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Osmosis In Plant Cells Essay

2099 words - 9 pages

Osmosis is the diffusion of water molecules from an area of high concentration of sucrose solution to an area of lower concentration of sucrose solution, through a selectively permeable membrane. The water molecules move down a concentration gradient in osmosis. Two diagrams of osmosis are shown in figures 1 and 2. Figure 1 shows the diffusion of water molecules through a selectively permeable membrane from an area of highly concentrated sucrose solution to an area of lower concentrated sucrose solution. Figure 2 shows a concentration gradient, in which the water molecules are diffusing down the concentration gradient.Depending on whether the higher concentration of sucrose molecules is on ...view middle of the document...

A diagram of such a cell can be seen in figure 4.3. A Flaccid CellA flaccid cell is what the state of the cell is like in between the two previously discussed stages. The cell wall in a flaccid cell is neither bulging nor bowed inwards.PredictionI predict that the higher the concentration of the sucrose solution the smaller the mass of the potato tissue will be at the end of the experiment. This is because the water molecules will diffuse out of the cell and it will then become plasmolysed, the higher the concentration of the solution the more water will diffuse out of the cell and the more plasmalysed it will become. A diagram of this can be seen in figure 4.VariablesIndependent Variable: There will be a range of five different bathing solutions - 2M, 1, 5M, 1M, 0.5M and 0M.Control Variable: I will control the length of the potato cylinders used, I will measure the length of the potato cylinders accurately using callipers, the mass of each cylinder will be measured accurately using a balance, I will time the experiment accurately, and I will control the excess solution on the cylinders when they are removed from the boiling tubes by rolling them on blotting paper.Dependent Variable: The mass of the tissue will be what is measured at the end of the experiment, and taken away from the measurements taken at the start of the experiment; I will then calculate change in mass for each duplicate in each concentration and then percentage change in mass for each duplicate. This will enable me to calculate the mean percentage change in mass for each concentration.Apparatus* 15 Boiling tubes* Corer (10mm)* Potatoes* Measuring cylinder* Beakers* Balance* Blotting paper* Labels* One sided blade* Cotton wool* Callipers* Salt solution* Distilled Water* The apparatus coloured orange will be used as basic equipment to set up the experiment.* The apparatus coloured in blue will be used for extra accuracy, the balance is accurate to 0.01g and the callipers are accurate to 0.1mm. These pieces of equipment will ensure that measurements are taken as accurately as possible.* The apparatus coloured in pink will be used to make the varied concentrations of salt solution.Safety PrecautionsIt is important to take care of safety when doing any experiment. In this particular one, it is necessary to make careful use of the corer as it could be dangerous due to the sharp edge, the blade should be taken care of for the same reason and the glassware could break and therefore could cut someone. Long hair should be tied back, everything should be moved from the work area, so that no-one might trip, blazers should be hung up, stools should be pushed in, and ties should be tucked in.Method1. Prepare five different concentrations of the sucrose solution using the table below, in order to make 40cm" of each solution. Prepare 3 of each.Concentration of solution (M) Volume of 2m Sucrose solution Volume of water2.0 30 01.5 22.5 7.51.0 15 150.5 7.5 22.50 0 302. Pour each solution into a...

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