This website uses cookies to ensure you have the best experience. Learn more

In Vitro Propagation And Assessment Of Clonal Fidelity Of Dendrobium Aphyllum

1609 words - 6 pages

Orchids belong to the family Orchidaceae one of the largest and most diverse groups among angiosperms. According to one estimate, the family Orchidaceae includes 800 genera and 25,000 species (Stewart and Griffiths 1995). These plants are outstanding in many ways as they have diverse shapes, forms and colors. However, due to heavy rate of deforestation and other anthropogenic pressures the natural populations of many splendid orchids are depleting fast from their natural habitats at an alarming rate and their conservation is becoming a matter of global concern. Presently, all the orchid species are indexed in the Red Data Book of International Union of Conservation of Nature and Natural Resouces (IUCN). Also, the family Orchidaceae is now included in Appendix-II of Convention of International Trade in Endangered Species of Wild Fauna and Flora (CITES) (Senthilkumar 2001).
The use medicinal use of orchids in treatment of disorders and ailments dates back many centuries and was reported by the Chinese (Bulpitt 2005). The Chinese pharmacopoeia, “The Sang Nung Pen Tsao Ching’’, illustrated Dendrobium as a source of tonic, astringent, analgesic, and anti-inflammatory compounds as far back as 200 BC and since vedic times (Singh and Tiwari 2007). Orchids are used in traditional medicine as they are rich in active compounds including several alkaloids (Okamoto et al. 1966; Lawler and Slaytor 1969; Elander et al. 1973; Nurhayati et al. 2009). Being one of the main constituents of “Herba Dendrobii”, Dendrobium aphyllum deserves special mention. The phenolic constituents of D. aphyllum comprised nine bibenzyls and two benzylethanyl compounds of which moscatilin and moscatin are also present (Ng et al. 2012). The paste of D. aphyllum is used in the treatment of organ deformities (Hossain 2009). Apart from its medicinal use, its flowers are also very attractive. Thus as this orchid is having a broad spectrum usage, it’s subjected to overexploitation and the wild populations of this splendid orchid is vanishing at an alarming rate. To prevent this beautiful orchid from extinction, it is necessary to propagate and reintroduce them in natural habitats.
Conventionally, Dendrobes are propagated propagated through cuttings, separation of offshoots and keikis produced from the old stems but only a few plants are formed after 2–3 years. But in the present scenario, where the habitats of these orchids are under severe threat, conventional propagation methodologies will not be able to cater the present issues. Tissue culture techniques using various explants have been widely used for in vitro mass propagation of several commercially important orchids over the past few decades (Dohling et al. 2012). In D. aphyllum the reports of in-vitro propagation are very less (Nayak et al. 1997; Talukdar 2001; Hossain et al. 2012). Moreover, only shoot bud production from nodal explant culture has been reported in this species. For large scale propagation a simple, economical,...

Find Another Essay On In Vitro Propagation and Assessment of Clonal Fidelity of Dendrobium Aphyllum

Short-term in vitro Inhibition of Glycogen

1578 words - 6 pages activity increased the uptake of glucose into the cell and the activation of IRS-1 by tyrosine phosphorylation. 1. American Diabetes Association 2. Role of Glycogen Synthase Kinase-3 in Insulin Resistance and Type 2 Diabetes. Erik J. Henriksen and Betsy B. Dokken 3. Short-term in vitro inhibition of glycogen synthase 3 potentiates insulin signaling in type I skeletal muscle of Zucker Diabetic Fatty rats. Erik J

Literature Review of In Vitro Fertilization

2030 words - 8 pages vitro fertilization (the fertilization of an egg cell outside the body) has begun to see an increase in viability as questions about its ethics and morality have become more accepting due to the costs of a procedure being cut down (not covered by the Affordable Care Act), developments in medical and scientific technology through IVF, and subsidiary factors: the advent of “designer babies” and the increasing tolerance of homosexuality—leading to

The Hidden Dangers of In Vitro Fertilization

701 words - 3 pages The Hidden Dangers of In Vitro Fertilization   In Vitro Fertilization (IVF)involves giving a woman drugs to induce ovulation and then harvesting (collecting) those mature eggs through a laparoscope. The man masturbates to collect the semen. Then, the semen and ova are mixed in a dish, where fertilization may occur. Then the tiny, new embryonic human(s) are put into the cavity of the woman's uterus with the hope that they will plant and

Feminist Analysis of In Vitro Fertilization

2791 words - 11 pages sometimes I was nauseous and I was very stressed." (Mason, 2003, p.2)History of DevelopmentIn vitro "in glass" fertilization began in Great Britain in the 1970's with Robert Edwards, a Ph.D. physiologist and Patrick Steptoe, a gynecologist. In the 1960's, Dr. Edwards experimented with human ovaries removed at surgery. In 1967, he was the first to fertilize a human egg outside of the body. At the same time, Steptoe was developing a technique called

Transfer of Multiple Babies: In Vitro

1670 words - 7 pages Embryo transfer can happen in many different animals including humans. Many people do not know that most farmers do embryo transfer because they use their dominant genetics in order to produce dominant offspring. Embryo transfer is not only better on the economic side such as costs of transferring eggs from cow to cow but the offspring you get from the egg will improve your herd and bring in more profit. Some transfers of preimplantation

