Effects Of Solutes With Varying Tonicities On Sheep Red Blood Cells

862 words - 3 pages

Effects of Solutes with Varying Tonicities on Sheep Red Blood CellsIntroductionThe objective of this lab is to evaluate the effects of solutions with varying tonicities on the sheep red blood cell (SRBC) by examining the response of the cell to varying osmotic conditions (specifically the effect of hypertonic, hypotonic and isotonic solutes on cell membranes.) Henri Dutrochet, (Nov. 1776 - Feb. 1847), a French physiologist, discovered and named the phenomenon of osmosis. [1] Osmosis is the movement of solvent molecules through a selectively permeable membrane into a region of higher solute concentration, with the goal of equalizing the solute concentrations on both sides of the cell. The difference between osmosis and diffusion is that diffusion is the process where molecules move from an area of high concentration to an area of low concentration while in osmosis the molecules move across a selectively permeable membrane. Diffusion and Osmosis are both types of passive transport. This is because no energy is needed for the molecules to move in or out of the cells. Because biological membranes are semipermeable to larger and/or polar molecules including, but not limited to, proteins, polysaccharides, ions and water, osmosis is needed to facilitate many chemical reactions throughout the body (cells).Osmotic pressure is the force that moves water through the membrane. A Hypertonic solution has more solute which means there is less water than in the cell. A cell placed in this solution lose water causing the cell to become crenated. A Hypotonic solution has less solute (so MORE water) than the cell. A cell placed in this solution will take up water (osmosis) and blow up. An Isotonic solution has just the right amount of solute for the cell. A cell placed in this solution will stay the same. [2] How will the SBRC respond when placed in isotonic, hypertonic and hypotonic solutions? To test this hypothesis, the SRBC will be placed in 0.9% NaCl, 10% NaCl and distilled water.HypothesisIf crenation is related to high salt concentration then placing a cell in a hypertonic solution will result in crenation of the cell.If hemolysis is related to low salt concentration then placing a cell in a hypotonic solution will result is a hemolytic reaction in the cell.If equal movement of water and sodium in and out of the cell is related to an equal salt concentration then a cell placed in an isotonic solution will result in no change in the cell.If a cell is not exposed to any change then there should be no change within the cell.MaterialsGlovesGoggles.Sheep bloodMicroscopes, microscope slides, cover slipsEye dropperIsotonic solution (0.9% NaCl)Hypotonic solution (Distilled water)Hypertonic solution (10% NaCl)Biohazard containersDisinfectantPaper towelsProcedure1. Wash hands and don...

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