“Photosynthesis is the process” in that plants, a few bacteria, and a few protist use light energy to produce sugar and then converts that sugar using cellular respiration into ATP. Then convert the unworkable light energy from the sun into “usable chemical energy” to use with chlorophyll (Farabee, “Photosynthesis”). When it talks about how NADPH is being reduced, it just means that there is a continued discharged of “electrons from water to” it (Farabee, “Photosynthesis”). Then that “energy is used in carbon fixation and at that time no NADH is made only ATP (Farabee, “Photosynthesis”). Sometimes the cell lacks ATP so it makes more or if their is no more NADP + to be reduced into NADPH (Farabee, “Photosynthesis”). When water is split in photosystem I and II to gain electrons (“Electron Transport in photosynthesis.”). A Proton Gradient is established with the help of the Electron Transport that then powers the production of ATP (“Electron Transport in photosynthesis.”) . Then stores energy in the reduced form of the coenzyme of NADPH (“Electron Transport in photosynthesis.”). The energy then is used in the Calvin Cycle to make sugar and other carbohydrates (“Electron Transport in photosynthesis.”). Light is a very important factor in photosynthesis.
Light influences Photosynthesis in number of ways. If the light is very intense the plant grows better than if the light has a low intensity. When the chloroplast has no light present, the chlorophyll wouldn't be the color green that we see. Also if their was no light present then there wouldn't be photosynthesis to take place. When the enzymes in photosynthesis become denatured their is a “loss of tertiary structure”(Salvucci et al) Heat puts stress on photosynthesis by lowering the activation.(Salvucci et al) DPIP is important to photosynthesis when NADH is unavailable.
DPIP is a blue compound that is used as an electron acceptor (“Using DPIP as an Electron Acceptor”). DPIP is used to show if the light reactions are taking place (“Using DPIP as an Electron Acceptor”). It changes colors when light hits the chloroplast and the electrons get excited and reduces the DPIP from the chlorophyll (“Using DPIP as an Electron Acceptor”). The color of the DPIP changes from blue to colorless meaning that the DPIP accepts the electrons (“Using DPIP as an Electron Acceptor”). Since their was five test tubes that means there will be five hypothesis.
The hypothesis was that Test Tubes 1,2,4,5 would show no reaction. And Test Tube 3 would show a positive reaction. In Test Tube 1 there was no DPIP so that the electrons could be accepted by. In Test Tube 2 there was unboiled chloroplast and was put in the dark. Which photosynthesis wouldn't be able to take place because it needs light to...