Coursework Osmosis Essay

1092 words - 4 pages

Coursework - OsmosisPlanningAim: To investigate what concentration of sucrose solution is isotonic to potato cell sap.Apparatus:0M sucrose (aq) (distilled water)2M sucrose (aq)PotatoChip cutterKnifeBoiling tubesMeasuring cylinder (25cm3)Vernier callipers (to nearest 0.1mm)Balance (to nearest 0.01gram)Safety: I will ensure safety around the lab by: wearing safety goggles, laying paper towels, removing baggage from walking space, push stools under the bench, stand up, walk carefully and slowly - don't rush and handle equipment and apparatus carefully.Preliminary Experiment: I did a small experiment before doing the real one so I could find a suitable range to base my investigation round. I used 3 chips in 3 boiling tubes, each containing 20cm3 of: 0M sucrose, 0.5M sucrose and 1M sucrose.Moles of Sucrose /M Original Mass /g Mass after 3 hours /g Up/Down in mass0M 8.02 9.94 Up0.5M 8.18 7.83 Down1M 8.83 7.20 DownAs you can see from my preliminary results, I should investigate between 0M sucrose and 0.5M sucrose as a suitable range.I predicted that the isotonic concentration lies between 0.35M and 0.4M.Method:I will cut 5 chips with no skin on them and have them approximately the same length, width, breadth and mass (using the balance and Vernier callipers for extra accuracy), then I will dry off the sap completely. I will use the measuring cylinder to accurately make: 20cm3 0.1M sucrose, 20cm3 0.2M sucrose, 20cm3 0.3M sucrose, 20cm3 0.4M sucrose and 20cm3 0.5M sucrose, each putting it into a boiling tube. I will then place the 5 identical chips in each boiling tube and leave for 3 hours so osmosis can occur fully.Scientific Knowledge: A potato's cell has a fully permeable cell wall, but a selectively permeable cell membrane, which allows certain substances to enter and not enter the cell, such as water and sucrose in this instance. In all things, everything moves from high concentration areas to low concentration areas, when in water, this process is called 'Osmosis'. With potato (plant) cells, it swells up as water enters via osmosis, however, it won't burst because the cell wall is tough, and it only stretches out slightly. When it can't stretch anymore, it is called 'Tugor'; this is what makes the plant firm and stand up. The opposite, when the cell membrane shrinks to its limit...

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