This website uses cookies to ensure you have the best experience. Learn more

Identification Of Unknown #79 Essay

712 words - 3 pages

The objective of this lab was to identify unknown bacteria culture by using various differential tests. There are many reasons for knowing the identity of microorganisms including to find the correct antibiotic to treat infections the bacteria may have caused. All the methods and techniques used to identify unknown bacterium #79 was learned in the microbiology laboratory.
Test Result
Unknown# 79 was grown on TSA slant in a 370 c temperature after two days, the microbe replicated to more than 100 microorganisms, which has cream pigments, and convex elevations. The microbe entire margins and convex elevations. The microbe appears to be circular and abut 0.4-0.7 micro meter in ...view middle of the document...

The purpose is to see if the microbe can use the protein gelatin as a source of carbon and energy for growth. Use of gelatin is accomplished by the enzyme gelatins. A medium containing gelatin is used. We have all seen how gelatin forms a semisolid substance. In the medium it serves as the solidifying agent in addition to its role as a source for carbon and energy. When gelatin is used, the medium changes from semisolid to liquid and cannot be solidified. (Gelatin Hydrolysis Test) In this test the medium flows when the tube is tilted, which shows that the gelatin been digested. Unknown microorganisms use gelatin as a source of carbon and energy for growth but didn’t use starch. Urea digestion, iodole production, and H2S Production shows negative result in unknown bacteria. Unknown #79 had catalase, a protective enzyme capable of destroying the dangerous chemical hydrogen peroxide. Unknown #79 was identified Staphyloccus aureus by doing several tests as shown in Table below.
Table 1.Characteristics of Staphyloccus aureus
Characteristics Stephyloccus aureus Unknown #79
Gram reaction + +
Cell morphology coccus coccus
Cell arrangement cluster cluster
Motility - -
Pigmentation + yellow
Optimal growth temperature 37ºC...

Find Another Essay On Identification of unknown #79

Chemistry lab report

1106 words - 4 pages The goal of the project was to characterize an "unknown" organic acid in order to make a proper identification of the acid, while learning proper techniques for scientific measurement and analysis of error. In order to ensure the most accurate data, a purification was performed by the process of recrystallization. To perform the recrystallization the powder was dissolved in a minimal amount of hot ethanol/H2O solvent that allowed the unknown

Chroatography Essay

440 words - 2 pages acids are organic compounds that combine to form proteins.Chromatography: is the collective term for a set of laboratory techniques for the separation of mixtures.Representative fraction: The ratio of each element in the mixture solution.DiscussionI am confident about our identification of the amino acids. Because the Rf of same amino acid in the unknown are very close the result of our measurement to Rf of known amino acid. And some amino acid

The Controversy Over Voter Identification Laws

2213 words - 9 pages In 2002, with the intention of updating election technology, hampering voter fraud, and increasing faith in the electoral system, President George W. Bush signed into law the Help America Vote Act (Gerken 14). Since the implementation of this act, controversial voter identification laws have begun springing up all over the country. According to the National Conference of State Legislatures, “thirty-one states require all voters to show ID

biological profile

978 words - 4 pages excavation skills. Most elements show signs of root etching, varying in visibility. This erosion varies in severity throughout all of the elements. The left and right femur and the left tibia are peeling due to an unknown cause. Works Cited Trotter, M., Gleser G.C. 1958. A Re-Evaluation of Estimation of Stature Based on Measurements of Stature Taken During Life and of Long Bones After Death. American Journal of Anthropology 16 (1): 79-123

Identifying Two Unknown Species of Bacteria

1353 words - 5 pages Identifying Two Unknown Species of Bacteria Materials and Methods Week 1, Day 1 (10 November 2000) The first day an unknown sample was assigned to each group of students. The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The morphology of the two types of bacteria was viewed under the microscope and recorded. Then the sample was put on agar plates using the quadrant streak method for

Applying the Methyl Red (MR) Test

1196 words - 5 pages The MR test media contains peptone, glucose, and a phosphate buffer (Stout et al, 45). To perform the MR test, I used the stabbing technique to inoculate the MR media. I sterilized the stabbing utensil under an open flame, obtained a small amount of unknown bacteria, and stabbed the MR media. Once the MR media was inoculated, I let it incubate for 24 hours in the 37°C hot room. After the 24-hour incubation, I added 15 drops of Methyl Red to the

