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Rate Of Reaction Using Enzymes Essay

3788 words - 15 pages

Rate of Reaction Using Enzymes

In this bit of coursework I will be investigating the rate of reaction
in which enzymes are the catalyst breaking down a substrate. The
substrate I am going to use is Hydrogen Peroxide (H2O2, water with
another Oxygen), this molecule usually breaks down on its own accord,
but also the rate of decomposition can be increased with the use of a
catalyst; in this case the catalyst is a biological one called an
enzyme. Hydrogen Peroxide slowly breaks down on its own accord into
water (H2O) and oxygen (O2):

2 H2O2 à 2H2O + O2

Hydrogen Peroxide à Water + Oxygen

In my experiment, I want to investigate the rate of reaction with an
enzyme, and the enzyme I will add to the Hydrogen Peroxide will be
Catalase. The enzyme will accelerate the rate of reaction without
being used up, for that is a property of a catalyst is, it speeds up a
reaction without being used up, and an enzyme is a biological
catalyst.

[IMAGE]

Method

Before doing my 'actual' experiment from which I would take my data
and ultimately come up with a conclusion I did a preliminary
experiment, which was for a number of reasons. Firstly, I had no
previous experience of doing this reaction and so needed an idea of
how it went: how quickly, how much oxygen was produced and what
measurements to use. Also there were two different experiments in
which I could have got my data from, one of the experiments was
relatively simple and the other more complex but also more accurate.

The first experiment I had a choice to do was one in which I had a
rack of six test tubes; the procedure I had to follow was simple, I
had to put 5cm3 of different concentrations of Hydrogen Peroxide
ranging from 0%-100% in each of the test tubes slowly, this was so I
did not lose oxygen by giving the substrate energy to break down
before the experiment had started. Then I added three drops of
detergent to the substrate, this was so when it broke down and
released oxygen the bubbles would be trapped in the detergent and as
more oxygen was released then the level of bubbles would increase; I
did this for all six of the test tubes using the 0% (100%
water)concentration as my control.

I did not do the other experiment that was with the gas syringe that
measures accurately the amount of oxygen given off; I did not do this
experiment because I had already concluded that I would do it to get
my results, because the other type of experiment was too inaccurate
for my investigation. The other experiment measured roughly how much
oxygen was given off but the gas syringe experiment measured
accurately the amount t of oxygen given off and so would be more
useful for my investigation.

Equipment

The equipment I will need so I can do the gas syringe experiment is:

* Dry gas syringe-if wet it can...

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