Aseptic technique is a combination of principles and practices used during experiments to prevent or reduce the possibility of unwanted microorganisms from getting into Cell cultures, sterile solutions and supplies. This is more serious when working with human cell lines. Good aseptic technique is very important and basic for successful results in cell and tissue cultures or any other experiment. When a very good aseptic technique is followed, these principles and practices provides many benefits for your cultures such as Protecting the cell line from microbial and cellular cross contamination, Preventing compromise of the cell line by misidentification, Protecting the value of your cell line, experiments and cell culture processes. So here are some aseptic techniques;
PERSONAL HYGIENE AND PROTECTION:
→ Wear clean lab coats; for additional protection in the hood use a fresh, closed front lab coat with gloves that overlap the cuffs. Protective eyewear should be used when appropriate. Lab coats used for cell culture should not leave the cell culture area.
→ wearing clean gloves during aseptic procedures because if we have dry skin and if this skin somehow gets in to your experiment, your result will be affected. This is because flakes of dry skin are loaded with bacteria. The gloves can also protect you.
→ take care when handling sharp equipment this is to avoid the risk of broken or splintered glass.
→ Hair should be tied back or covered up because hair draws dirt/dust and it can cause contamination.
→ clean your table or culturing area before starting; this is because microorganisms can easily attach to dust particles, so cleaning the table will help us reducing the amount of dust and dirt in the culture area. Also do not open windows or use window fans that allow in outside air during your experiment If possible, this also to reduce the amount of dirt carried inside.
→ avoid water baths if possible because wetting the outside of a bottle with contaminated water before bringing it into a use is never a good idea.
→ Flame the neck of the bottles you are using before and after pouring because this will prevent air bulb contamination getting into the tube.
→ Always use separate media bottles to reduce both potential contaminations; limits the spread of contamination and cross contamination with another cell line if the bottle of medium becomes contaminated.
→ Avoid sharing bottles of media or other solutions with class mates because this can cause cross contamination and also lack of responsibility is started from sharing with others.
→do not use same pipette for different cell line; this is to prevent the chance of easy widespread contamination by other cell lines or mycoplasma.
→ Avoid spills or liquid bridges on the lips of dishes, bottles and flasks this is because it provide an easy entry point for microorganisms into the vessel. But if it happen replace the dishes, caps or flasks that have wet...