Identifying an Unknown Microbial Organism

1704 words - 7 pages Identifying an Unknown Microbial Organism Introduction The purpose of identifying an unknown microbial agent is so that the five “I’s” of Microbiology, which are, Inoculation, Incubation, Isolation, Inspection and Identification can be practiced. Providing an unknown microbial agent tests the ability of ones skill of the above techniques and to accurately obtain the correct results and compare these results to biochemical test

ID Cards

1015 words - 4 pages credit cards they are small and have importance with everyday activities. People go about scanning their way in and out of work or using them for identification. The ID card can be easily slipped into a pocket or placed in a bag, but once it is in there they can never find it again. Its compact form allows the card to hide in those places unknown to a person and its flexibility allows it to move with the object so they cannot find the card no matter


1219 words - 5 pages the development of the disorders is their life situations (Flory et al., 2002). There is a possible link between an early lack of empathy, understanding the perspectives and problems of others and this includes other children and the later onset of the disorder. What may improve the long term outcome is early identification of these personality problems. The precise causation of an Antisocial Personality Disorder is unknown but certain factors is

Bacterial Species

2319 words - 10 pages that unknown #52 was Staphylococcus aureus. When conducting these various test, we accumulated the results to be accurate and consistent with the identification of the bacterial genus. When viewing the flow chart in the next page, there no significant error in which my unknown would have not been questioned if it was wrong. The only test that I did extra to further solidify my unknown was the Bacitracin test which gave us a positive result that identified that our result is Staphylococcus aureus. The precise precaution was taken and multiple tests were done to verify the accuracy of unknown #52, which undoubtedly gave us the correct unknown name Staphylococcus aureus.

Unknown Lab Report

958 words - 4 pages gained from the sessions in the laboratory, we can now integrate what we have learned to the process of finding out the unknowns given. Materials and Methods The professor gave out the unknown specimens. It contained one-­‐gram positive and one-­‐gram negative bacteria from the given list. I was assigned unknown A. The process of identification was achieved by utilizing procedures learnt during the semester. Procedures were

Similar Essays

An Investigation Into The Identification, Properties, And Synthesis Of An Unknown Ionic Compound

1186 words - 5 pages Abstract This paper describes the methods used in the identification, investigation of properties, and synthesis of an unknown compound. The compound was identified as calcium nitrate by a variety of tests. When the compound was received, it was already known to be one of twelve possible ionic compounds. The flame test identified the presence of the calcium anion in the compound. The compound tested positive for the nitrate cation using the

Types Of Poject Management Essay

1011 words - 5 pages opportunities management (Ward and Chapman, 2003). Uncertainty management gives out neither positive nor negative meaning. It is just simply a probability of unknown event might happen. Thus, this might help with the early identification process of uncertainty. It is easier as the management will identify all the uncertainties then collects enough information (known-unknown and unknown-unknown) before labeling where the uncertainties belong either

Cyalume Essay

1222 words - 5 pages are very inaccurate. So many errors were made.Table 2 Benzoic Acid 0.658 mp Benzoic Acid 119 p-nitroaniline mp p-nitroaniline 146 biphenyl mp biphenyl 79 Discussion the three compound was already mixed and the first isolation was benzoic acid Extraction is a delicate process. The.Conclusion The purpose of extraction is to determine the identity of an unknown liquid dissolved in solution. Through a series of steps and adding

Oklahoma Fish Kill Study: Looking For A Toxic Needle In An Environmental Haystack

2007 words - 9 pages chlorin-e6-trimethyl ester (Figure 1), MW 638.31043 Da, C37H42N4O6. In order to be indisputably certain that this was the correct identification, a standard of chlorin-e6-trimethyl ester was obtained from Frontier Scientific (Logan, Utah). Using the collision induced dissociation (CID) function of the LC-ITMS, a CID mass spectra of the standard was obtained and compared to the unknown spectra detected at mass m/z 639.4 Da (M+H)+ in fish kill