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Investigate The Effect Of Temperature Change On The Rate Of Hydrolysis

479 words - 2 pages

Investigate the effect of temperature change on the rate of hydrolysis
of starch using the enzyme amylase.

Amylase investigation
---------------------

AIM: To investigate the effect of temperature change on the rate of
hydrolysis of starch using the enzyme amylase.

These are variables that may affect the rate of the reaction:

* concentration of amylase

* concentration of iodine

* temperature conditions of reaction

* pH conditions of reaction

I intend to investigate the effect of temperature change on the rate
of the hydrolysis of starch, using the enzyme amylase.

In order to do this while also ensuring a fair test, I shall maintain
all other latent variables constant.

PREDICTIONS: I expect that the time taken to evolve a set volume of
gas will decrease as the concentration increases. This, in other
words, means that to increase the concentration, will increase the
rate of the reaction.

I would expect that if the concentration is doubled, so too is the
rate of the reaction:

Rate of reaction µ concentration

JUSTIFICATION: Enzyme molecules have a very specific method of
converting those molecules upon which they work (the substrate
molecules).

Enzyme molecules are proteins that act as biological catalysts, so
they are not themselves used up when converting substrate molecules.
On every enzyme molecule is an active site where the substrate
molecules are joined to the enzyme molecule, and converted to produce
molecules.

Every enzyme is specifically shaped to fit the substrate upon which it
works, and therefore they do not work in conditions that denature the
molecules (e.g. very high temperatures).

The graph shows how rates are affected by substrate and enzyme
...

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