Proteomics Techniques Overview Essay

3278 words - 13 pages

AbstractProteomic techniques reviewed in this paper looks into how proteomics grew and came into existence, from basic protein chemistry to high through put technologies. We also look into draw backs that come with it and also look into their possible solution. And how advances in methods and technologies have expanded our understanding of biological systems better. Finally we talk about how each technique can be used in relevance to the experiment we are trying to carry out.Proteome constitute the complete profile of proteins expressed in a given tissue, cell or an organism at a given point of time. Proteomics – the science and proteomic techniques was the direct result of mapping and sequencing projects. The goal then was the rapid identification of all the proteins that a cell or tissue could express[1, 2] using all the techniques possible under the sun. The most common problem faced with the above was the dynamic range that the quantification methods needed to cover[3]. Proteins vary in concentrations and the more realistic goal was the determination of diverse properties of proteins, identification and quantification.Proteomic techniques for the rapid identification and quantification of proteins have been and are still being developed as we speak. There no single hard and fast rule as of date, no such mature technology in proteomic techniques exists.In this review we shall come across proteomic techniques and its current status while comparing the similarities in techniques of the past to now. And also explore the advancements in the techniques and there by distinguish the new from the old fashioned, ways of get things doneResearch way back in the 80’s on proteins was based on crude understanding of protein chemistry. The approach was observation of the phenotype or function to the genes responsible for the function and latter on the identification of products that manifested the observed phenotype. This approach came to be known as ‘forward’ approach. With the advent of large scale sequencing projects the approach changed and it was now ‘reverse approach’. It looked at gene sequencing to function and phenotype. These approaches used techniques such as expression arrays or serial analysis of gene expression (SAGE)[4, 5]. The above techniques pioneered the development of more global approaches to the quantitative analysis of proteins expression by high resolution two dimensional gel electrophoresis or ‘2DE’[6, 7].Studies in the early 90’s on DNA sequencing gave us the complete genome sequence libraries of many model organisms such as yeast[8] and latter on human[9, 10]. With these complete libraries in hand; rapid identification was matter of extracting the sequence information from proteins and peptides followed by correlating it to the databases. This paved the entry of mass spectrometric techniques and the use of database search algorithms.Mass spectrometers were the tool house of analytical...

Find Another Essay On Proteomics Techniques Overview

Enlightenment Thought in New Zealand Schools

1594 words - 6 pages In this essay I will be looking at how the political and intellectual ideas of the enlightenment have shaped New Zealand Education. I will also be discussing the perennial tension of local control versus central control of education, and how this has been affected by the political and intellectual ideas of the enlightenment. The enlightenment was an intellectual movement, which beginnings of were marked by the Glorious Revolution in Britain

Psychological Egoism Theory Essay

2240 words - 9 pages The theory of psychological egoism is indeed plausible. The meaning of plausible in the context of this paper refers to the validity or the conceivability of the theory in question, to explain the nature and motivation of human behavior (Hinman, 2007). Human actions are motivated by the satisfaction obtained after completing a task that they are involved in. For example, Mother Teresa was satisfied by her benevolent actions and

How Celtic Folkore has Influenced My Family

1587 words - 6 pages Every family has a unique background that influences the way they live and interact with other people. My parents, who emigrated from Ireland to the States with my three brothers in 1989, brought over their own Celtic folklore and traditions that have helped shaped the way our family operates and lives. One aspect of folklore that has helped shape my family dynamic is the Celtic cross—both its background and what role it has played in our lives

Julia Margaret Cameron

1406 words - 6 pages At a time when women were looked upon as being homemakers, wives, mothers and such the late 1850's presented a change in pace for one woman in specific. Photography was discovered in 1826 and soon after the phenomenon of photography was being experimented with and in turn brought new and different ways of photo taking not only as documenting real time, but also conceptualizing a scene in which an image would be taken. Julia Margaret Cameron will

Evaluation of School Improvement

1403 words - 6 pages The evaluation process should be progressive to incorporate overall planning, implement changes, which contribute to success. In order to focus on school climate and norms, the evaluation design must include the students, instructions, and outcomes to improve communication and building-level concerns to be address in this response. School Climate and Social Norms The school principal, other staff leaders, and personnel set the tone and the

