This website uses cookies to ensure you have the best experience. Learn more

How P H Affects An Enzyme's Ability To Catalyze Honors Biology I Lab Report

943 words - 4 pages

2
How pH Affects an Enzyme’s Ability to Catalyze
How pH Affects an Enzyme’s Ability to Catalyze
Kayah Takei and Annelise Kofod
Durham Museum of Life and Science
Honors Biology I
Abstract
The purpose of our lab was to demonstrate how pH affects the productivity of enzymes. In our lab, we tested how changing the pH of our enzyme, lactase, affected its ability to catalyze its substrate, lactose. We tested the enzyme’s ability to function by using glucose test strips to determine if glucose was present. Our results showed no sign of glucose, thus, supporting our hypothesis. These results are important because it shows the environmental sensitivity of the enzyme lactase.
Introduction
Every enzyme has an active site, the area where a substrate will bind to the enzyme to conduct the chemical reaction. When conditions are ideal, the enzyme should catalyze, or speed up, the reaction. Enzymes are very specific as to what reactions they will catalyze. The active site of every type of enzyme is different, allowing the enzyme to catalyze only one type of substrate. This, in turn, makes the enzyme very specific.
In earlier research, after using several potential substrates and lactase as our enzyme, we measured presence of glucose by using glucose test strips. We found no presence of glucose in anything except for lactose, demonstrating the specificity of enzymes.
In this report, we extend our earlier work by changing the pH to test how changing the environment surrounding the enzyme affected it. Our hypothesis was that changing the pH of the substance would make the lactase unable to catalyze the lactose. The goal of this experiment was to determine if lactase would denature in response to a basic pH. A denatured enzyme would be unable to catalyze its substrate.
Methods
We conducted our experiment using sodium hydroxide (NaOH), lactase, and milk. The purpose of the NaOH was to change the pH of the solution. NaOH has a pH of 13, extremely alkalotic, or basic.
We started our control group by adding 1mL of lactase (Lactase Drops, Seeking Health, Bellview, WA) to 2mL of milk (for lactose). We tested our solution for glucose by using a glucose strip (Precision Laboratories, Cottonwood, AZ), then set it aside for five minutes. By adding 1mL of NaOH to 9mL of lactase, we created our experimental group. We took 1mL of the solution and added it to 2mL of milk. Next, we used a glucose strip, as we did with the control group, dipped it into the solution and let it sit for five minutes. After the five minutes was up, we found our results to be negative, with 0mg of glucose present in the solution.
Results
The results from our control group and experimental group were completely different, as we predicted in our hypothesis. Our results from our control group came back positive, indicating that there was glucose...

Find Another Essay On How pH Affects an Enzyme's Ability to Catalyze - Honors Biology I - Lab Report

Grade 12 Biology Photosynthesis Lab - Bill Crothers Secondary School, Biology - Lab Report

1704 words - 7 pages electron acceptor, NADP+ gets reduced (gaining e- from the last protein) with a H+ from the stroma into NADPH (which lowers [H+] in stroma). Due to the electrochemical gradient, H+ are forced through ATP synthase, which want to reach equilibrium with H+ in the stroma, and this powers ATP synthase to catalyze the reaction between ADP and an inorganic phosphate to create ATP. The products of the light reactions are used in the calvin cycle to produce

How the base area of a paper boat affects how much mass it can carry - Science - Lab report

2279 words - 10 pages An investigation of how the base area of a paper boat affects how much mass it can carry without sinking Introduction: Buoyancy is very important in all of our lives. It determines whether an object will float or sink, which is vital to the design of boats, life preservers and many more floating objects. A flaw in the buoyancy of an object can lead to catastrophic events such as sunken boats, oil spills and more. When an object is

Chemical Lab on The Effects of pH and Various Other Substances on the Function of Peroxidase - AP Biology

1153 words - 5 pages return the pond to its normal pH of 7 to allow the papers to rise to the surface quicker. Hamdy Inusah AP Biology P6 10/3/16 Introduction: Enzymes are biological catalysts that accelerate, or catalyze, chemical reactions by lowering the amount of activation energy needed to get a reaction to occur. Enzymes are named primarily after the substances that it catalyzes the breakdown of, and they also tend to end in the letters -ase. So, in the

lab report for osmosis in potato strips - biology - repot

815 words - 4 pages Lab Report Template Title • make it brief, yet descriptive • introduce the relationship between independent variable and dependent variable • together with your name and number in the cover page Research question • What big question(s) are you trying to answer? • ex: How does the quality of the air we breathe affect cognitive function in 8th grade bio students? Introduction • background information about the theme of the lab • ex: The

AP Biology Lab Report for Population Genetics and Microbiology

2632 words - 11 pages best in today to be most informed with current issues, so not only was the lab an opportunity for students to understand microevolution and population genetics, but it also helped them to understand the argument for evolution when considering genetic variation causes.Sources CitedPearson Education, Inc. Campbell Biology. © 2002. Date of Access: 01/18/2005. URL: www.biology.com/UVa (University of Virginia). Hardy Weinberg Equation of