Effects of paring and macro-propagation on the performance of three cultivars of plantain propagules

1345 words - 6 pages techniques exist for the control of diseases and soil borne pests in the plantain planting materials.These range from paring, heat treatment to the use of pesticides (nematicides) with a wide range of costs, labour input and environmental risks.There has been no study on the effect of paring and macro-propagation on the performance of plantain propagules. This paper presents data from an investigation on the performance of three cultivars of

Implementation Of Information Retrieval (IR) In An Electronic Commerce Architecture Using Back Propagation Network Learning Algorithm

2213 words - 9 pages Implementation of Information Retrieval (IR) in an Electronic Commerce Architecture using Back propagation Network Learning Algorithm Dr. Riktesh Srivastava Associate Professor, Information Systems Skyline University College, Sharjah, UAE rsrivastava@skylineuniversity.ac.ae Abstract The present study assesses the technique of back propagation neural networks to appraise the required Information Retrieval (IR) and thereby decreasing the average

A randomized study on the outcomes of gonadotropin priming and nonpriming in in vitro maturation

2042 words - 9 pages and the reasonable pregnancy rate2, 3, in vitro maturation of oocytes (IVM) has become an alternative treatment of in vitro fertilization (IVF), especially for the women who are infertile due to PCOS. Successful oocyte maturation includes complete maturation of three compartments, namely meiotic, cytoplasmic and molecular4, which are crucial for good embryo development. Gonadotropins that produced from pituitary are indispensable for oocyte

In Vitro Fertilization: Ethical Problems of Mitochondrial DNA and Three Biological Parents

904 words - 4 pages In vitro fertilisation: ethical problems of mitochondrial DNA and three biological parents Arttu Mäntylä, Bioethics course 2013 Mitochondria are essential for the cell energy production through the citric acid cycle. In order for the cycle to work in a best way possible, the mitochondria are equiped with their own DNA that primarily codes for proteins vital to the energy production and oxidative metabolism of the cells. Mitochondrial DNA has

Synthesis, DNA binding and in-vitro cytotoxicity studies of novel bis-pyrazoles

1785 words - 8 pages pancreatic cell line. (Insert Table 2 here) 3. Conclusion In summary, a series of novel bis-pyrazoles with amides, sulfonamides (6a-o) and ureas (6p-t) were synthesized and subjected to DNA binding studies. The binding pattern was analyzed through an AUTODOCK server in that 6h showed lowest conformational energy than other compounds. Then all the above compounds were evaluated for their in vitro cytotoxicity against Panc-1 (human pancreatic

In Vitro Fertilization and Infertitlity

1488 words - 6 pages Rheanna C. Robinson Dr. Moody Honors Biology/ Period 7 16 May, 2014 In Vitro Fertilization and Infertility “In Vitro Fertilization or artificial fertilization is the fertilization of an ovum by sperm outside the body when normal conception is not achievement because of a woman’s low fertility” (http://www.medterhttp://history1900s.about.com/od/medicaladvancesissues/a/testtubebaby.htmms.com/script/main/art.asp?articlekey=7298). In virto is a

Similar Essays

Stages Of In Vitro Ferlization Essay

1276 words - 6 pages In Vitro Fertilization (IVF) is a procedure in which an egg (oocyte) from a woman’s ovaries is removed and separately fertilized with male sperm in a laboratory before being implanted into the woman’s fallopian tubes. 1 2 The first stage of IVF is called superovulation. Follicles are structures within which the oocyte develops, in order to retrieve multiple follicles several hormone and drug combinations are administered. Consequently, the ovary

Evaluation Of In Vitro And In Vivo Osteogenic Differentiation

1355 words - 5 pages + hUCMSCs, (D) nHA/CS/PLGA + hUCMSCs, (E) MTT assay - Effects of the scaffolds on cell adhesion and proliferation, (F-I) The expression of cell activity after three weeks of culture Osteoid tissue was found in the greatest quantities in scaffolds with nHA/CS/PLGA present. The presence of this tissue is directly correlated to the amount of osteogenic cells present and formed in vitro. It is thought that the increase in osteoid tissue and osteogenic

Fidelity, Integrity And Bravery: A Brief History Of The Fbi

1785 words - 7 pages with this act. During the next few years the bureau grew to more than 300 agents and 300 support staff. The 1920’s brought some peculiar challenges to the bureau. Prohibition was in full blast, and the inexperience of all law enforcement agencies only made it worse. Gangsterism was rampant, and public contempt for prohibition made matters almost unbearable. Characters like Alphonse Capone, Lucky Luciano et al. germinated from this situation

Full Explanation Of Plant Propagation Methods

1372 words - 5 pages PLANT PROPAGATIONReproduction:SexualSexual reproduction in plants uses seeds and spores. And consists of the fusion of male and female gametes to produce and offspring which is not genetically identical to the parent.Asexual reproduction is from budding off the parent to produce offspring genetically identical to the parentMethods of Vegetative Propagation:CuttingsPlant cutting, also known as striking or cloning, is a technique used for