Two compounds from cultivation of the endophytic fungus Penicillium sp of leaves of kunyit putih (Curcuma zedoaria (Berg) Roscoe ) have been isolated. The endophytic fungus was cultivated on 5 L of Potatos Dextose Broth (PDB) medium at room temperature (no shaking) for 3 weeks. The cultures were then extracted with ethyl acetate to afford 3.0 g of residue after removal of the solvent under reduced pressure. The extract was separated and purified by silica gel column chromatography (CC) and afforded two pure compounds as yellow oil (compound 1) and yellow crystal ( compound 2). The structure of these compounds were established by detailed UV, IR and NMR spectroscopic analyses and compound 1 as well as comparison with the reported data. Base on spectra analyses the compound 1 was determined as Di-(2-ethylhexyl)phthalate and compound 2 as 5 (4’-ethoxy-2’-hydroxy-5’-methyl-2’,3’-dihydrofuran-3’-il (hydroxy) methyl-4-isopropyl-3-methyl-2-pyran-2-on). Compund 1 is not new compound, but it is new for endophitic fungus from Curcuma zeodoria and compound 2 is new compound.
Endophytic microorganisms that redise in the tissues of living plants and may produce secondary metabolites of biologically active . Novel antibiotics, antimycotics, immunosuppressants, anticancer compound are only a few examples of what has been found after the isolation, culture and purification and characterization of some choice endophytes in the recent past. isolation of their bioactive secondary metabolites of endophytic fungus from plant could be selected mainly something on ethonobotanical history.
Curcuma zedoaria, a medicinal tuber plant belonging to the family Zingiberaceae has been used in the traditional system of medicine . These plant was used for curing stomach diseases, toothache, blood stagnation, leucoderma, tuberculosis, enlargement of spleen, and for promoting menstruation intraditional medicine in Asia . Antiinflammatory activity , antiulcer activity , antimicrobial effect , hepatoprotective activity , and antiamoebic effect  of this plant rhizome have been reported.
In our search of endophytic fungi, many bioactive compound and new compounds were isolated [11-14]. In this paper we reported the isolation and structural identification one known compound namely Di-(2-ethylhexyl)phthalate (1) and one new compound as 5 (4’-ethoxy-2’-hydroxy-5’-methyl-2’,3’-dihydrofuran-3’-il (hydroxy) methyl-4-isopropyl-3-methyl-2-pyran-2-on) (2) of Penicillium sp from the leaves of Curcuma zedoaria. Penicillium species isolated as endophytites usually be found in plants zingiberaceae  and meliaceae family, although in marine organisms, three meroterpenes preaustinoids , A, B, A1, A2, and B1 have been reported to be isolated from Penicillium sp associated with the Melia azedarach . Penicillium commune from the semi-mangrove plant Hibiscus tiliaceus, have been isolated one new compound...