Biology Lab Report on the Effects of Photosynthesis

1275 words - 5 pages , Lugol's iodine solution in dropper bottles, 250-mL beakers, 100-mL beakers, tongs, foreceps, Petri dishes, glass-marking pencil, 70% alcoholMethodPART I - Effects Of Light And Dark On Starch FormationThe leaves used for Part I are completely green. A few of these leaves have been entirely and partially covered with black paper two days before the lab. Then the plant was exposed to good light during the day.1. With a glass-marking pencil, label one

This is a short report about the dissufion of carbon dioxide on the pH of water. - BGHS - Biology - Report

423 words - 2 pages Perform an experiment to demonstrate the effect of dissolved carbon dioxide on the PH of water. AIM: To demonstrate the effect of dissolved carbon dioxide on the PH of water HYPOTHESIS: Dissolved carbon dioxide in water affects the PH level. Therefore dissolved (co2) decreases the PH of water EQUIPMENT NEEDED: · PH probe · Data logger · 250ML beaker · Distilled water · Universal indicator · Limewater · Straw HYPOTHESIS: When carbon dioxide is

Lab Report for Group I Cations

798 words - 3 pages laboratory cabinets and used in this week's experimentations.Refer to Figure 9.1 on Page 94 of the lab manual for the experimental procedure (DePaul University, 94). This flowchart was followed for the known sample. The flowchart was then repeated for the unknown sample. Instead of using NH3, NH4OH was used. Precisely fifteen drops of deionized water were added in the first step of the right-hand side of the flowchart. On the left-hand side of

Enzyme Kinetics Lab Report: The reaction rate of enzyme, '-amylase in Starch-Iodine solution at different temperatures and pH levels

1996 words - 8 pages made a hypothesis that under the condition of temperature at 40'' and pH at around 4, the enzyme works at best efficiency. The result was little bit off but quite similar to what I expected from my hypothesis. The first two graphs (Figure1, Figure2) shows obvious decrease in absorbance as the times increases. The rest of two other graphs (Figure3, Figure4) show how the reaction rates are different at various temperatures and pH levels. Within the

Biology Lab Report On The Effects Of Adrenalin On A Daphnia

1450 words - 6 pages concentration, there must have been some threshold which was over come, because the heart rate rose noticeably from about 152 and 205 to 280; this is a clear positive change in heart beat.Questions or Discussion1. How would you expect adrenalin to affect daphnia? - It will probably have no effect with the 0.001% concentration of adrenalin, but I expect to already see a increase in heart rate by maybe a 0.01% adrenalin concentration. This will only be

Biology Lab Report on the extraction of Chlorophyl from Plant Leaves

869 words - 3 pages pigment types are present in a green leaf?It is hoped to be able to identify the four different pigments types of a leaf. As the filter paper with the solvent will separate the pigments in terms of solubility, a clear segmentation of each is expected to show off.As various chemicals were used in the whole process of this lab, certain variables might have influenced the results in terms of the purity of each chemical or purity of the used

Similar Essays

Investigate How Ph Affects The Ability Of Raw Meat To Absorb Water

548 words - 2 pages Investigate how PH Affects the Ability of Raw Meat to Absorb Water I am planning an experiment to investigate how PH affects the ability of raw meat to absorb water. · Independent Variable The independent variable for this experiment is the PH of the solution the steak is marinated in. I will achieve a range of different PH values by using buffers set at PH 1, 3, 5, 7, 9. I predict that there will be an optimum PH where the steak will absorb

Enzymes Lab Report Biology 1406 Biology Enzymes Lab Report

797 words - 4 pages mg/Dl 0 mg/Dl *Deviated from expected results, which should have been 0 mg/Dl (flaw explained in conclusion). Conclusion The disaccharides lactose and sucrose will naturally dissociate into glucose and galactose, and glucose and fructose, respectively, when water is introduced. These reactions, however, will take place over a rather long period of time without the presence of an enzyme to catalyze said reactions. This lab investigated whether

The Effect Of A Cell's Size And Its Ability To Diffuse Materials Sbi4 Ue Biology Lab

2068 words - 9 pages frequently divide to form two new smaller cells. Using the information you obtained in this lab, explain why individual cells tend not to grow very large? As cells grow larger, their surface area to volume ratio would begin to decline, meaning an inefficiency in the cell’s ability to carry out its processes as it would be harder for particles to move past the cell membrane rapidly. Diffusion is a very important process for cells, as it transports

To Test The Effects Of Antacids On Pepsin's Ability To Digest Protein. Biology Report Minus Introduction, Discussion & Conclusion

1808 words - 7 pages with me, even when I didn't know how to peel an egg· Ms Murray - for going to all extremes to find the materials for my experiment· Fellow students - for lending me their cameras in the absence of my own· Big brother - for teaching me how to calculate percentages· Tim Rossow and Daniel Bradford - for explaining what my brother could not· Google - for once again, being a reliable and trustworthy search engine&middot