Experiment To Test Two Different Concentrations Of Dettol Disinfectant In Soil (Sand) Bacteria Growth

1052 words - 4 pages

The aim of this experiment is to test two different concentrations of Dettol disinfectant in limiting soil (sand) bacteria growth.

I think that the concentrations with the most Dettol disinfectant will stop bacterial growth the most. Out of all the percentages I think the 100% disinfectant will stop the bacteria the most, this solution would be made from 10ml of Dettol disinfectant and 0ml of water.

The independent variable in this experiment are the amounts of Dettol disinfectant used for each petri dish, the dependant variables are the measurement of bacterial growth that grows during the time and the controlled variables are everything we kept the same such as the amounts of agar solution, the beaker size, same sized petri dishes, same soil and same sized filter papers.
• Dettol disinfectant
• Measuring cylinders
• Beakers
• Labels
• Soil
• Agar
• Petri Dishes
• Filter paper
• Sticky tape

1- Collect the Dettol disinfectant and water quantities based on the concentration you were given to make. Use 10Ml measuring cylinders.
2- Prepare your disinfectant concentrations into 100mL beakers.
3- Prepare soil and water together allowing soil to settle at the bottom, the pour 10mL of this water without getting any of the soil into the beaker, get rid of the remaining soil and water.
4- Collect a beaker of 50mL full of agar from your teacher and then mix the 10mL of soil water with the agar carefully.
5- Pour the agar/soil water into the petri dishes evenly.
6- Allow the agar to cool and set.
7- Soak your small disk of filter paper into the disinfectant concentration, let it soak for about 10 minutes.
8- Once soaked, place the filter paper into the middle of the agar plate carefully, close the lid and seal with sticky tape, label your petri dish and return it to your teacher.
9- Leave petri dish for a few days in the incubator.
10- Collect your results.

With the results of the agar plates we can see that the solution with the most disinfectant (100%) had hardly any bacterial growth at all, where the (0%) solution with no disinfectant had lots and lots of bacterial growth. Most of the other solutions had growths also, my given solution of 75% had small amounts of bacteria around the outside of the agar plates. The main growths of bacteria occurred around the edge of the filter papers.

Each bacterial growth grew in small shapes and sizes with no pattern in the middles; there was a very strong smell that could be smelt from about 20cm away from the petri dish.

Surprisingly enough there was a significant difference in bacterial growth with the 50% and the 75% since there Is only 25% disinfectant difference. The 50% solution had a lot more bacterial growth compared to the 75% that had only small amounts.

Discussion –
I observed that the agar plates grew bacteria over a number of days for all of the agar plate experiments even with the 100% bacterial Dettol and no other Parts to the...

Find Another Essay On Experiment to Test Two Different Concentrations of Dettol Disinfectant in Soil (sand) Bacteria Growth

Investigation to find the effect of different concentrations of sugar solutions on osmosis

1930 words - 8 pages , measuring cylinderPlan: A range of sugar solutions will be prepared with concentrations from 0.2 M to 1.0, in intervals of 0.2, by adding varying amounts of distilled water. Sections of potato will be cut using a cork borer and knife to equal lengths, this will keep the surface area constant and their mass measured. 10 ml of each concentration of sugar will be placed in three test tubes and labeled. To each test tube a cut piece of potato will be added

The effect of osmosis in potato cells with different concentrations of sucrose solution

2249 words - 9 pages controlled using film secured on the top of each of the test tubes to prevent uneven loss of water from disturbing the experiment. The independent variable is the alteration of the concentration of the sucrose and distilled water in the solution that the potato will be suspended in. I will select different concentrations that will provide the most efficient results for me to observe the effects. Volume of sucrose

Investigating the Size of Craters in Sand when a Marble is Dropped into the Sand from Different Heights

1958 words - 8 pages Investigating the Size of Craters in Sand when a Marble is Dropped into the Sand from Different Heights * Metal container * Sand * Marble * 1 metre rulers * 30cm ruler Method * First of all I collected all the equipment I needed. * Next I put the tub of sand next to the wall and leaned the ruler up against the wall resting on the sand. * I then took the marble and

Two different countries in two different continents. One of them

1340 words - 5 pages . Although they are located in different region and different continent in the world these two countries both have the same problems. These two countries are members of International Money Fund. However there are lots of similarities between Turkey and Congo, these countries are not exactly the same countries. Both of them are taking financial aid from this international institution to develop and overcome some economic problems in their countries such

Identifying Two Unknown Species of Bacteria

1353 words - 5 pages which drops the pH. If this happens a yellow halo will appear around the bacteria growth. The coagulase test checks for a bacteria’s ability to clot serum. If the serum clots then the bacteria possesses coagulase activity. The tests were performed and placed in incubators. The results were checked 48 hours later. Week 2, Day 2 All tests were viewed and the results were recorded. Analysis of the results was made and the bacteria

