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Extraction Of Dna From Plant Tissues

1119 words - 4 pages

Manufacturing of Carbonated WaterLee Yew ChernB1400461Running head: MANUFACTURING OF CARBONATED WATER 1MANUFACTURING OF CARBONATED WA TER 9HELP UniversityExtraction of DNA from Plant TissuesObjectivesTo demonstrate extraction of DNA and to study the characteristics of plant DNA.IntroductionDeoxyribonucleic Acid (DNA) is a genetic material that carries biological data for the formation and function of living things. Within the plant species, the DNA molecule houses structures of nucleus, mitochondria and chloroplasts; which the chloroplasts makes plant DNA unique from animal DNA. In a simple DNA molecule, four nucleotides can be found. For each nucleotide it houses five-carbon sugar (deoxyribose), phosphate and one of four nitrogenous bases; which either one could be adenine (A) and guanine (G) the double-ringed purines or thymine (T) and cytosine (C) the single-ringed pyrimidines, as explained by (International Baccalaureate Biology, 2013).The hypothesis in the experiment is that the shampoo will be able to break through the phospholipid bilayer of the strawberry plant cell and DNA will be precipitated when ethanol is added.Materials
1. 100ml Beaker

5. Strawberry

9. Blender

2. Hair shampoo

6. Scalpel

10. Filter

3. Glass rod

7. Ice water bath

11. Ethanol

4. Stopwatch

8. Hot water bath

12. Trypsin

Method3g of salt, 90ml of distilled water and 10ml of hair shampoo were added together in the 100ml beaker while stirring.Chopped strawberries were adding into mixture after it is stirred thoroughly.Beaker of mixture was given an exact 15 minutes of 60ºC water bath.After 15 minutes of hot water bath, beaker of mixture was given an ice water bath.Stirring of the mixture was until temperature of the beaker and mixture cooled down.Mixture was filled into a blender to blender everything thoroughly (Figure 1.1).The blended mixture was poured into a beaker passing through a filter (Figure 1.2).2 drops of trypsin was added to filtrate for every 10 ml.Total drops of trypsin were 12 drops and total amount of filtrate was 60ml.Filtrate and trypsin were mixed well with a glass rod.Ice cold ethanol was slowly poured through the slide of the beaker into the mixture of filtrate and trypsin, forming a layer of ethanol.ResultsBelow here are the observations taken during the experimenting of plant DNA extraction.VARYING DEFINITIONS OF ONLINE COMMUNICATIONThe mixture before strawberries were added (Figure 1.3).The color of the mixture was bright yellow. A lot of bubbles were created due to the presence of shampoo.The mixture after strawberries were added (Figure 1.4).The color of the mixture became reddish pink and cloudy. Bubbles are still present.The blending of the mixture in a blender and filtering of it (Figure 1.5)Significant amount of bubbles amassed due to the blender's quick blending speed. When filtering the blended mixture, lot of bubbles were left on the filter and the finer mixtures became the filtrate which contains DNA...

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