Case Study: The Benefits of Animal Testing

1757 words - 7 pages Nine year old Amy has already had a rough start in life. She was born with an abnormal heart that hinders her everyday activities. Amy is unable to keep up with kids her own age because she often tires out easily. As a consequence, she has very little friends and is often alone. Amy is forced to take different medications everyday just to survive. Amy’s life consists of medicine, doctors, and constant hospital visits. However, Amy is due for a

Myth and Magic: Realism in "One Hundred Years of Solitude"

1531 words - 6 pages “He enjoyed his grandmother's unique way of telling stories. No matter how fantastic or improbable her statements, she always delivered them as if they were the irrefutable truth” (Wikipedia, 2011). Experiences are particular instances of one personally encountering or undergoing something and in these moments of time life changes for the best or the worst and memories are formed. These recollections such as riding your first bicycle, going to

Adiponectin: a Novel Indicator of Malnutrition and Inflammation in Hemodialysis Patients

2384 words - 10 pages IL-6 and Biomerica Intact for PTH). Blood urea nitrogen (BUN), serum albumin, creatinine, transferrin and lipid profile were measured with standard automated techniques. Nutritional Status Assessment The nutritional status (any changes in weight , appetite, gastrointestinal symptoms) was evaluated through subjective global assessment (SGA) questionnaire (10) that was asked in person directly by principal investigator. According to the

The Congo Free State: A Legacy of Apathy, Exploitation and Brutality

2298 words - 9 pages Between 1885 and 1908, Belgium’s Leopold II ruled Congo, a region in central Africa, as his personal colony, exploiting the resources and inhabitants for his own gain. Leopold allowed and encouraged Europeans and other Westerners to enter Congo and set up companies whose primary purpose was to gather rubber, which was abundant but difficult to get to in the Congo, using the Congolese as the laborers for the Europeans. Rubber gathering in Congo

Selective Exposition in The Lottery, by Shirley Jackson

1073 words - 4 pages Usually when someone hears the word “lottery” the first thing that comes to mind is a large sum of cash that people compete against highly impractical odds to win. Shirley Jackson’s story The Lottery might imply a similar conception based on the title alone, but the story is filled with unknowns never revealing exactly when and where the story takes place, or why the lottery exists; even what the lottery is isn’t revealed until the very end. Yet

Lightning

1857 words - 7 pages INTRODUCTION I remember when I was a young child; I would always be scared whenever there was a severe storm outside that included thunder and lightning. This was especially true in the hours of darkness, when you could really see the lightning. As I grew older this so-called fear of lightning turned into a fascination for this weather phenomena. One of my most vivid memories of lightning as a young man was when I was flying to Florida, the

Similar Essays

Study Of Bioinformatics, Proteomics, And Genomics

2125 words - 9 pages development (Chris S., 2003). 2.1 Applications of Bioinformatics in Biotechnology 2.1.1 Bioinformatics in Proteomics Proteomics is one of the branches of biotechnology which deals with the methods of molecular biology, biochemistry, and genetics to analyse the morphology, its role and interactions of the proteins which are generated by the genes of a specific cell, tissue, or organism. This particular is being developed constantly and novel techniques

When The Bubble Burst Essay

1539 words - 6 pages By the time I arrived state side from my second tour in the Middle East the housing bubble had already burst. I noticed a drastic change in the way that many of my friends and family were living. Several of my friends that worked in real estate had sold their boats and seconds houses. My own stock portfolio had lost a third of its value. My sister and her husband had defaulted on their home mortgage leaving them scrambling for a place to live. I

Phase Diagram Essay

4456 words - 18 pages Introduction: Chemical equilibrium is a crucial topic in Chemistry. To represent and model equilibrium, the thermodynamic concept of Free energy is usually used. For a multi-component system the Gibbs free energy is a function of Pressure, Temperature and quantity (mass, moles) of each component. If one of these parameters is changed, a state change to a more energetically favorable state will occur. This state has the lowest free energy

Revolutionary Work Of Art Essay

1890 words - 8 pages Walter Benjamin emphasizes in his essay, “The Work of Art in the Age of its Technological Reproducibility” that technology used to make an artwork has changed the way it was received, and its “aura”. Aura represents the originality and authenticity of a work of art that has not been reproduced. The Sistine Chapel in the Vatican is an example of a work that has been and truly a beacon of art. It has brought a benefit and enlightenment to the art