This website uses cookies to ensure you have the best experience. Learn more

Enzyme Lab Report . Needs More In The Discussion Area.

1628 words - 7 pages

Untitled

Properties of Enzymes: Peroxidase

Biology 1406-8011

ABSTRACT:

In this experiment we did four different activities to compare the results of enzymes. The purpose of the first activity was to observe the effects of enzyme concentration by changing the amount of turnip extract. We began this experiment by using 7 test tubes. One of which was the control, three of them were substrates, and the other three were low, medium, and a high concentration of peroxidase. The substrate and low concentration mixture were then mixed and poured into a cuvette. We then put the cuvette in the spectrophotometer and the absorbance changes were recorded every 20 seconds for two minutes. The same procedure was used for the medium and high concentration peroxidase- substrate mixtures. We observed that the higher the concentration of peroxidase, the more absorbent the mixture was. In the second experiment we were experimenting how temperature can affect the activity of the peroxidase. Our purpose was to find the optimum temperature for peroxidase activity. We used eleven test tubes of different volumes of reagents and placed them in various temperatures of water baths to recognize how the different temperatures affected peroxidase activity. We noticed that as the temperature of the water bath increased the level of reaction decreased; therefore having an inverse relationship. The third experiment we were observing how the level of pH can affect the peroxidase. We used different levels of pH buffers mixed with the reagents and then measured the absorbance using the spectrophotometer. We know that pH affects how efficiently the substrate binds to the enzyme. In our experiment there wasn't much change in the different levels of pH. The final activity's purpose was to notice the effect of an inhibitor on peroxidase. We used hydroxylamine as the inhibitor, which resulted in fully deactivating the enzyme.

INTRODUCTION:

The purpose of this lab report is to better understand the relationship of the enzyme itself its reactions, and how anything can essentially affect the activity of the enzyme. The chemical reactions that we studied were under the control of a catalyst (also known as enzymes). An enzyme is a chemical agent that changes the rate of a reaction without being consumed by the reaction itself. Thus meaning the enzyme speeds up the reaction process tremendously. Almost all enzymes are proteins, and these proteins each have a unique shape called an active site. The active site is a specific area of an enzyme that binds the substrate by multiple weak interactions and forms the pocket in which catalysis occurs. This site determines its catalytic effects. Each active site of an enzyme binds to specific molecules and those molecules alone. After the reactant molecule binds with the active site it undergoes the modification called the substrate. A substrate is simply a reactant on...

Find Another Essay On Enzyme lab report . needs more in the discussion area.

Lab Report on Measuring the Rate of Conversion of Hydrogen Peroxide using Enzyme Catalysis

866 words - 3 pages Lab Report on Measuring the Rate of Conversion of Hydrogen Peroxide using Enzyme Catalysis In essence, the main objective was to use chemical titration to measure and then calculate the rate of conversion of hydrogen peroxide (H2O2) to water and oxygen by using the enzyme catalase. Other purposes of the lab were; to measure the effects of changes of temperature, pH, enzymes concentration, and substrate concentration on rates of an enzyme. The

How the base area of a paper boat affects how much mass it can carry - Science - Lab report

2279 words - 10 pages An investigation of how the base area of a paper boat affects how much mass it can carry without sinking Introduction: Buoyancy is very important in all of our lives. It determines whether an object will float or sink, which is vital to the design of boats, life preservers and many more floating objects. A flaw in the buoyancy of an object can lead to catastrophic events such as sunken boats, oil spills and more. When an object is

Enzyme Function Lab, explores the role of enzymes in chemical reactions

776 words - 3 pages and to determine the kinds of cells that contain more of the enzyme catalase.Prior Knowledge:Enzymes are proteins that assist the chemical reactions of a cell by lowering the amount of activation energy needed to start the reactions, thereby enabling the cell to carry out the reactions at a faster rate; enzymes that lower the activation energy are therefore called catalysts. Moreover, the enzyme itself is not destroyed in the process. Hydrogen

Report On Leisure Facilities In the Pontypridd Area

1783 words - 7 pages Report On Leisure Facilities In the Pontypridd Area This report is complied with data collected by interviews and observations made between the 1st January 2003 and the 28th February 2003. Report on: information regarding leisure opportunities available to young people in Pontypridd. Report to: The Welsh Assembly Government. Report from: Coedylan Comprehensive School Council. Introduction

