This website uses cookies to ensure you have the best experience. Learn more

Enzymes (An Experiment) Essay

1152 words - 5 pages

Problem The phenomenon that we were looking at was how enzymes in potatoes react with substrate. The enzyme will react with the substrate and use the substrate up but the enzyme will regenerate until the substrate is used up. The reaction for this can be written as: E+S ES E+P Where E is the enzyme, S is the substrate, ES is a stage called enzyme-substrate, and P is the product. This reaction in the body is continuous and always equal. The question that this experiment is going to prove is how does the temperature affect the rate that the enzyme and substrate reacts? My hypothesis was when at the temperature was at 21°C, room temperature, the enzyme and the substrate will produce the most product and produce it over a longer amount of time. When the temperature of the reaction takes place at 5°C, the reaction will take a long time to develop but will not produce much product. At 60°C the reaction will start very quickly and produce the majority of its product right away and then will produce very little after the first few minuets.Materials and Methods The materials used to complete this lab were; a blender to make the potatoes into liquid, a spectrophotometer which measured the amount of light was passing thought the product and the numbers could be used to tell how much product was being made, cold distilled water used to mix with the enzyme, since the enzyme came from a potato which is used to being kept at a cool temperature so the water needed to be cooled, room temperature distilled water to make the substrate, buffer was used to also used to make the substrate, and piece of cheese cloth to filter the liquid from the solid after putting the potatoes through the bender. The variables that played a role in this lab were the dependent variable which was the amount of formation of product and the independent variable which was the temperature the reaction was formed. The standardized variables were; all the spectrophotometers had a tube, with no enzymes in the tube, inserted and measured the light passing through and tore it so that the spectrophotometer read 100% of the light was passing through, in every trial the buffer had the same pH of 6, and the last major standard variable was that the same amount and kind of buffer and enzymes were used in each trial. The procedure of this lab was that we first made the buffer. The buffer was made by adding 5 ml of catechol, 5 ml of buffer, and 15 ml of room temperature distilled water. 2 ml of make buffer was added to 2 ml of enzyme in a test tube then shook 10 sec before the first reading. The test tube was then put into the spectrophotometer and the amount of light that is passing through the tube is read. This number tells you how much product has been made up to that point. Every 30 seconds for 10 minuets this test tube needs to be shaken and then put back into the spectrophotometer and have the reading be taken. This whole procedure...

Find Another Essay On Enzymes (An Experiment)

Enzymes Essay

1194 words - 5 pages , reaction vessel and an inverted graduated cylinder. The diagram below (figure-1) further explains the parts of the design. (note all three experiments use the same set up design but had different variables specific to each experiment.)figure-1Concentration experiment- For this and every experiment the this procedure was repeated except for some variables specific to each individual experiment. First a cow liver was chopped up to serve as the

Enzymes Essay

1407 words - 6 pages based on what enzymes were present within the pineapples being utilized. The research also revealed the factors that limit an enzymes functions and how they can affect the function of an enzyme. Through this experiment the whole topic of enzymes was covered. Works Cited 1. Alma, Lori. "Enzyme - Definition of Enzyme." Cystic Fibrosis - Information About Cystic Fibrosis. The New York Times Company, 2012. Web. 05 Jan. 2012.

Coursework and practical plan for title "Investigate the effect of temperature on the action of the enzyme diastase on starch."

1003 words - 4 pages For my investigation, I plan to examine the effect temperature has on enzyme action involving the biological catalyst- diastase, and its substrate- starch. In lesson, I have conducted a preliminary experiment to give me an idea of what to expect and a chance to conduct the experiment before hand. From my preliminary work I believe that an equal amount of substrate (Starch) with an equal amount of enzyme (Diastase) should finish reacting at


1597 words - 6 pages these reactions possible by lowering the reactionÂ’s activation energy. Each cell has tens of thousands of different enzymes that collectively allow both the break down and synthesis of molecules to drive all cellular processes. This investigation will explore the effect of pH on the three-dimensional structure of a protein. Much of the three-dimensional structure of an enzyme is held together by weak


590 words - 3 pages assay mixture. The amount of the second and third enzymes was essential. Likewise, this would have had an effect on the rate of the measured aldolase activity. Hence, the concentrations for both enzymes was measured and calculated prior to the experiment whilst preparing the assay mixture. Furthermore, in order to measure thiol group reactivity the base line was adjusted to zero for each cuvette. This was done to make sure that the results were not affected by the prior readings due to the tiny absorbance of urea.

