This website uses cookies to ensure you have the best experience. Learn more

Confirmation Of Bacterial Transformation And Dna As The Inducing Material

1204 words - 5 pages

Confirmation of Bacterial Transformation and DNA as the Inducing Material
Biol2000Lab5: Randall Barley
April 15 2014 (late)
Julius Moore

Confirmation of Transformation and DNA as the Inducing Material
The experiments in this report are a recreation the famous Griffith and Avery experiments which discovered the transformation process that bacteria can undertake and that DNA is the genetic material, respectively (Griffth, 1928. Avery 1944). Bacteria can incorporate foreign DNA found in their medium into their genomes in a process known as transformation. This process can be accelerated in CaCl solution at colder temperatures. The first part of this report deals with confirming that transformation does occur following a standard procedure, and the second of the report uses a similar procedure to confirm that DNA is the particle that induces transformation.
In the following procedures Escherichia coli was used:
(Part 1) For the first set of experiments a solution of half live ampicillin sensitive strain and half of a heat killed ampicillin resistant strain was mixed and incubated at 37°C for forty five minutes. The resulting fluid was spread onto a prewarmed LB and a prewarmed LB Amp100 medium, respectively. In addition six controls were set up which shared a LB and a LB Amp100 medium. These controls were given their own area on the media and streak plated. The controls consisted of the live ampicillin sensitive strain, the dead ampicillin resistant strain and the live ampicillin resistant strain plated onto each medium. Aseptic technique was used in treating all solutions, media and transfers between.
(Part 2) In the second set of experiments 1.0µL of kanamycin sensitive bacteria was added to two test tubes, both subjected to the same treatment. The bacteria in the tubes were then pelleted by centrifugation at 3000rpm for seven minutes. Most of the supernatant was discarded and the pellet suspended in 250µL of prechilled CaCl2 solution. Next the bacteria were repelleted by centrifugation at 2000rpm for two minutes after which the supernatant was discarded and the pellets resuspended in 100µL of chilled CaCl2 solution. The 100µL of now competent in each tube were combined, and then 50µL of the 200µL was placed into three separate tubes.
Sets of controls and treatments of various hydrolysing enzymes were prepared according to Table 3. The experimental treatments had 1µL of cell extract added, namely blenderized heat-killed ampicillin resistant cells, 1µL of an enzyme added and 3µL of buffer. The first controls contained 1µL of cell extract and 4µL of buffer. The second controls had 5µL of buffer prepared. These mixtures were incubated for fifteen minutes at 37°C, then incubated at 65°C for ten minutes. 0.5µL of 25mM EDTA was added to the DNAse and RNAse treatments prior to the ten minute incubation.
The 5µL mixtures were mixed with their own 50µL of competent cells then incubated on ice for ten minutes. Next...

Find Another Essay On Confirmation of Bacterial Transformation and DNA as the Inducing Material

The pros and cons of DNA profiling

875 words - 4 pages DNA profiling in matching to people depends on the technique used and particularly the number of loci checked. While this is getting better so the chances of a false match are very small, the courts have not yet accepted it as absolute proof of identity. If a particular exhibit is handled by a number of people the DNA profiling results indicate a mixture; so interpretation is not always straightforward. Also how a person process's DNA samples

Chemistry and the Structure of DNA

690 words - 3 pages Genetics relies on chemistry to explain phenomena related to the field. The structure of DNA relies on chemistry. In fact, when James Watson and Francis Crick discovered the structure of DNA, they did so by building models based on the laws of chemistry. Chemistry also relates heavily to the structure and function of one of the main products of DNA: protein. Chemistry dictates the structure of DNA. DNA is a polymer of monomers called nucleic

Traditional Sacrifices and Sacrifices of the Material

1025 words - 5 pages villagers to get home for noon dinner” (Kennedy and Gioia, 2013, pp. 251). The villagers are portrayed as normal people gathering, extending their hands in friendly and warm communication. Tessie Hutchinson shares and enjoys this ritual event. It was only when she “won” the lottery that she developed bias and judgment. Tessie protests the process of the lottery and the town’s methods, “It isn’t fair, it isn’t right” Mrs. Hutchinson screamed

The Transformation of Immigrant Parents and Children

2065 words - 8 pages idea of changing your culture and morals is considered a very rebellious act within families. It is almost shameful to change your values. It is considered as going against the family and their beliefs. Therefore it is very problematic if a youth believes in a different lifestyle than the rest of the family or their original lifestyle. When generally analyzing immigrant’s movement process, despite their race, all immigrants have a common

