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Oxidative Stress Aspect Of Rutin On Against 4 Nitroquinoline 1 Oxide Induced Experimental Oral Carcinogenesis

2110 words - 9 pages

Introduction:
Oral squamous cell carcinoma is the sixth most death causing common neoplasm worldwide with 600,000 new cases reported yearly (Dionne KR et al., 2014). Amid Asian countries India accounts for highest morbidity and 50-70% of mortality rate in all cancer (khandekar SP et al., 2006). In india the prominent site of oral cancer is the tongue (Krishna Rao SV et al., 2013). The major risk factor associated with OSCC in India is the habit of chewing betel nut leaves rolled with lime and tobacco a mixture called pan, which becomes a carcinogen due to longer exposure in the buccal mucosa of the oral cavity as well as alcohol, human papilloma virus, syphilis, oro-dental factors, dietary ...view middle of the document...

,2011) , is a flavanoid glycoside composed of quercetin aglycone bound to a rutinose ( rhamnose and glucose) disaccharide and serve as an antioxidant by donating electrons to neutralize ROS ,exerts metal chelating activities and protect against lipid peroxidation (Stanely Mainzen Prince P 2007). Tomato, orange, carrot, sweet potato, black tea and apple peels are the copious sources of rutin (Ihab T. Abdel-Raheem etal.,2010). Rutin has been showed possible protection against neoplasm, cardiovascular disease, liver diseases and various pharmacological activities including antibacterial, antiulcer, anti-inflammatory, antidiarrheal, antimutagenic, vasodilator and immunomodulatory activities (Janbaz KH et al., 2002).
Hence the present study was aimed to elucidate the chemopreventive role of rutin on 4 Nqo induced OSCC by manipulating lipid peroxidation (LPO), antioxidant tissue defense system, tumor marker enzymes. 607,258

Materials and method:
Chemicals:
4 Nitroquinoline-1-oxide and rutin were purchased from Sigma-Aldrich Co.Ltd., banglore, india. All other chemicals were of analytical grade procured from SRL Chemicals, Mumbai, India.
Animals:
Healthy wistar strain albino rats, weighing 120-150 g (8 weeks old) obtained from Veterinary College, Chennai, were used throughout the experiment. Animals were utilized according to the guidelines from the Institutional Animal Ethics Committee (IAEC No.06/03/2012). The animals were housed under conditions of controlled temperature (26 ± 2°C) with 12-hr day/night cycle. They were fed standard rat/mice pellet diet (Hindustan Lever Ltd., Mumbai) under the trade name Amrut rat/mice feed and were given access to water ad libitum.
Experimental design
The experimental animals were divided into four groups, each groups comprising of six animals.
Group 1 Control animals were treated with normal diet and drinking water for 20 weeks.
Group 2 Oral carcinoma was induced by administration of 50ppm 4NQO dissolved in drinking water for 20 weeks (4NQO alone) (Madankumar A et al., 2013).
Group 3 Animals were treated with Rutin (50 mg/Kg b.wt.) thrice a week orally. Rutin treatment was started one week prior to the first dose of 50 ppm 4NQO administration (as in group 2) and continued till end of the experimental period (4NQO + Rutin).
Group 4 Animals were treated with Rutin (50 mg/Kg b.wt) thrice a week orally for 20 weeks to assess the cytotoxicity if any, induced by Rutin, and rats were referred as drug control (Rutin alone).
At the end of the experimental period, the animals were fasted overnight and anesthetized with diethyl ether sacrificed by cervical decapitation. Tongue tissues were immediately excised and the tissue was tongue was homogenized in 0.1 M Tris-HCl buffer (pH 7.4). Homogenates was taken for the biochemical parameters.
Biochemical evaluation:
The total protein was estimated from the excised tongue by the method of (lowry et al., 1951)
I Assay Of Enzymatic Antioxidants
Antioxidants like Superoxide...

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