Determining The Influence Of P H On The Activity Of Catalase Enzyme In Potato Tuber Cells

2302 words - 9 pages

INTRODUCTIONEnzymes are vitally important for every living organism, because without them the reactions within the cells would occur to slow to sustain life. However there are many factors which affect the rate of enzymes reactions. One of them is pH, which influence on the enzyme activity will be the subject of this investigation. Catalase of potato tuber cells will be the enzyme used in the experiment.RESEARCH QUESTIONWhat is the influence of different values of pH on the activity of catalase in potato tuber cells?VARIABLESIndependent: potato discs treatment (placing them into buffer solutions of different pH values, mixed with hydrogen peroxide)Dependent: the activity of the catalase, reflected by the rate at which oxygen is evolved, measured at every time the pH is changed.Controlled: whether the potato discs were chemically threaten, whether the catalase and hydrogen peroxide concentration was the same in every sample ( whether the potato discs were accurately cut and whether they all came from the same potato, whether the same fresh hydrogen peroxide was used in all samples) and whether the temperature remained the same during the whole experiment.HYPOTHESISDifferent pH values influence the activity of catalase. It was obtained from the literature, that the range of pH values, at which catalase works is approximately 4 to 9, with an optimum pH of 7. Bearing this in mind the following hypothesis has been made:If the potato discs are placed into a test tube with hydrogen peroxide and a buffer solution at pH approximately 7, then the activity of catalase will be the greatest. If the pH value is less or more than 7 the activity of the enzyme will be smaller.THEORYAn enzyme is a biological catalyst. This means that it's a substance, produced in living organisms, which speeds up a certain reaction, because it lowers the activation energy. Activation energy is the amount of energy, which is needed for the reaction to start.Catalase is one of the fastest acting enzymes known, which speeds up the decomposition of hydrogen peroxide, liberating oxygen gas and releasing energy as shown below:2H2O2 2H2O + O2Hydrogen peroxide is a toxic by-product of metabolism in certain pant and animal cells, and in a reaction catalyzed by catalase, it is efficiently decomposed into harmless substances- water and oxygen.Buffer solution is an aqueous solution consisting of a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid. It has the property that the pH of the solution changes very little when a small amount of acid or base is added to it. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications.A pH is the measure of the acidity or alkalinity of a solution (see figure 1). An acidic solution has many hydrogen ions (H+) and a pH below 7. An alkaline, or basic, solution has very few hydrogen ions and a pH above 7. A neutral solution has a pH of 7.The optimum pH of an...

Find Another Essay On Determining the Influence of pH on the Activity of Catalase Enzyme in Potato Tuber Cells

To find out the optimum pH at which the enzyme catalase would best break down hydrogen peroxide.

2844 words - 11 pages catalase for the investigation. The four choices of sources which were available were liver, apple, yeast and potato. Each source was cut up into 1cm3 sections and placed in four separate test tubes which each contained 5ml of H2O2. Observations were made on the physical evidence of what happened.Catalase source Degree of bubbling Number of timesrelights glowing splintpotato normal 1apple little 0liver a lot 7yeast Quite a lot 3As the result show


651 words - 3 pages heat can cause enzymes to denature and stop functioning. The tolerance of different pH levels for enzymes varies. The majority of enzymes function best at a neutral pH between 6 and 8. Some enzymes are able to work in very acidic environments though, and other in more basic ones. Salinity, or the amount of salt present, is also a major influence of enzyme activity. Not enough salt changes the shape of functions due to the incredibly strong

What affect the substrate has on the rate of reaction with the enzyme in potato?

946 words - 4 pages Enzyme InvestigationTaskTo find out what affect the substrate has on the rate of reaction with the enzyme in potato.Variables1. PH2. Temperature3. Concentration of substrate4. Concentration of enzyme5. Surface area of potato lumps6. TimeKey VariablesInput variables - concentration of substrateOutput variable - volume of gasPredictionI think that to start with, the higher the concentration of the

Effects of Catalase Type on Enzyme Action

912 words - 4 pages Enzyme Action:Testing Effect of Type Catalase on Catalase ActivityIsabelle KnezevicMr. CottinghamNovember 11, 2013IB Biology Period 7Introduction:Enzymes are responsible for most of the chemical activities of living organisms. Enzymes are globular proteins with specific 3D formations that act as catalysts, or substances that speed up chemical reaction. All living organisms use enzymes to speed up various reactions. H2O2 is a substance that is

A lab report on the investigation of temperature on catalase activity.

