Isolation And Identification Of Thermophilic Fungi

1158 words - 5 pages

We isolated thirty thermophilic fungi with ability to grow at 45 °C on medium containing wheat bran and casein as carbon sources and produced proteases in both SSF and SmF (data not shown). The fungus strain F.2.1.4 presented the highest protease activity and was sub-jected to taxonomic characterization.
Analyses of macro and micro-morphological characteristics accommodated the isolate F.2.1.4 in the genus Myceliophthora due to the presence of spreading colonies with dense aerial mycelium and blastic conidia often borne in ampulliform swellings (Van Oorschot, 1980). Particularly, this isolate produced pulverulent pale mycelium, obovoid conida measuring 5.65-7.91 x 3.39-4.52 μm as well as conidia walls finely ornamented. Based on such char-acters the isolate F.2.1.4 is similar to Myceliophthora thermophila, however, some slight morphological differences were observed, for instance, M. thermophila has pale brown, woolly colonies and a variable range of conidia sizes 4.5-11 x 3.0-4.5 µm (Van Oorschot, 1977).
Data derived from BLAST (ITS-rDNA region) showed that the isolate F.2.1.4 had high sequence similarities (99-96%) with three unidentified Myceliophthora spp. isolated from geothermal soils in China; and 95-94% of sequence similarities with different strains of the Ascomycota genus Corynascus (one of the Myceliophthora teleomorphic states), includ-ing three new species: C. verrucosus sp. nov., C. similis sp. nov., C. sepedonium, C. sexualis sp. nov. (Stchigel et al., 2000). The phylogenetic tree showed that the isolate F.2.1.4 clustered with the three Myceliophthora strains from China (Figure 1).
Molecular and phylogenetic results corroborate the data from morphological analyses and considering the combined methods used for fungus identification, the thermophilic isolate F.2.1.4 was identified as Myceliophthora sp.. Species on this genus are commonly found in soil, molding open-stack alfalfa ensilage, fir and spruce pulpwood chips and wheat straw compost (Van Oorschot, 1980). Moreover, results derived from conventional, molecular and phylogenetic analyses suggest that isolate F.2.1.4 likely represent a new species in the genus Myceliophthora. Additional taxonomic analyses will be performed in order to confirm the new species hypothesis.
Enzyme production in solid-state (SSF) and in submerged (SmF) fermentations
Figure 2 shows the profile of protease production by Myceliophthora sp. in SSF and SmF. The maximum enzyme production was obtained in the third fermentation day in SSF (1.78 U mL-1) and in the forth day in SmF (0.38 U mL-1). The higher protease production achieved in SSF corroborate data reported by several investigators and reinforce the hypothesis that production of extracellular enzymes is higher in SSF since the conditions of this fermentation system are similar to natural fungal growth media (Aguilar et al., 2004; Silva et al., 2007; Morita et al., 1999; Ramesh and Lonsane, 1991). The data also showed that wheat bran, that...

Find Another Essay On Isolation and Identification of Thermophilic Fungi

Identification of Gifted and Talented Students

2013 words - 8 pages Identification of Gifted and Talented Students When I was in the second grade, all of the students at my elementary school were given a special test one day in class. We were told that it was not for a grade, but that it was to help the school know more about us. None of us really understood what the test was, or what the school would use it for, and it is certain that no one in my class that day understood the implications of what those

The Identification and Treatment of OCD

1305 words - 5 pages There are many different aspects to the identification and treatment of OCD and this paper attempts to identify classic symptoms and theorized risk factors/causes. In addition it explores the viable treatment options and evaluates the efficiency of each in alleviating symptoms while discussing how each treatment functions to facilitate recovery. Furthermore, it assesses the extent to which the treatments may be used together to maximize

Loss and Isolation Themes of "Frankenstein"

1010 words - 4 pages Isolation is a common human tendency that society uses to deal with a stressful situation. In the timeless novel "Frankenstein", by Mary Shelly, there is a constant theme of isolation, leading to loss and tragedy. Consequently, this theme becomes exponential as the loss and tragedy lead to more isolation. The cycle continues throughout the story, up to the point where both Victor Frankenstein and his creature die near the North Pole. The

Frankenstein- The Effects of Isolation and Rejection

741 words - 3 pages The Effect of Isolation and Rejection In the novel Frankenstein by Mary Shelley, the monster is born more or less with the mind of a baby. He craves attention, love and nurturing as all babies do. The monster was left with no one to teach him anything, and to understand the world solely on his own. After observing, and slowly figuring out how the world works, he was unable to imitate because no one accepted him, including his creator