Enzyme Activity in the Catalase

2352 words - 9 pages The purpose of this experiment was to see if there would be enzyme activity that is in catalase, which is found in potatoes. How my partner and me did the experiment was very simple, we put the filter paper filled with catalase concentration in to five sets of hydrogen peroxide. We found that the more concentration of hydrogen peroxide there was the faster it would take for the filter paper to reach the top, and the lower to no concentration

To investigate the effect of surface area on the rate of reaction using the enzyme Catalase, found in potato, and the substrate Hydrogen Peroxide

2672 words - 11 pages substrates are bonded to make one product causing an anabolic reaction.There are five factors that effect enzyme activity. They are:·Temperature·pH·Inhibitors·Substrate Concentration·Enzyme ConcentrationThe factor I am changing is surface area of potato containing Catalase. By increasing the surface area of the potato, you make more Catalase molecules be exposed to hydrogen peroxide. This will result in the rate of

To investigate the effect of surface area on the rate of reaction using the enzyme Catalase, found in potato, and the substrate Hydrogen Peroxide

541 words - 2 pages Hydrogen peroxide has an acidic pH which will break down into water which has a neutral pH. This may effect the enzyme action as many enzymes work faster in a neutral environment, the only control over this is the fact that it will happen in each test and to consider its effect at the end of the experiment. I will need to use the same potato and cut a fresh chip at each stage of the experiment as the catalase may become effected by exposure to

Action of Amylase in the Small Intestine-experiment-lab report

530 words - 2 pages mark it as no glucose visible on you're data table.Results Table:Conclusion: My hypothesis came out incorrect. It actually only showed signs of glucose after we had poured the solution in the visking tube out. This shows that amylase did brake down the starch but it didn't break it down enough for it to pass through the visking tube and into the beaker. Maybe if we had given it more time, it might have passed a little through it. When we had poured

US Needs More Education in Agriculture

2324 words - 9 pages precious drops that we still have here in the valley are being sold to the Bay Area. If students were being informed of how important agriculture is, they would be more eager to help preserve the current agriculture that is finding a way to thrive here in the Golden State. Agriculture education will not just change a student’s life during their time in school, but will continue to shape their future as they become adults. While in school, students can

Dallas's Ban on Smoking: brief discussion about the recent ban on smoking in Dallas area restaurants and businesses in early 2003. Discussion of health and economic effects

726 words - 3 pages Dallas's Ban on SmokingRecently, Dallas city council voted to ban smoking in area restaurants and businesses. For months, the citizens of Dallas bickered back and forth about the effects of smoking regulations on area economics. These consequences, however, did not stand a chance against the main issue of public health. Health issues of the city's large population are a growing concern for Dallas's mayor and city council. The city government has

The World Needs More Nuclear Power

1082 words - 4 pages attractive alternative to coal and natural gas. Chernobyl and Three Mile Island are two bloody shirts that opponents to nuclear power have waved in their arguments against its proliferation. Chernobyl was a nuclear disaster in Russia that exposed the surrounding area to a severe amount of radioactive material. While the immediate damage and long term effects were severe, there were several factors involved in the accident that made it much worse

Similar Essays

Enzyme Lab Report

3200 words - 13 pages . Then, we continued with the temperature experiment by removing the test tubes from the environments we placed them in and added 1ml of lactase to test tubes A-C using a micropipette. We then, placed Biology 5: Enzyme Lab Report Rivas 7 back to the environments they were previously in for 2 more minutes. Once the time was up we tested for glucose concentration by

Lactase Enzyme Lab Report

2103 words - 8 pages Laboratory 6: Lactase Enzyme Lab Report Jollie Nobleza Biology 5 Dr. Liu March 31, 2014 Introduction Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment

Enzyme Kinetics Lab Report: The Reaction Rate Of Enzyme, ' Amylase In Starch Iodine Solution At Different Temperatures And P H Levels

1996 words - 8 pages Abstract:This experimentation was to evaluate absorbance and the reaction rate of an enzyme, '-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures, and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with

Dell Ads The Biggest Area Of Expansion For Dell Is The Consumer Market. In Order To Develop More Sales In This Market Dell Needs To Focus More Advertising

1937 words - 8 pages dell adsThe biggest area of expansion for Dell is the consumer market. In order to develop more sales in this market Dell needs to focus more advertising on the needs and wants of the consumer. Currently Dell promotes its direct model, which is a key factor Dell needs to create a better position for itself in the consumer market. With all of the PCs in the market being more or less equal, Dell needs to focus on what differentiates its products