828 words - 3 pages INVESTIGATION INTO THE EFFECT OF TEMPERATURE ON CATALASE ACTIVITYAIMThe aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalase's different attributes.HYPOTHESISIn this experiment it would be safe to hypothesise that no activity would take place at 1

The effect of substrate concentrations on the rate of the decomposition of hydrogen peroxide when catalyzed by the enzyme catalase.

4245 words - 17 pages energies of normal reactions and enzyme-catalyzed reactions, equilibrium, specificity and denaturation.Let me write specifically about the enzyme, catalase and the substrate, hydrogen peroxide. In organisms, hydrogen peroxide is a toxic by-product of metabolism, of certain cell oxidations to be more specific. Hydrogen peroxide on its own is relatively stable and each molecule can stay in this state for a good few years. Its decomposition therefore

Enzyme Kinetics Lab Report: The reaction rate of enzyme, '-amylase in Starch-Iodine solution at different temperatures and pH levels

1996 words - 8 pages Abstract:This experimentation was to evaluate absorbance and the reaction rate of an enzyme, '-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures, and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with

Investigate the Water Potential of Potato Cells

3564 words - 14 pages total volume of 100cm³ of sucrose solution (for the sake of argument). I will also dry each chip before weighing after being in a solution, as the chip will be heavier due to the liquid if it is dripping. I will ensure that the potato chips will have no skin on them at all by peeling the potatoes before the experiment begins, as the skin cells of the potato may have different properties. Preliminary Work Before I carry out my experiment

The Effect of Different Strength of Salt Solutions on Potato Cells Through Osmosis

3013 words - 12 pages The Effect of Different Strength of Salt Solutions on Potato Cells Through Osmosis An Investigation to find out how different strengths of salt solution affect potato cells through osmosis. Aim I aim to investigate the effect of different concentrations of salt solution on potato cells when osmosis occurs. Introduction I intent to set up this experiment using equally sized cylinders of potato submerged

An investigation of the glucose concentration of the cell sap in potato cells

4213 words - 17 pages An investigation of the glucose concentration of the cell sap in potato cells In this experiment I intend to investigate the effects of osmosis on potato cells. Specifically, I intend to use my knowledge of osmosis to investigate the glucose concentration of the cell sap in potato cells. Osmosis is a method by which water levels on either side of a semi permeable membrane may balance themselves. It occurs between regions of high water

The effect of osmosis in potato cells with different concentrations of sucrose solution

2249 words - 9 pages wider perspective on this subject, I would like to repeat the experiment again but using a different vegetable or fruit, for example, with a carrot or an apple to see if osmosis affects them in the same way as it does potato. I would also widen the range of sugar concentrations to include 1.1M 2M. Moreover, I would like to see if and when the cells reach a maximum and minimum weight. Temperature could also be changed, for example the

Similar Essays

The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase

1896 words - 8 pages The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Introduction This is a real A-level school project and as such is intended for educational or research purposes only. Extracts of this project must not be included in any projects

The Effect Of P H On The Activity Of The Enzyme Amylase

1658 words - 7 pages The Effect of pH on the Activity of the Enzyme Amylase Aim :- To find the effect of pH on the activity of the enzyme amylase. An enzyme is a type of protein found in all living cells. Enzymes act as biological catalysts, breaking down substrates without needing a high temperature, allowing all the chemical reactions of metabolism to take place, regulating the speed at which they progress, lowering the activation

To Investigate The Effect Of Surface Area On The Rate Of Reaction Using The Enzyme Catalase, Found In Potato, And The Substrate Hydrogen Peroxide.

2672 words - 11 pages substrates are bonded to make one product causing an anabolic reaction.There are five factors that effect enzyme activity. They are:·Temperature·pH·Inhibitors·Substrate Concentration·Enzyme ConcentrationThe factor I am changing is surface area of potato containing Catalase. By increasing the surface area of the potato, you make more Catalase molecules be exposed to hydrogen peroxide. This will result in the rate of

To Investigate The Effect Of Surface Area On The Rate Of Reaction Using The Enzyme Catalase, Found In Potato, And The Substrate Hydrogen Peroxide.

541 words - 2 pages Hydrogen peroxide has an acidic pH which will break down into water which has a neutral pH. This may effect the enzyme action as many enzymes work faster in a neutral environment, the only control over this is the fact that it will happen in each test and to consider its effect at the end of the experiment. I will need to use the same potato and cut a fresh chip at each stage of the experiment as the catalase may become effected by exposure to