Biology AT1 'rate Of Reaction' Planing And Obtaining

687 words - 3 pages why enzymes such as pepsin can only reside in the stomach as they can only work in acidic conditions (PH 1-3).concentration: This is the factor i will be concentrating on in this experiment. If there are more amalase particles during the reaction the substrate will have an abundance of molecules to link with and a higher chance of collision thus allowing the reaction to be completed. If the substrate is at a higher concentration than the enzyme

Lab Report on Measuring the Rate of Conversion of Hydrogen Peroxide using Enzyme Catalysis

866 words - 3 pages lab was also an opportunity to see a catalyzed reaction in a controlled experiment. And the last objective was to learn how environmental factors affect the rate of enzyme catalyzed reactions. Enzymes are proteins produced by living cells. They act as catalysts in biochemical reactions. A catalyst speeds up the rate of a chemical reaction and makes it possible for the reaction to occur with a lower initial input of energy. One benefit of

Research Task

1426 words - 6 pages Introduction (Rationale) I have chosen this topic because enzymes are an essential part of human’s everyday life. We, as humans, produce more than 10 000 enzymes and each one has its own unique and specific use in our body. Most of those enzymes are used in our metabolism and, being a fitness maniac, I am very interested to know how my metabolism works and how it differs from others. I will be doing quantitative research on the enzyme

Lab: Enzymes – Protein Catalysts

922 words - 4 pages Lab: Enzymes – Protein Catalysts Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and

The Effect of Temperature on the Rate of an Enzyme Catalysed Reaction

1779 words - 7 pages Aim: To find the effect of temperature on enzymes, using a potato as a catalyst. The source of catalase is in the potato cells.2H2O2 → O2 + H2OPlanning:Introduction: An Enzyme is any one of many specialised organic substances, composed of polymers of amino acids, that act as catalysts to regulate the speed of the many chemical reactions involved in the metabolism of living organismsEnzymes are classified into several broad categories, such

Enzyme Assay: Brain Phosphatases

1869 words - 7 pages phosphatase enzymes from a crude fraction of rat brain tissue. These enzymes are of particular interest in the regulation of numerous intracellular processes, such as protein phosphorylation in signal transduction. There are two broad categories of protein phosphatases: (1) serine/threonine phosphatases and (2) tyrosine phosphatases. The main emphasis of these categories in regards to this experiment will be toward the serine/threonine phosphatases

Similar Essays

It Is A Laboratory Essay On An Experiment We Did Over Enzymes And The Effects Of The Parameters On Enzymes

1436 words - 6 pages had an optimum level where the enzyme activity was at its peak (neutral ph 7, high substrate concentration, 3% salinity rate, and 11 degrees Celsius) INTRO The research conducted was preformed to determine the effects of different parameters of enzyme activity. By doing this experiment we hope to find the best conditions for enzyme activity. Enzyme activity can vary greatly depending on the conditions in which the reactions occur. By changing these

Enzymes Enhances The Digestive System Essay

622 words - 3 pages , proteins transform into amino acids known as pepsin, and lipids are converted into fatty acids and glycerol. All of the enzymes within the digestive system have roles and functions that are very particular. There is an enzyme specifically for lipids, proteins, and carbohydrates, they do not share the same enzymes. The enzyme particularly for carbohydrates is called amylase. Amylase is an enzyme made in the pancreas that helps in the decomposition

The Effect That Temperature Has On Enzyme Activity

1622 words - 6 pages seconds, using the pipette a drop of the solution is added to the iodine in the spotting tray. The colour that the iodine changes to should be recorded. Keep recording the results every 30 seconds, until the iodine solution remains brown. The experiment should be repeated for accuracy and so that an average can be found. Repeat the whole experiment for water baths at temperatures of 20C, 40C, and 80C.This method is an accurate way of carrying out

Investigating The Temperature At Which Soap Powder Will Perform Best At

1642 words - 7 pages Investigating the Temperature at Which Soap Powder Will Perform Best At Introduction I am going to conduct an experiment in the controlled environment of a laboratory. The experiment will consist of placing a stained piece of fabric into water with a fixed concentration of a biological washing powder (Wizzo) in it. I will try this experiment several times trying different temperatured water and leaving the it for a