Identification And Characterization Of Somatic Mutations Associated With Progression Of Acute Myeloid Leukemia Having Flt3 Itd

691 words - 3 pages

My thesis dissertation titled “Identification and characterization of somatic mutations associated with progression of Acute Myeloid Leukemia having FLT3ITD and screening of small-molecule inhibitors in treatment of AMLFLT3ITD” at Cancer Science Institute (CSI) of Singapore, National University of Singapore (NUS) was the ideal experience to acquire skills in molecular cloning, cell culture, protein biochemistry, bioinformatics and exome sequencing.

The molecular pathogenesis of Acute Myeloid Leukemia has not been completely deciphered yet but develops through a multistep acquisition of a wide range of somatic mutations. Therefore, investigation of cancer genome sequences and structures provide insights for understanding cancer biology, diagnosis and therapy. Recent progress in understanding the biology of this disease and identification of driver mutations has ushered in a new era of molecular therapeutics. In this study, we observed several such somatic mutations and the role of these mutations in the progression of the disease. We performed whole exome sequenced on 11 cases of AMLFLT3ITD from diagnosis (Dx) complete remission (Cr) and relapse (Rel). We validated approximately 10-12 mutations per patient. We validated total 137 mutations in 132 genes from 11 cases by Sanger sequencing. These 137 mutations include single nucleotide variation (SNVs), small insertions and deletions (Indels). 60 mutations were present only in Dx and 20 were validated as Rel specific. In addition to this we observed 52 were present in both Dx and Rel. We identified and verified mutations in FAT1 in FLT3ITD subtype and studied the biology of these mutations in AML cells having FAT1 knockdown and overexpression resulting in a tumor suppressor-like activity. We are studying biology of mutations in MLL3 likewise.
Another part of this project was screening for small-molecule inhibitors in the treatment of AML FLT3ITD subtype. I screened set of 100 compounds and studied the effect of each on AML cell lines and traced signaling mechanism in few. Both these projects are in preparation for publication.

During this period, I was involved in two other projects on biology of Lamc2 in tumor progression of Anaplastic thyroid cancer and Pancreatic Cancer. The Thyroid Cancer study titled “Laminin-5 gamma-2 (LAMC2) is highly expressed in anaplastic thyroid carcinoma and is...

Find Another Essay On Identification and Characterization of Somatic Mutations Associated with Progression of Acute Myeloid Leukemia Having FLT3ITD

Critique: Patterns of Hospital Resource Utilization of Children with Leukemia and CNS Tumors

754 words - 4 pages The article “Patterns of Hospital Resource Utilization of Children with Leukemia and CNS Tumors: A Comparison of Children Who Survive and Those Who Die within 3 Years of Diagnosis” is a clearly written article. It identifies the variables as differences between children with leukemia and CNS tumors, in relation to cost, and resource utilization of children who either survived or died after 3 years. The population is stated as children from age

Medical Technology and the Controversies Associated With Prolongation of Life

3168 words - 13 pages have been nearly eradicated (Public Broadcasting System, 2009). The rate of deaths due to cardiovascular disease has decreased 41% in less than 50 years (National Institutes of Health, 2009). End stage renal disease is fatal within months, but with dialysis, patients can live another 5 to 10 years (Merck, 2009). Clearly, medical technology has given countless patients and their families many years together.Medical care is expensive and not

COMPARISON OF HIDDEN HEALTH RISKS ASSOCIATED WITH INCINERATORS AND LANDFILLS

6706 words - 27 pages Health Risks PAGE 23 Running Head: COMPARISON OF HIDDEN HEALTH RISKS ASSOCIATED WITH INCINERATORS AND LANDFILLSCOMPARISON OF HIDDEN HEALTH RISKS ASSOCIATED WITH INCINERATORS AND LANDFILLS[The Writer's Name][The Name of the Institution]Comparison Of Hidden Health Risks Associated With Incinerators And LandfillsChapter # 4Since the dawn of time, we, persons have forever been depending in a number of way or the other on the Earth's capital and

Problems Associated with Cost and Quality of Day Care

1118 words - 4 pages because one does better than another. Both sub-optional and high-quality need to complement each other by having great learning activities and the cost should be cheaper. Families need to be able to pick a great daycare and not feel that it is a burden because of the hassle to find one with good qualities. Another thing I want to change is the prices so that people can budget the cost into the family’s expenses without it being a problem

Symptoms and Progression of Progeria

828 words - 4 pages symptoms of Progeria occur throughout the first year of a child’s life. As he or she gets older, he or she develops into a person with distinct physical characteristics including: baldness, aged-looking, or wrinkled, skin, a pinched nose, and a small face and jaw in comparison to the size of his or her head. Because growth failure is also associated with Progeria, the child will be shorter and weigh less than other children who are the same age. Due to

