Effect Of P H On Protein Digestion

507 words - 2 pages

Objective: To investigate how pH affects the rate of protein digestionHypothesis: I predict that since pepsin breaks down the material the quickest at a pH of 2 in the stomach, test tubes #3 will reduce the greatest because it has pepsin to further break down the egg. Whereas the other test tubes will have a smaller reduction. I think test tube #2 will have the second largest reduction rate and then #6 because they all will have a greater rate at breaking down the material, Then, I predict the test tubes that did not have pepsin added will come in last in the order of test tubes #4,#1, and #5.Manipulated variable:*Using different solutions to the test tubes (pepsin, distilled water, HCI, and NaOH)Controlled variable:*the same amount of solution poured into each test tube*ensuring that each test tube is cleanResponding variable:*The pH levels and the size of the egg whiteMaterials:Hydronium pH paper6 large test tubes (22*175mm)10 mL graduated cylinder10% pepsin solutionKnifeTweezers or forcepsWax pen1.0 mol NaOH solution1.0 mol HCI solutionHard-boiled eggMetric rulerPlastic glovesProcedure1)Using a wax pen label the test tubes 1 to 62)Measure and cut 2cm cubes of boiled egg white. Place two cubes in each of the test tubes.3)Using the 10 mL graduated cylinder, measure 5mL of distilled water and pour it into test tube #2. Repeat the procedure, but measure and pour 5 mL of HCI into test tube #3 and #4. Rinse the graduated cylinder. Repeat again, but this time measure and pour...

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