Experiment To Establish The Link Between Light Dependent And Independent Reactions

520 words - 2 pages

2

[Type text]

Practical Laboratory Experiment John SimmonsAn experiment to demonstrate the link between light dependent and independent reactions in plant leaf matter.IntroductionThis practical experiment is to determine whether leaf material may be used to show photosynthesis.In light dependent reactions, NADP is the reducing agent (it loses, or donates an electron and is oxidized) which is then used to produce reducing power for light independent reaction.Isolating chloroplasts, the cell organelle which has the primary function is photosynthesis, will test this hypothesis, and subjecting it to lit and dark environments in order to record and analyse the results.Solutions of other materials used in the experiment will also be tested as a control.MethodThe chloroplasts is isolated by grinding the leaf material (spinach),using a pestle and mortar, and the resulting pulp or brei, is sieved and equally divided into centrifuge tubes and separated by centrifugal force for ten minutes.Next the supernatant, the residual liquid above the chloroplast pellet, is poured into a separate container and the chloroplast is suspended with isolation medium and remixed. This is then kept cold until use.Five labelled tubes are then prepared using the suspended leaf material, numbered one to five.DCPIP (2,6, Dichlorophenolindophenol), is a blue dye, acts as an electron acceptor and becomes colourless when reduced. Therefore, when this is added to isolated chloroplasts, any reducing agent produced by them may be detected.The DCPIP should be used at room temperature.Two of the tubes containing leaf material are placed under light and one in darkness. Readings are then recorded using a colorimeter, noting time and environment. Other tubes containing no chloroplast pellets are used as a control.The table below (a) records the contents and environment of the tubes used in the experiment.
Tube

Leaf extract/cm3

supernatant

Isolation medium

Distilled water

DCPIP...

Find Another Essay On experiment to establish the link between light dependent and independent reactions

The Link Between Cancer and Mitosis

838 words - 3 pages The Link Between Cancer and Mitosis Cancer is one of the most common diseases in the developed countries, accounting for around one in four deaths. In Britain it caused about 25% of the deaths h in 1991 and is the most common cause of death following cardiovascular disease. There are, in fact, over 200 different forms of cancer, which is why it is not necessarily thought of as a single disease. For example, breast

The Link between Crime and Mental Disorder

2255 words - 9 pages accurate. Much research on the link between crime [specifically to this discussion, violent crime] and mental disorder has been conducted, however there are different factors that need to be taken into account when examining this link. Definition of mental disorder is a major one, along with possible uneven sample distributions. Also, there have been changes in mental health and criminal justice policies that have increasingly made

The link between creativity and mental illnesses

2201 words - 9 pages . Artists such as poets, writers, painters, designers, and more use creativity on a regular basis. They use creativity to come up with new ideas and create original works of art. A poet would use his or her creative abilities to write and produce a poem just like a painter would use creativity to paint a painting. There is a magnitude of research put behind trying to find the link between creativity and mental illness. “Creative individuals

The Link Between Globalization and Poverty

2936 words - 12 pages The Link Between Globalization and Poverty The word globalization gets tossed in conversation with out even slipping our minds what it is. But what is it? Globalization is the concept of companies sending jobs overseas to those who will work for less. Globalization is the ability to go to McDonald’s in China and eat a Big Mac that tastes as if you ate it on your homeland. Globalization allows you go on Facebook to communicate with your exchange

The Link Between Teaching, Learning, and Behaviour

1742 words - 7 pages In my opinion, pupil’s behaviour relates to the way they see their school. For example, if the school is modern and refreshing, generally pupils will respect the school and the people in it. In contrast, a school that is run down and old, pupils may think this is a reason to abuse the school and the not care much for it. The school on my first placement (BP1) was built in the 60’s but has had recent modifications and new buildings built

The link between Relegion and God.