Types of soil in Malaysia

1583 words - 6 pages results in low cation exchange capacity but a good bearing capacity. Cation exchange capacity is the ability for the soil to exchange ion such as K+ and Fe2+ ion. A good bearing capacity means that Ultisols can bear loads that apply to the ground. This is good for engineering uses such as anchoring the foundation of buildings. There are two factors that make up this soil's characteristics which are climate and temperature. Ultisols are the humid-area

Two Different Reactions to the Adult World: Youths in the Works of Graham Greene

2097 words - 8 pages shows great resolve and never wavers from his intent to destroy Old Misery's house and dispel the air of privilege that surrounds him as he enters into the Gang. In contrast, Master Philip, when confronted with adulthood, is emotionally scarred, in that he witnesses the affair of his butler and the subsequent death of the Butler's wife. These two reactions are very different as Trevor reacts by stepping up to the challenge presented but Philip is

Chemistry Experiment on the Reaction of Different Metals When Exposed to Acid

891 words - 4 pages more reactive. I gathered together four different metals and acid compounds which all reacted with one of the elements in the past, for example, Iron Sulphate (FeSO4) contain iron. I prepared equipment I would use for the experiment, such as a pipette to carry the acid in, tweezers to lift the metals in, trays to pour the acids in, etc. I took an appropriate amount of acid and added in and the tray using the pipette. I then used the

Investigate the rate of reaction between Calcium Carbonate and Hydrochloric Acid at different concentrations

989 words - 4 pages Investigate the rate of reaction between Calcium Carbonate and Hydrochloric Acid at different concentrations Aim: To Investigate the rate of reaction between Calcium Carbonate and Hydrochloric Acid at different concentrations. Background Knowledge: I know from my knowledge of science, that by adding together a carbonate and an acid, that a salt, water and Carbon Dioxide are produced. Therefore in order to measure the rate of reaction, one of

This project will seek to investigate two ponds of apparently different ecosystems in Auchlyne Estate and Whim Village

2192 words - 9 pages Project TitleThis project will seek to investigate two ponds of apparently different ecosystems in Auchlyne Estate and Whim Village. By analyzing the number of organisms present and different parameters, it is hoped to garner conclusive data which will aid in the determination if in fact the specific ecology of each respective pond has an impact on the life present there. Purposes of ProjectTo determine, compare and contrast the environmental

How does Okara in "Piano and Drums" respond to the two types of different music and instruments?

989 words - 4 pages In the poem "Piano and Drums" the narrator: Gabriel Okara, responds to the two types of music and instrument; Piano and drums differently. His response to the drums is that of excitement and youthfulness, however, his response to the piano is of unfamiliarity. Okara shows his two different attitudes through his choice of diction, rhythm, metaphors and symbolism.The choice of diction used to describe the drums and piano creates a strong emphasis

Similar Essays

Lab Experiment: The Effectiveness Of Different Antibiotics On Bacteria

2788 words - 11 pages . Contaminated bacterial culture may affect the growth of bacteria in the culture. Furthermore, the aseptic techniques can prevent infection of bacteria on the experimenter. Controlling of variables: Different antibiotics are used to test their effectiveness on bacteria. The growth of different bacteria reacts differently to different antibiotics. The growth of different bacteria can be quantified by measuring the diameter of the clear area

How Different Concentrations Of Acid Affects The Growth Of The Crest Seed

791 words - 3 pages affect the growth of the plants. This makes me think that the higher concentration of acid will not allow the crest seeds to germinate. My own theory is the lower the concentration of acid, the better the seeds will germinate.Plan: During the investigation I worked in a group of 5. First we collected the equipment for the investigation. Then we collected the different concentrations of acid. We got the cotton wool and rolled into a ball. Then we

Growth Dynamics Of E. Coli In Varying Concentrations Of Nutrient

2609 words - 10 pages time. This equation allows one to calculate the numbers of cells in a culture at any given time. The reciprocal of k is the mean doubling time, in other words, the time required for the population to double, usually expressed as cells per unit volume. (Edick 61-62)Temperature is the most influential factor of growth in bacteria. The optimal temperature of E. coli is 37C, which was maintained throughout the experiment. Aside from temperature, the pH

Gram Staining To Distinguish Between Different Types Of Bacteria

568 words - 2 pages It/'s a practical Good and to the point.Gram Staining to Distinguish Between Different Types of BacteriaIntroductionBacteria fall into two natural groups according to their cell wall structure. Some are stained with Gram's stain and are termed Gram positive, others do not retain the stain during the decolourising procedure and are termed Gram negative.AimTo test whether B. subtilis and A. tumefaciens are Gram positive or Gram negative using the