruit, Grapefruit And Lemon Fruit

2176 words - 9 pages

Comparing Amount of Glucose in Orange Fruit, Grapefruit and Lemon Fruit

I have been given 4% of glucose solution, benedict's solution and
distilled water to find out the concentration of glucose of these
three juices; orange, lemon, and grapefruit.

The method of serial dilution has to be done to work out the
concentration; this is because the concentration of glucose is far too
concentrated. A dilution factor has to be worked out; I will be using
a dilution factor of 5, which means the amount of glucose and the
percentage of glucose will always divide by 2 as the dilution solution
carries on.


Orange, lemon and grapefruit are all fruits which contain
carbohydrates which include reducing sugars. Glucose is a hexose
sugar, which is a monosaccharide with six carbon atoms in each of its

Benedict's solution is a deep-blue alkaline solution used to test for
the presence of the aldehyde functional group, CHO - which are present
in reducing sugars. Benedict's solution changes colour when a reducing
sugar is present because of a chemical reaction, which reduces the
Copper II sulphate (which is soluble) to copper I oxide, which is
insoluble and produces a precipitate. The benedict's solution changes
colour from blue to green, yellow, orange, brown and finally red - as
the amount of sugar increases.


Instruments for the analysis of coloured solutions include the
colorimeter. In both these instruments, light emitted from a source
passes through the sample to be analysed, and the intensity of the
emergent beam is measured by a photoelectric device and recorded on a
milliameter. The wavelength of the light incident on the test solution
must be controlled: in the colorimeter this is done by placing a
coloured filter in the path of the incident light.


I predict that orange juice is the most concentrated out of the three
juices, which are orange, grapefruit and lemon. Orange juice should
have a higher concentration of glucose, followed by Grapefruit then
lemon. I have predicted this because of a source I have obtained which
showed that orange has the greatest number of grams of

The juice sample

The amount of monosaccharides in grams







(Manuel of nutrition by Mafa)

If there is a greater number of monosaccharides there would be a
higher concentration of glucose present. Benedict's test can prove
this as the more reducing sugar present the greater the amount and the
darker the colour of the precipitate.

A Benedict's test is a test, which can be used to show the presence of
reducing sugars.

I predict that orange juice would have the a more red precipitate
colour solution followed by grapefruit with a slight brownish...

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