This website uses cookies to ensure you have the best experience. Learn more

Gram Staining To Distinguish Between Different Types Of Bacteria

568 words - 2 pages

It/'s a practical Good and to the point.Gram Staining to Distinguish Between Different Types of BacteriaIntroductionBacteria fall into two natural groups according to their cell wall structure. Some are stained with Gram's stain and are termed Gram positive, others do not retain the stain during the decolourising procedure and are termed Gram negative.AimTo test whether B. subtilis and A. tumefaciens are Gram positive or Gram negative using the Gram staining technique.Apparatus( Stain: : Crystal Violet solution (0.5% aqueous)( Mordant : Iodine solution( Decolouriser : Ethanol 95%( Counterstain : Safranin solution (1% aqueous)( Culture of Bacillus Subtilis( Culture of Argobacterium tumefaciens( Water bottle( Wire loop( Bunsen Burner( Glass slides( Paper Tissues( MicroscopeMethod(1) Take a microscope slide and using a dropper place two or three drops of ethanol on the slide. Using a clean tissue wipe the slide. Replace the dropper in its dropping bottle.(2) Having put on some safety glasses, light the bunsen burner. Take the wire loop and flame it. After flaming the wire loop, dip it in some of the B. Subtilis culture and spread it on the slide to form a smear.(3) Holding the slide at one end, invert the slide and the pass the slide through a flame very quickly until the sample is dry. This technique is know as 'heat fixing'. The slide should then be allowed to cool to room temperature.(4) Next cover the smear with some crystal violet solution. Allow the smear to stain for 1 minute and then rinse with water (from water bottle).(5) Using the iodine solution, place some drops of it on the smear. Allow this to fix for one minute and then rinse with some water.(6) Add some ethanol dropwise allowing the ethanol to run-off the slide. When the run...

Find Another Essay On Gram Staining to Distinguish Between Different Types of Bacteria

Applying Staining Techniques to View and Identify Bacteria

664 words - 3 pages Abstract The main objective of this lab was to identify different bacteria by simple, negative, and gram staining. To view each bacteria cell, the bacteria was transferred aseptically to a slide, and they were then viewed by using oil immersion, by a light microscope. From this lab, it was determined that E. coli and B. megaterium are gram negative and B. subtilis and S. Marcesans are gram positive. Introduction The purpose of this

Peptidomics approach to study bacteriocins from Gram-positive bacteria

554 words - 3 pages ribosomal translation. The structural genes encoding for bacteriocins are associated in clusters with other genes necessary to their production, regulation and immunity mechanisms which make producer strains immune to their own bacteriocins [2]. Bacteriocins, particularly those produced by lactic acid bacteria (LAB), have attracted interest for the applications as safe food preservatives, as they can inhibit growth of many common bacterial species

Distinguish between the law of diminishing returns and returns to scale

876 words - 4 pages scale achieved by the firm will be overridden. However, this managerial breakdown may be because as the firm has grown and the factors of production have increased the number of managers has not increased by the same proportion. In practice, this makes decreasing returns to scale hard to justify, so it remains a theoretical concept.The main differences between the law of diminishing returns and returns to scale are that one is a concept in the short

Different Types of Explosives

1312 words - 5 pages Different Types of Explosives An explosive is something capable of exploding or tending to explode. The energy released is used in firearms, blasting, and rocket propulsion. Explosives are substances that produces a rapid, violent reaction when exposed to heat, a strong blow or a special detonator. During the reaction, explosives give off large amounts of gases at high pressure. The powerful blast of energy

Different Types of Love

1227 words - 5 pages “Love does not begin and end the way we seem to think it does. Love is a battle, love is a war; love is a growing up.” –James A. Baldwin. The author, Baldwin talks about the fact that we assume love starts once you meet that person and it ends when you die, but love is everlasting and is a part of you from the very beginning. In his quote he also says that we will face different types of love, while growing up. Films are made about it, books