Identification of Cuminoids with Anticancer Activity

1584 words - 6 pages project has the potential to lead us to novel anticancer agents. With this research I also plan on furthering my education in chemistry and becoming familiar with lab techniques and procedures such as chromatography, identification and structural characterization of natural products, and tissue culture. Results of this project will be submitted as a report and apply for presentation at the 2008 Undergraduate Conference since the data may not be

Types of Horror Associated with Media

1206 words - 5 pages Rough Draft Essay Topic: What are the types of horrors associated with media? As the main topic suggests, what are the types of horrors associated with media? First off, you might want to think about what your definition of fear is, what unsettles you, and what rubs you the wrong way. Well according to Stephen King, this can be broken down into three types; which is the gross-out, horror, and terror. The gross-out in this case is what it is

Leukemia: Cancer of the Blood

1634 words - 7 pages progression of the disease are slow. Fatigue weight loss, and weakness are the symptoms associated with CML. The diagnosis of CML is often made by the discovery of marked granulocytic leukocytosis and extreme spleen enlargement. “Gleevac,” is a new designer drug used to treat CML. Bone marrow transplants cure 70% of all cases of CML. CML accounts for 15 to 20 percent of all leukemias (Bozzone 64). Finally, Acute Myeloid Leukemia is characterized

The Progression of Genetic Engineering and Cloning

746 words - 3 pages Imagine having to explain to your child why they don’t look like you because of you’re selfishness. Imagine thinking your going to design your baby and turns out it has severe leukemia because scientists didn’t put together your baby right. Where Genetic Engineering and Cloning is headed this is possible. Not only is this effecting your child its affecting the whole world as well. Many people think they only do this in humans it happens in

Pozzo and Lucky: Progression of Time

1562 words - 7 pages himself sees schedules as not important but rather time in general not important. Pozzo continues on with life without ever remembering the day before because the past isn't important, and neither is the future, just what he is currently doing. Beckett points out in First Love and Other Shorts “No, I regret nothing, all I regret is having been born, dying is such a long tiresome business I always found” (Beckett 43), which shows again the lack of

Nuclear bombs and the progression of them

751 words - 3 pages Over the last decade there has been a traumatic increase in terrorism. As the amount of terrorist threats and attacks increase, so does the creation of new weapons. Nations are competing with each other to create more destructive weapons. These weapons of mass destruction are essential to modern way of life because they provide protection against terrorist nations, and security to the countries in turmoil.When the atomic bomb was created, people

Similar Essays

Acute Myeloid Leukemia Essay

1794 words - 8 pages When a person is diagnosed with Acute Myeloid Leukemia, they will have many good questions. A question such as; how does the disease work, or what is the science behind the condition? How dangerous is the disease? What different types of AML are there and how are they different? What treatment option will work the best? But perhaps one the most helpful questions of them all may be: “How challenging is this disease to cure?” One of the easiest

The Development Of Novel Drugs For Chronic Myeloid Leukemia

1002 words - 5 pages a disease [10]. However, there are still a certain group that says biomarkers can also be imaging scans [10]. They play a role in the discovery of causes, diagnosis, prediction and treatments [4], and also monitor the body’s responses to drugs during a treatment. Bcr-Abl oncoprotein is a well-known molecular marker with a constitutively activated tyrosine kinase domain. In addition, it has two loops stabilized that are targets of mutations, the

Mutations Of The Superoxide Dismutase Gene Are Associated With Amyotrophic Lateral Sclerosis

1771 words - 7 pages ]. Of the exons studied, no changes were found in exon 3, but mutations were found in exons 1,2,4, and 5 (single base pair changes) [4,16]. For their identification PCR and SSCP were analyzed. Restriction enzymes have been used to find restrictions sites that can be altered by various mutations of SOD. The fragments that were found in the mutated genes were compared to fragments formed from the same procedure with a normal patient control

Evaluation Of Chromosomal Aberrations In De Novo B Lineage Acute Lymphoblastic Leukemia: Determination Of Frequency, Distribution Pattern And Iden

1082 words - 5 pages patients with a five year EFS of >90% in developed countries (4) and of 45%-55% in India (5) and the future is encouraging. Identification of alterations both at the chromosomal and molecular levels is presently a key thrust area in acute leukemia. ALL diagnosis is based on cytomorphology, immunophenotype, karyotype and molecular genetic analysis of the blast cells in the peripheral blood and/or bone marrow (6,7). Over the last few years world-wide