Re: Research Assistant
Dear Jason Geijskes,
Please accept my application for the above-mentioned position as advertised on the http://seek.com.au website on 5 May 2010. My interest in working with a leading agribusiness industry together with the job responsibilities you have mentioned inspired me to apply for this position. My qualifications and skills that I believe will be important for this position are briefly discussed bellow for your kind consideration.
Degree in biological science
Presently I am in the final stage of PhD and I will submit my thesis on 1 Jun 2010. I have been studying this degree at the University of Queensland, St Lucia, Brisbane, and the main focus of my research was to improve the drought performance of canola (Brassica napus) through molecular breeding. Through this research, I gained in depth knowledge in plant science including plant phenotyping (in field and glasshouse), genotyping and relevant techniques in molecular biology and genetics. Before this, I completed a Master of Applied Science (biotechnology) degree from The University of Western Sydney in 2004 which offered me knowledge and hands on experience in microbial fermentation technology.
Plant tissue culture/biotechnology
Tissue culture and experiments with transgenic plant is a routine exercise in the lab presently I am working. Often I performed tissue culture voluntary to help other researchers in our lab. I also did fungal tissue culture in a yearlong project conducted as a part of my master degree. In addition to that, I completed laboratory-based course on microbial fermentation where I operated bioreactors in experiments aiming to maximise microbial production of amylase (enzyme that breaks starch into sugar) in submerged fermentation.
Plant molecular characterisation
Cloning and molecular characterization constituted a major part of my PhD research. Here I cloned and sequenced genes in B. napus homologous to genes in Arabidopsis thaliana. I developed molecular markers from these gene sequences which in addition to other marker systems (including inter simple sequence repeats, intron length polymorphism) were used in molecular characterization of more than 150 canola genotypes.
Enzymology and protein characterisation
With a yearlong project in my master degree, I studied enzyme (catalase) kinetics (by spectrophotometric analysis) in response to the activation/inactivation of MAP (mitogen-activated protein) kinase pathway of a plant pathogenic fungus (Botrytis cinerea). I am also familiar with different protein purification and characterisation...