Micro Ribonucleic Acid Analysis

709 words - 3 pages

Studies are being carried out using animal models such as mice and zebra fish to understand the importance of micro RNA in vascular development. Knockdown of micro RNA 126 in zebra fish resulted in loss of vascular integrity and haemorrhage during embryonic development (65). Overexpression of micro RNA 92a (66) induced severe defects in inter segmental vessel formation in zebra fish. An independent study reported that deletion of micro RNA 126 resulted in 50 percent of mice being killed.
Micro RNA 23, 24, 27 are highly present in endothelial cells and vascularized tissues. Knockdown of micro RNA 23 and micro RNA 27 suppressed endothelial migration in response to vascular endothelial growth factor and cell proliferation. Zhou and his team found that micro RNA 23 and micro RNA 27 mediated angiogenesis through suppression of Sprouty 2, Semaphorin6A (Sema6A), and Semaphorin6D (Sema6D) (67, 68). Sprouty proteins inhibit insulin receptor and EGFR transduced MAPK signalling pathway (69). Sema6A is required for development of the thalamocortical projection and suppresses VEGFR2 signalling (70). Overexpression of micro RNA 23b or micro RNA 27b repressed Sprouty2, Sema6A, and Sema6D, whereas overexpression of both micro RNAs 23b and 27b showed an increased effect (68). Zhou et al.investigated the development of new blood vessels using a laser-induced choroidal neovascularization model (68). The expression levels of micro RNA 23a, micro RNA 27a and micro RNA 24 were significantly increased in the choroid region of the eye one week after laser injury. Intravenous injection of LNA-anti-micro RNAs targeting micro RNA 23 and micro RNA 27 resulted in knockdown of micro RNAs 23 and 27 in the eye instantly following laser injury. The choroidal neovascularisation area was repressed by more than 50 percent. Micro RNA 23 and micro RNA 27 represent interesting markers for therapeutic intervention in vascular diseases (67).
Inflammation is a protective reaction of vascularised connective tissue to injurious stimuli. Even though Inflammation is an important part of host defences, inflammation also results in ischemia and reperfusion damage. Micro RNAs are important in vascular inflammation especially activation of leukocyte and infiltration of...

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