Different Types of Accents

1369 words - 5 pages different accents to utilize. The list helps the listener compare the accents to one another because everyone is reading the same list of words. I compared the accents between North Jersey and South Jersey. I came to the conclusion that the differences between the two accents were very subtle. However, when it came to answering the questions, the answers didn’t necessarily touch on the person’s accent, but rather their choice of words. This was a

Different Types Of Procrastination

766 words - 3 pages According to the text, procrastination is avoiding the less important things in one’s field of study or interest and paying attention to the more important things pertaining to one’s field of study. For example, if a student is seeking a degree in psychology and has an assignment due, he or she might forgo shopping to complete that assignment. Good and Bad Procrastination written by Paul Graham, with the purpose being about different types of

Different Types of Power,

1153 words - 5 pages Power is a relationship between people; to clarify A won’t do what B wants unless B has power. Power is to achieve goals against other people will. Power is the ability or potential to influence outcomes in order to achieve an objective. Different types of power are usually blended together in order to achieve the required outcome. First type of power is Force; Force is the exercise of power by physical means. Force can include Physical

Different Types of Suicides

703 words - 3 pages had a death rate of eighteen point six, people ages eighty five and over had a death rate of seventeen point six. There are different reasons why people decide to end their own life. One reason could be because of bullying. Suicide related to bullying formed the CDC (centers for disease control and prevention). In between twenty and fifty six percent of adolescents are either bullied themselves or are the ones who bullies other people

Different types of boyfriends

511 words - 3 pages In life, we all going through many relationships until we find the one who we feel is te perfect mate for us. While we are datin others, we may experience different types of boyfriends. Based on certain characteristics, an excessiv type of boyfriend can be passiv, energetic or prejudiced.First, there is the passive type of boyfriend. He is a person who doesn't like to go out. He spends his free time wathcing TV and sitting on the couch. He is

Different Types Of Disabilities

642 words - 3 pages than normal people and need the empathy and care of family and friends. There are a number of different types of disabilities that can affect any part of the human body. Mental disabilities, mainly affect the brain and interfere with its proper functioning. Physical disabilities affect any part of the body and could even cause paralysis. A few of the common disabilities are listed below. Muscular Dystrophy This one affects the muscles of the

Similar Essays

Bacteria And Gram Staining Essay

1165 words - 5 pages observed to be Gram-negative. It was then determined that this bacteria was staphylococcus.Table 1: Colony type the bacteria was taken from, its reaction to Gram staining, and what kind of bacteria it was observed to be. Colony Type Bacteria Type Gram Stain Cream irregular diplobacillus Gram-positive Red round staphylococcus Gram-negative Yellow round diplococcus Gram-negative DiscussionThe reason a certain bacteria would show

Importance For Tourism Industry To Distinguish Between Different Types Of Tourists

1646 words - 7 pages Importance for Tourism Industry to Distinguish between different types of Tourists1. Discuss why it is important for the tourism industry to distinguish between different types of tourists?The reasons why people are motivated to travel or to go on a holiday and the particular type of travel experience that are seeking when they do so, are wide and varied. They are dependent on a number of disparate and varied factors based on particular

Different Types Of Development Structures And Nature Between Rugby League And Ice Hockey

1413 words - 6 pages years there has been increased attention to establish and improve pathways and systems to maximize sport development. This essay will discuss and critically analyse the different types of development structures and nature between Rugby League and ice hockey as recognised sports of Australia. Australia has, with its old and inherent football-culture of Rugby League/Union and Aussie Rules, been predominately focused on the domestic development and

Isolation Of Gram Negative Bacteria Essay

3957 words - 16 pages . Therefore, it is important to identify microorganisms within the water to avoid disease. Common types of gram negative bacteria that inhabit water are from the families of Enterobacteriaceae, Vibrionaceae, Pseudomonadaceae, Neisseriaceae and Pasteurellaceae. This laboratory experiment will be used to identify a gram negative microbe but do not forget that there are several types of gram positive bacteria that live in the water too!1) ISOLATION OF GRAM