Genome Of Cannabis Sativa Essay

3418 words - 14 pages

DNA sequencing and functional analysis of potential LEAFY (LFY) homolog in Cannabis sativaDanny Perretti & Lisa KlingerSci. Inv. Tech.Dr. ConnorsAbstract Flower development in angiosperms is controlled by meristem identity genes. One such gene is the FLORICAULA/LEAFY gene which is responsible for reproductive transition and flower spatial patterning. It is not known if Cannabis sativa, an angiosperm species, possesses a FLO/LFY homolog that could play a central role in the flowering development of this plant. Through DNA sequencing and analysis, we will determine if such a homolog exists. If so, this FLO/LFY homolog can be isolated and results will provide insight into the largely unknown Cannabis sativa genome and how the flowering of buds is controlled and expressed. This homolog may play a crucial role in the flower development of Cannabis sativa and could serve as a functional flower meristem identity gene in this plant species as well. Through identification and analysis of the FLO/LFY homolog, and presuming it does share the same biological characteristics and roles as the FLORICAULA/LEAFY gene, further functional analysis can be carried out. Spatial expression patterns during flower induction periods will show when this discovered FLO/LFY homolog is being mainly expressed. This information will allow for the possibility of developing a way in which to promote early flower development in the Cannabis sativa species which could be beneficial by increasing mass production and reducing costs brought on by pharmaceutical companies generating Cannabis sativa for medical use.The general subject of interest is identifying and analyzing homologs of the LEAFY gene. The LEAFY gene is a flower integrator gene and an important part of the flowering process in angiosperms. LFY has a part in a plants transformation from the vegetative stage to the reproductive stage, referred to as the floral transition. The LFY gene is also very important in the regulation of flower development (Xu, Han, Pan, & Pang, 2012) . Flower development is dependent on a gene regulated pathway, consisting of three major steps; the transformation of the vegetative shoot apical meristem into an inflorescence meristem, the formation of floral meristems on the inflorescence meristem through the aggregation of undifferentiated cells, and the development of floral organs after floral meristem differentiation. The LFY gene has a specific role in the second step of the flower development process. Considered a floral meristem identity gene, the LFY gene is partially responsible for the production of floral meristems located on the sides of the inflorescence meristems (Liu, Thong, & Yu, 2009). The LEAFY gene's significant role in a plants floral transition is what makes this subject area interesting. Research being done in this field of study can potentially have a huge impact on society. The ability to control the expression of a gene such as the LFY gene makes it possible to...

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