The History and Transformation of Korea

2176 words - 9 pages , Baekje, and Silla, the spread of the Chinese influence (Sinicization), and the formation of alliances between the East Asian nations such as the Tang dynasty of China allied with Silla, and the Yamato kingdom of Japan allied with Baekje changed the people’s perception of Korea as a nation because of the influence of different emerging cultures. Religion is also one of the main contributors that made Korea a subject for change because it allowed the

The Transformation of Music: Radio and Composition

1440 words - 6 pages Goldstein 107). Not unsurprisingly, he preferred his new career (Edmund and Goldstein 107). Moments such as those have fortified the ties of music and reality throughout time. Nevertheless, music has been a definite part of culture since the earliest ages. It has also been altered on several occasions in a variety of ways. One defining period of change came during the 1930s, when the United States and the rest of the world saw a significant decrease

The DNA of Relationships

849 words - 4 pages that would mean no losers in teamwork? The second question is important to me because I never had the thought of having a policy that would keep the relationships together when it requires teamwork. As shown above, The DNA of Relationships was a powerful book to read for the class. I believe of that the actions to endanger all my relationships and taking the responsibility to have safe, full of heart steps to face the dangers with the help of God. From the book, I now have the full picture of what it takes to keep the relationships strong and safe because not all people are the same around me and I must take the right steps to protect all my relationships from harm.

Coevolution of Bacterial Gut Microbiota and the Human Adaptive Immune System

1185 words - 5 pages Within the gastrointestinal tract of the human body thrive trillions of bacteria, comprising what is known as the microbiota (Slack et al. 2009, Figure 1). The microbiota can be defined as the combination of microorganisms living simultaneously in a location, with that location being the human body (Round et al. 2009). Many years of evolution have contributed to the relationship that is observed between human intestinal bacteria and the

The Transformation of Othello

693 words - 3 pages TRANSFORMATION OF OTHELLOIn Shakespeare's play Othello the transformation of the main character Othello "the moor", both in terms of attitudes towards life and the state of mind he is in, is the main axis by which various types of other contrasts are worked up. In this play readers are ended up with a final where a respectful and strong general turned in to a weak person that is manipulated by the vicious plans of Iago, the "evil" character of

The Transformation of America

1347 words - 5 pages 2001, the United States seemed to be thrust into a new world of Weapons of Mass Destruction, Jihadists, and a global fight against terrorism; bombs were dropped, ground forces were deployed in foreign states, and anyone who publicly questioned the urgency of war was at risk to be labeled a traitor. This one event was indelibly branded on the consciousness of the world and if often seen as a moment of sudden transformation. Most Americans believe

The Transformation of Gdansk

826 words - 3 pages The city of Gdańsk has played an important role in the transformation of Europe. But the same thing that today makes us proud about our past, could very well be what makes us a monument in the future. A legacy enacted solely through commemoration does not constitute a creative starting-point for adapting to contemporary challenges. Gdańskian, Polish, or European. Gdańsk needs to transform. And Europe will play an important role in the

Similar Essays

Five Pillars Of Islam And The Confirmation Of The Ten Commandments In The Quran

4691 words - 19 pages wife1010109You shall not covet anything that belongs to your neighbor10Islam's confirmation of the Ten Commandments:Many might believe that Islam and Christianity are totally different in their teachings. This might be true when it comes to certain practices such as drinking alcohol or eating pork for example, yet some of the core teachings of both religions are similar. The same prophets appear in both the Quran as well as in the Bible, maybe under Arabic

The Ancient Face: The Greek Theatrical Mask As A Symbol Of Performance And Transformation

1259 words - 6 pages of dramatic portrayal. This less-corporeal function positioned the mask not only as a symbol of the performing arts but also one of a transformational process that provided access to the divine. This symbolic value has evolved through the ages and remains significant today as a symbol of dramatic and metaphorical transformation. Because they were fashioned of short-lived materials such as linen, animal glue, and plaster , no examples of the

The Transformation Of Foil Characters: An Comparison Of Hal And Hotspur As Foil Characters

910 words - 4 pages In Henry IV Part 1, by William Shakespeare, Shakespeare intends the readers to view Hal and Hotspur as foil characters. A foil character is a character who contrasts with another character, usually to highlight one of their attributes (“Foil.”). Hotspur, a well renowned war hero, is respected among many people in his society. One of those people includes King Henry IV. King Henry IV is ashamed of his own son and wants him to be more like Hotspur

The Collection And Retention Of Dna

2170 words - 9 pages Introduction DNA testing has been the center of attention in many criminal justice cases. The United States corrections centers have utilized the DNA testing process. Seventeen death row inmates have been exonerated by the use of these tests. Earl Washington was convicted of rape and murder in 1984. Although he confessed to the rape, he was also diagnosed as being mentally retarded. In October of 2000 Mr., Washington was given a DNA test