1119 words - 4 pages The existence of God has been a question since the idea of God was conceived Descartes tries to prove God's existence, and to show that there is without a doubt something external to ones own existence. He is looking for a definite certainty, a foundation for which he can base all of his beliefs and know that they are true. Descartes' overall project is to find a definite certainty on which he can base all his knowledge and beliefs.Descartes

Similarities and Differences Between Who Wants To Be A Millionaire? And The Weakest Link

1894 words - 8 pages Similarities and Differences Between Who Wants To Be A Millionaire? And The Weakest Link There are both similarities and differences between the two quiz shoes, they both have the basic format of a quiz show, the entertain, excite and amuse us which is a very basic formula for any good television program, however, they are dissimilar in many other ways. The similarities between 'Millionaire' and 'Weakest Link' could be

To what extent does research support a link between stress and the immune system?

867 words - 3 pages There has been extensive research into the link between stress and the immune system that shows that the link is indeed very strong with a positive correlation. The research is seen in the general adaptation syndrome, Stone's experiment on stressful events and the links to symptoms, Cohen et al experiment on the common cold, and Keicolt Glaser who looked into immune function.Stress has been found to suppress the immune system allowing people to

The Contrast Between the Daily Mail and the Independent

1168 words - 5 pages The Contrast Between the Daily Mail and the Independent Newspapers need to aim at a specific corner of the market to be successful. The main reason for this is that if you have a readership consisting of a specific class or type of people eg. Middle class business men. Then advertisers who want to appeal to that specific audience will use that paper for their advert and the paper can obtain large profits from adverts

The Link between the Teacher-Student Relationship and Academic Achievement

2510 words - 11 pages student does not see that. Instead, the student views their academic motivation and achievement as solely their own doing. Teacher-student relationships: A growing field of study “Many teachers in comprehensive high schools believe it is important to create social distance between themselves and their students as a means of maintaining discipline and helping students to become more independent, responsible and mature” (Wehlage, Rutter, Smith

The Link Between Nazi Propaganda And The Holocaust

2880 words - 12 pages Hitler believed that propaganda from the allies was the main reason that the Germans lost during World War I and felt that this form of warfare needed to be a primary tool in modern warfare. He spoke of this belief in his book Mein Kampf well before the start of the second World War. Hitler felt that the public needed to be inundated with the ideology of the state at all times and through all mediums (Jowett and O'Donnell 2). "To do this," he

Similar Essays

The Link Between Autism And Immunization

946 words - 4 pages A recent spike in the number of diagnoses of autism spectrum disorders has also brought to light the controversy that exists concerning the link between autism and immunizations. In a piece published by CNN, Jenny McCarthy depicts her son’s recovery from autism. In it, she claims that autism is an entirely environment illness, and states that vaccines are a major trigger of the disease. A Newsweek article printed in 2005 discusses the search for

The Link Between Painting And Life

1091 words - 4 pages practice of the formal element value, the degree from light to dark. It is obvious where the light and dark portions of the painting are, but what makes the value in this painting really pop is that there is no gradient between light and dark. What is dark is almost pitch black and what is light is very illuminated. The lack of a gradient leaves such a large gap between shading and highlighting that the other formal element space can be seen in this

The Link Between Creativity And Bipolar Disorder.

670 words - 3 pages QUESTIONThe relationship between creativity and mental illness is widely recognized in the medical field, although little research exists under the conditions of a controlled experiment. Studies exploring the facets of the prescribed cognitive function are centered on afflictive illness, specifically bipolar disorder. Consequently, the objective of the present research is to demonstrate the varying levels of creativity displayed by bipolar

The Link Between Quality And Learning

1494 words - 6 pages The use of the number of units produced, as the only independent variable to monitor the progress of a task, has raised important questions about the effectiveness of learning models. The traditional learning curve, initially developed for airplane production, proved to be impractical for many other engineering processes since it implies that all units produced are of acceptable quality. Seeking a link between learning curve tools and quality