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Investigation Of Action Of Saliva And Hydrochloric Acid In Two Carbohydrate Solutions

1647 words - 7 pages

Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions Objective : To investigate the action of saliva and 3 hydrochloric acid in two carbohydrate solution Results Table1: Observation Conclusion Solution A Benedict's test: Blue coloration turned to brick red precipitate. Reducing sugar is present in the solution A. Iodine test: The coloration remained unchanged. Starch is absent in solution A. Solution B Benedict's test: The blue coloration remained unchanged. Reducing sugar is absent in solution B. Iodine test: The coloration turn into dark blue coloration. Starch is present in solution B. Table 2: Tube Contents Temperature(°C) Benedict's Test-Colour Observation After min 5th min (from tubes 1 - 4 into 1' - 4') After 35th min (from tubes 1 - 4 into 1' - 4') 1 10 ml solution B 1 ml saliva 37 Blue coloration turned into translucent green. Blue coloration turned to green and finally moderate amount of brick red precipitate suspended in solution. The solution was opaque. 2 10 ml solution B 3 M HCI 37 The blue coloured solution remains unchanged. The blue coloured solution remains unchanged. 3 10 ml solution B 3 M HCI 95 White coloured translucent suspension formed in moderate amount. A very big amount of white suspension formed which is very opaque. Turned back into translucent and remaining solution was blue which is translucent. 4 10 ml solution B 1 ml saliva 95 The blue coloured solution remains unchanged. The blue coloured solution remains unchanged as well. Discussion : Enzymes are biological catalysts that carry out thousands of chemical reactions that occur in living cells. Most of them are proteins. They speed up metabolic reactions in the body but remain chemically unchanged themselves In the experiment, the enzyme amylase was involved.this enzyme takes part in the digestion ofcarbohydrate. digestion is the break down of large complex and insoluble food into small simple soluble molecules for the body to absorb it. starch is a complex, the body have to find a way to break down starch, so the body produces salivary amylase in the mouth to begin the break down of starch into maltose. As the temperature rises, reacting molecules have more and more kinetic energy. This increases the chances of a successful collision and so the rate increases. There is a certain temperature at which an enzyme's catalytic activity is at its greatest . This optimal temperature is usually around human body temperature (37.5 oC) for the enzymes in human cells. Above this temperature the enzyme structure begins to break down (denature) since at higher temperatures intra- and intermolecular bonds are broken as the enzyme molecules gain even more kinetic energy.Also Enzymes have an active site. This is part of the molecule that has just the right shape and functional groups to bind to one of the reacting molecules. The reacting...

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