Solitude and Isolation of Hawthorne and Young Goodman Brown

2101 words - 8 pages           In the Nathaniel Hawthorne tale, “Young Goodman Brown,” we see and feel the solitude/isolation of the protagonist, Goodman. Is this solitude not a reflection of the very life of the author? At the very outset of the tale we see a purposeful secretiveness if not outright deception by Goodman Brown when his wife of three months pleads with him to stay home on this particular night: "Dearest heart," whispered she, softly and

Of mice and men the loneliness and isolation

3339 words - 13 pages Untitled Of Mice and Men Loneliness and isolation Loneliness and isolation is a big issue in this book and I am here to talk about it. Many characters have been affected by a person or a group and became independent or very lonely. These characters want others to care for them and talk to them or even befriend them and make them feel more at ease. The people that I want to focus on and go deeper into explaining their thoughts

Of Mice and Men Essay: The Loneliness and Isolation

3144 words - 13 pages Of Mice and MenLoneliness and isolationLoneliness and isolation is a big issue in this book and I am here to talk about it. Many characters have been affected by a person or a group and became independent or very lonely. These characters want others to care for them and talk to them or even befriend them and make them feel more at ease. The people that I want to focus on and go deeper into explaining their thoughts and actions are: Curley's wife

Isolation Comparison between Heart of Darkness and Frankenstein

1882 words - 8 pages Humans are naturally social and interactive. Occasionally, a person will want or need to be away from others, which are very natural (Good Therapy Organization). However, prolonged isolation is not such a good thing, in fact, it can be downright harmful. In fact, isolation for extended periods of time can be considered a risk factor. Isolation can be categorized with smoking and obesity in terms of how damaging it is to the human body, as

Isolation and Nature in the Works of Robert Frost

3441 words - 14 pages Isolation and Nature in the Works of Robert Frost During the height of Robert Frost’s popularity, he was a well-loved poet who’s natural- and simple-seeming verse drew people - academics, artists, ordinary people both male and female - together into lecture halls and at poetry readings across the country.1 An eloquent, witty, and, above all else, honest public speaker, Frost’s readings imbued his poetry with a charismatic resonance

Comparing Isolation of the Protagonist in The Trial and Nausea

669 words - 3 pages Isolation of the Protagonist in The Trial and Nausea   Kafka and Sartre provide effective settings for their novels by presenting their protagonists in isolated environments. Each character experiences very slight contact with other people, and the relationships they do have with the other characters exist at a superficial level. In The Trial, Joseph K. is placed on trial for an offense about which he is told nothing. As he attempts

The Isolation, Exploitation, and Discrimination of Border Crossing

1863 words - 7 pages , but they were faced with isolation, exploitation, and discrimination. Until the United States finds a proper solution to solve residential segregation, institutionalized racism, and the misinterpretation of others, these obstacles will continue to exist in the process of assimilation of immigrants into America.

Similar Essays

Features Of Bacteria, Viruses And Fungi

2883 words - 12 pages Features of Bacteria, Viruses and Fungi Infectious diseases are diseases or illnesses (caused by bacteria or viruses) that can be transmitted from person to person or from organism to organism by touch or the exchange of blood or saliva and also could be passed through air molecules. For instance, the common cold and AID's are infectious diseases, whereas illnesses such as diabetes and gout are non-infectious diseases

Importance Of Fungi And Their Use In Genomics

2553 words - 10 pages the genome of Saccharomyces cerevisiae (Goffeau et al. 1996).As a result the dawn of a new genre in human disease has blossomed. Related by Lee et al (Lee et al. 2002), this supposedly was the first dramatic large-scale study on the eukaryotic fungal diseases. This warming investigation led the way to genomics as now many researchers have mimicked Goffeau et al and have plowed out genes of fungi and their functions in pathogenesis, and human

This Is A Somewhat Brief Description Of Fungi, It's Reproductive System, And How It Obtains Nutrients.

705 words - 3 pages Hello, and welcome to the wonderful world of fungi, when most people think of a fungus they think of what? Mold, itchy feet, and that nasty green stuff on a month old sandwich. However these are only some of the characteristics of some types of fungi.First, we'll go into the several types of fungi that can prove to be helpful. The most widely accepted helpful fungi is Penecillium, this is the type they use in the common drug penicillin. Another

The Pros And Cons Of Ethnic Identification

832 words - 3 pages enriches societies and makes them multi-racial. Therefore, every ethnic group is essential to complete the mosaic. Although they are sometimes threatened, minorities must fight to preserve their principles. Why must they fight? Because racial identification has many positive impacts on the members. But it’s undeniable that this way is invaded by dangers. After evoking the positive and negative aspects of ethnic identification, we will suggest some