Discovering Factors Affecting Protein Denaturation Essay

1009 words - 4 pages

To examine three different ways of denaturing proteins.HypothesisIt was predicted that the physical characteristics of the egg white solution at room temperature would appear clear and normal like a raw egg white. This is because nothing would be done to the egg white. It was also predicted that when the egg white solution gets heated, the protein would denature if the temperature exceeds 65 °C. The protein would solidify, turn opaque, and turn white in colour. This would happen because the heat at a certain temperature will cause the amino acids in the protein to unravel, thus the protein would lose its structure, and this would reveal a colour change. It was also predicted that when acid is poured into the egg white solution, the proteins would denature and turn into a white colour. This would be because it would result in a further straightening effect on the protein chain. It was also predicted that when silver nitrate is poured into the egg white solution, it would result in some sort of precipitate since silver is always precipitating out. Also, since salts are ionic compounds, they disrupt salt bridges in proteins, and so this can also be a factor.It was hypothesized that egg whites will not regain their original characteristics when removed from the heat or acid because if the protein bonds get disrupted, then the protein is no longer viable and cannot perform its function when it is partially or fully uncoiled.ObservationsTable 1: Chart illustrating the pH and appearance of the egg white solution in test tube A at room temperature (25 °C).Test TubeTemperaturePHAppearanceA25 °C8 - BasicClear solution with foam at the top. Contained tiny white particles floating in the egg white.Table 2: Chart illustrating the pH and appearance of the egg white solution in test tube B when heated in boiling water for ten minutes.Test TubeAppearanceBOpaque solution. Cloudy, foamy at the top. Had a creamy colour.Table 3: Chart illustrating the pH and appearance of the egg white solution in test tube C that contained 1M Hydrochloric acid (2.00 mL).Test TubePHAppearanceC2 - Acidic Opalescent solution. Fewer amount of white particles floating in solution. Foam at the top of solution.Table 4: Chart illustrating the pH and appearance of the egg white solution in test tube D containing Silver nitrate (5 drops). In total, seven drops of Silver nitrate was poured into the solution.Test TubeAppearanceDOpaque solution. Foam at the top of solution. Creamy colour.Figure 1: Illustration showing the egg white solution in all of the three test tubes being conducted on.Discussion1. What was the purpose of tube A?The purpose of tube A was to have it as the control variable in the experiment. The quantity of the solution in test tube A was to remain constant throughout the experiment.2. What common result occurred in all three tubes?The common result that occurred for all three test tubes was that the albumen solution had turned white. This was because test tubes...

Find Another Essay On Discovering Factors affecting Protein Denaturation

Enzymes Essay

1407 words - 6 pages substrate is more tightly bound to the enzyme. Enzymes are, however affected by cellular conditions: pH, temperature and salinity. The one being focused on in this experiment is temperature. All of these conditions will affect the structure of the protein, disrupting the sequence of amino acids causing the enzyme to become denatured. Denaturation “occurs because the bonding interactions responsible for the secondary structure and tertiary structure

exam ppr Essay

6672 words - 27 pages .....................................................................23 Factors affecting enzyme activity .............................................................................25 The effect of temperature .........................................................................................25 The effect of pH changes .........................................................................................26 Chemical denaturation

The environmental factors that effect turnip peroxidase

2009 words - 8 pages are said to be "incapable of adjusting to internal temperature so it must be flexible to allow growth to occur when the temperature outside is optimal". To figure out optimal temperature for turnip peroxidase could lead to better methods of choosing climates for the harvest of these turnips. An important term to know when it comes to temperature and the protein property of the peroxidase is the term denatured. Denaturation occurs when an enzyme

Excess dietary protein and Hypercalcuria. calcium.

2306 words - 9 pages ages. However, there are also dietary factors and living lifestyles that contribute greatly to the disease. For more than half a century there were studies and research, which showed that that diets high in protein increase calcium resorption from bone and urinary calcium excretion, thus, increasing the risks for osteoporosis and other diseases (Munger 149-52). First, this paper will give evidences about how diets that are high in protein contents

Effect of pH and Acid Phosphatase

1371 words - 5 pages rate of reaction by up to 1020 over uncatalysed reactions. (Campbell et al, 2012). Enzyme catalysis is dependant upon factors such as concentration of enzyme and substrate, temperature and pH. These factors determine the rate of reaction, and an increase in temperature or pH above the optimum will lead to the denaturation of the enzyme and a decrease in the rate of reaction. There are many phosphatase enzymes but they are classified as those

