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Osmosis Investigation Essay

1210 words - 5 pages

Osmosis InvestigationAimIn this investigation I aim to find concentration of a potato cell. I will do this buy putting slices of potato in to different concentrations of sugar seclusions so we may find the concentration that is isotonic with potato cell sap. A piece of potato in such a solution will neither gain nor lose any weight.HypothesisMy hypothesis is that the isotonic point will be between the 80 and 120g/l. I predict this because I have done a preliminary experiment that suggested this is where the isotonic point lay. I presume that the potato chip in the 50g/l will become turgid and gain in weight because the chip will have a lower concentration than the water and it will take in the water to make the water molecules the same inside as the outside of the chip. The potato chip in the 90g/l will become flaccid and lose weight because outside the chip there is a high concentration of sugar and the chip will give out water to make the water concentration outside equal to that on the inside of the chip.Scientific backgroundThis information is from is a special form of diffusion:the MOVEMENT OF WATERfrom a dilute solution to a more concentrated onethrough a PARTIALLY PERMEABLE MEMBRANE.This type of membrane (also called semi-permeable) allows only water, but not other (dissolved) substances to pass through.All cells are surrounded by a cell membrane, which is partially permeable, and the cytoplasm contains dissolved substances (solutes), e.g. sugars, salts, proteins. Whether or not water enters or leaves the cell depends on the relative concentrations of the cytoplasm and the fluid surrounding them.MethodI have conducted a previous experiment of this but it was not accurate, but I found that that the isotonic point was some where between 50g/l and 90g/l of sugar this is why I have decided to investigate between these amounts. Firstly I will label the glass beakers 'A' and 'B' so that each strength of solution is tested twice to make my results as accurate as possible. I then will fill both beakers with the same amount of 50g/l sucrose solution. Then I will slice the potato in to equal chunks, all pieces of potato must be then same size to make it a fair test. I must remove all skin from the potato, then rinse it in cold water to get rid of the damaged cells, I will then pat it dry with kitchen roll so that the water will not affect the weight. Then take initial weight. Then I must put the potato in the solutions but if we just lay the potato in there one of its sides will not be open to the solution making the experiment inaccurate. To over come this problem I have found a way to hang the potato off paper clips so that the whole surface area completely surrounded with the sucrose solution. Then I will leave me experiment for 30 minutes so that the osmosis may take place. Once that time is up I will take the potato out, dry it with kitchen roll and weight it on accurate weighing scales. I will then proceed with the...

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