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Population Growth Of Yeast And Effects Of Various Substrates On This Population Growth

1951 words - 8 pages

Population Growth Of Yeast And Effects Of Various Substrates On This
Population Growth

Plan: 1. Introduction;

Yeast is a unicellular fungus which reproduces asexually by budding or
division, as in the case of the genus Saccharomyces, which is
important in fermentation in food (walker, 1998). Yeasts are widely
distributed in nature.

Like bacteria and moulds, they can have beneficial and non-beneficial
properties in food production. The most well known examples of yeast
fermentation are in the production of alcoholic drinks and the
leavening of bread. Although there is a large range of species of
yeasts, only a few species are used in the food industry. These
species are usually either Ascomycetous yeasts or yeasts that belong
to the genus Candida. Unlike most Fungi yeast cells are round or oval
in shape.

2. Aim-

The aim of the investigation is to discover whether different
substrates of carbohydrates have a direct effect on the population
growth of yeast cells. This will be done by direct counting of cells
by haemocytometer to discover the average count of cells. This process
shall be over a time period of 5 days.

3. Method-

Apparatus required;

* For the yeast culture ;

* 6g of dried active bakers yeast ;

* 6 sugars (enough for 6 yeast cultures) in this investigation the
sugars used were:

* Glucose, Fructose, Dextrin, Ribose, Galactose and Sucrose;

* 600 ml of Distilled water;

* 6 Conical flasks with air blocks (in this instance cotton wool
with suffice);

* Compound microscope with lamp;

* Water bath set at constant 37ºC;

* Haemocytometer;

* 1cm³ pipette and filler;

* Boiling tubes and rack ;

* Supply of distilled water (last two needed for the serial dilution
of the culture to allow ease of counting -as explained in
preliminary investigation)

* Capillary tubes

* Timer capable of hours NOT minuets

* Thermometer

* Lens cleaner cloth

* Spatulas and top pan balance

* Filter paper OR beaker (as long as weight is taken into
consideration).

4. Reasons for the choice of apparatus-

· Yeast culture; Pre trials (shown later) indicate that a 1% yeast
culture diluted to 0.1% suspension allowed for ease of counting
(details of serial dilution shown later).

· Compound microscope; yeast cells are too small to be counted with
the naked eye. A microscope is the obvious choice.

· Water bath; Room temperature is too cold for the yeast cells to
multiply efficiently; a constant temperature of 37º shall be required.
This temperature will need to be checked on a regular basis (every 12
hours in this instance).The temperature dial on the water bath will
prove advantageous to control the temperature throughout the
experiment.

·...

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