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I Am Going To Investigate How The Temperature Affects The Catalyse Enzyme On Hydrogen Peroxide, Using Potato Discs For The Catalyse.

1552 words - 6 pages

AimI am going to investigate how the temperature affects the catalyse enzyme on hydrogen peroxide, using potato discs for the catalyse.HypothesisCatalyse is a biological enzyme which works best between 30 and 40 degrees, like most enzymes. It also has an optimum pH.Catalyse breaks down Hydrogen Peroxide into water and oxygen. Plant and animal cells contain catalyse.`I predict that the catalyse enzyme found in the potato will break down the substrate (Hydrogen Peroxide) quickest at 37- 40 degrees Celsius because enzymes work best at certain temperatures, usually body temperature. If the temperature is above 40 degrees the enzyme will become denatured so molecules of Hydrogen Peroxide won't be able to fit into it and so they can't form an enzyme substrate complex and can't be broken down, so no oxygen or water is created. If it is below 37 degrees the amount of activity is reduced, as temperature is a limiting factor in this part of the experiment.I predict that the graph for the total oxygen evolved will look like this:I think this because between A and B the rate of reaction increases the molecules of Hydrogen Peroxide will have more kinetic energy and so there will be more collisions, as they move around the container and hit each other. More enzyme substrate complexes will be formed and so more oxygen and water is formed.At point B the temperature is the optimum.Between points B and C the temperature is too high for the enzyme to work and the shape of the active site is damaged. The reaction slows down, as the enzyme is eventually denatured.EquipmentStop clock10ml measuring cylinderDelivery tubesBungGooglesHydrogen peroxide (...ml each time)Potato discs (40 1mm thick)ControlsAmount of hydrogen peroxide (20ml)Length of experiment (3min)Time between measurements (30sec) - same person timingEquipment usedTemperatureMass / Size / Surface area - using precision to cut parts of the potato.VariablesVariables are things that I can change. The only possible independent variables from this investigation is:Temperature - the rate of reaction depends on the temperature as enzymes work best at certain temperatures, and I will measure the rate of reaction at different temperatures.SafetyI will keep my safety goggles on at all times to prevent painful eye injury,Handle hydrogen peroxide with as it is corrosive,Keep all apparatus away from the edge of the desk to prevent breakages,Do not leave bags lying around on the floor in the way of a pathway,Do not run around or rush about.Results32°c42°c52°c62°c30 seconds11.265.51 minute23.19.561 min 30 secs35.411.57.52 minutes5.561282 min 30 secs6712.58.53 minutes6.5812.99By varying the temperature the enzyme will react differently to each temperature this is due to the collision theory, the collision theory is when lots of collisions are made the rate of reaction will be higher than when the is relatively few collisions.AnalysisI have drawn two graphs, one showing the total of oxygen evolved at each...

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