Intracellular Localization Of Respiration And Glycolysis In Sarcophaga Bullata

1678 words - 7 pages

Intracellular Localization of Glycolysis and Respiration investigates which part of the Sarcophaga bullata cells carries out glycolysis and respiration. Sacrophaga ba. mitochondria and cytoplasm from thoraces cell were used to form a homogenate, supernatant, and pellet. Mannitol, buffer mix, glucose, succinate, homogenate, supernatant, and pellet were added to seven different test tubes to create different solutions. The test tubes were then observed to see which ones bleached after the homogenate, supernatant, and the pellet were added to the solutions and mixed. The bleaching of the test tubes showed where oxygen was used. Test tubes one, two, four and seven bleached. The reason the test tubes bleached was because they contained glucose which is used to carry out glycolysis. These test tubes also contained succinate and pellet which allows respiration to occur. Test tube seven did not have glucose added to it, but the fly was fed sugar water, causing the fly have glucose in its body form the sugar water, which showed that respiration occurred. Test tube four contained the supernatant and the pellet together which is used for glycolysis and respiration. The data collected did not match the data of other groups in the lab class. The difference in the data was caused by student error which could have been done by not adding a certain ingredient.

Glycolysis is the conversion of two pyruvates into a 3-carbon molecules. The conversion is a metabolic pathway. Two adenosine tri-phosphate (ATP) molecules are produced for each glucose molecule. Two phosphates are added to the glucose molecule and sliced to the two pyruvates to create glyceraldehyde 3-phosphate, known as G3P (Lange 2011). The G3P oxidized and electrons are transferred to nicotinamide adenosine dinuleotide (NAD) to produce nicotinamide adenine dinucleotide phosphate (NADPH). This transfer of electrons occurs four more times to produce the final product of two pyruvate molecules. No oxygen is needed for glycolysis to occur. If no oxygen is present, the NADH passes the electrons to an organic molecule. If oxygen is present, the electrons of NADH are passed to the electron transport chain (Lange 2011).
Cellular respiration is known as the complete oxidation of glucose. In the Krebs cycle pyruvate is broken down into NADH. NADH is reduced NADPH. The electrons are then moved into the electron transport system (ETS) to gain energy (Lange 2011). ATP is synthesized to power the cell's activities. The substrate-level phosphorylation transfers phosphate to ADP and generates ATP, which is powered by a protein gradient. Respiration occurs in the mitochondria and oxygen is needed (Lange 2011).
Sarcophaga bullata, are found in the United States and southern Canada to Quebec. Flesh flies feed on carrion, fecal matter, and garbage, and are harmless to humans. Flesh flies go through four stages of metamorphosis (Ward's Natural Sciences). Metamorphosis consists of the egg, the larvae, the...

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