Beta-Galactosidase and Western Blot

4002 words - 16 pages thickness. When there are high amounts of protein, there are also notable increase in thickness and intensity of bands. Nevertheless, band thickness and intensity are affected by several factors. In the study conducted, the factor that affects the intensity and thickness of the bands is the amount of E.coli strain used. Varying amounts of EMG 26 and EMG 9 strain are placed on each tube. The first and sixth tubes contain 10 microliters of EMG 26

Interferon Regulatory Factors

755 words - 3 pages protein which targets the STATs proteins for the proteasomal degradation by forming STAT-ubiquitin ligase complex (Nishio et al., 2005; Ulane et al., 2005). The down-stream negative effect is via hindering either the antiviral cytokines or the interferon regulatory factors. As such example, the NS protein of Rift Valley fever virus which is capable of inducing degradation for PKR protein in proteasome-dependent manner (Ikegami et al., 2009

Breast Cancer

2351 words - 9 pages is that "Jet lag affects the pineal gland, which controls melatonin (a hormone essential for maintaining the body's internal clock - circadian rhythms). Lab studies have shown that melatonin inhibits the growth of breast cancer cells by affecting receptors for estrogen. Women with breast cancer usually have a low melatonin level which is thought to help protect against breast cancer. (22) Genetics is one of the number one factors in breast


856 words - 4 pages , there are many different types of amyloidosis and so there can be different risk factors associated with the different types. This could include age, other diseases, and family history. Typically, the majority of people who develop amyloidosis are older then 60 (WebMD, 2012). This is common in conformational diseases because they typically have a later age of onset. This is due to the protein having to accumulate over time and at a certain point

nutrition and sport

1136 words - 5 pages carbohydrates or fats.This process is not healthy for our body as they lose muscle mass and lean tissue and most dangerous is the accumulation of high levels of ammonia, which can be toxic.The protein requirements are not increased despite performing strenuous activity. Eating a high protein diet purely is negative because it creates competition which will affect dehydration endurance and performance. Furthermore the protein overload affecting renal

Amyotrophic Later Sclerosis

2056 words - 8 pages There are many known diseases in the world that we live in today affecting a wide range of individuals of different ages, ethnicities, and genders. With each type of disease comes a diagnosis, prognosis, and potential for a cure from one of life’s many ailments. Over the course of time, technology has began to lead the way in discovering as well as treating many disease in which doctors previously knew little about. Amyotrophic lateral

Similar Essays

Food Proteins: Protein Isolation And Thermal Stability

1968 words - 8 pages thermal treatments induce dissociation, denaturation, and aggregation of soy protein (Kwok and Niranjan 1995). During processing, food products containing soy protein isolates are subjected to various thermal treatments to inactivate anti-nutritional factors, remove allergenic determinants, increase digestibility and to obtain desired functionality, such as solubility or textural properties (Mills and others 2001). Whey is a byproduct of cheese

Protein Folding Theory Essay

1014 words - 4 pages active site. Chaperons and chaperonins help guide proteins when they are folding to establish the most stable construct and prevent proteins from folding incorrectly (Figure 2)(Kmiecik, 2011). We know from Anfinsen’s experiment of protein denaturation/renaturation that a protein’s structure and biological activity are diminished with agents such as pH, temperature, ionic strength and solubility. Once the conditions return to normal, the protein

Isolation, Hydrolysis And Characterization Of Casein From Non Fat Milk And Protein Analysis Using Bradford Method

3769 words - 15 pages factors above, it experiences denaturation. It is a process wherein the protein's structure is disrupted. As a result, the protein may partially or completely lose its biological function or activity. Proteins are coagulated when it underwent denaturation because it become insoluble and are capable of precipitation as it approaches its isoelectric pH (pI). Hence, maximum coagulation is achieved at the isoelectric pH of the protein. According to M. N

Purification Of Milk Process And Chemical Reactions

2473 words - 10 pages phosphatase extracted from rabbit ( Lepus townsendii ) liver. African J Biotechnol 10:3157–3162. doi: 10.4314/ajb.v10i16. Thompson LMM, Robert A (1974) Factors Affecting the Activity and Stability of Alkaline Phosphatase in a Marine Pseudomonad Factors Affecting the Activity and Stability of Alkaline Phosphatase in a Marine Pseudomonad. J Bacteriol 117:813–818.