Background: Breast cancer is the second leading cause of death among women worldwide. Apart from the available procedures of diagnosis for breast cancer identification of an ideal biomarker may help in reducing the incidence of breast cancer. The study was aimed to estimate and compare the sialic acid levels in serum of breast cancer patients and healthy individuals to elucidate the importance of it as a potential biomarker in breast cancer.
Materials and Methods: The study was carried out from February 2012 to September 2012 in cancer block St.Anns general and cancer hospital, Kazipet, Warangal, India. A total of 80 members were enrolled in the study out of which 55 were breast cancer patients and 25 were healthy individuals from whom the required data and informed consent was obtained, and then serum was analyzed using diphenylamine method and spectrophotometry.
Results: Serum sialic acid levels of healthy subjects were compared with that of the breast cancer patients and a large difference was found which represents very higher serum sialic acid levels of breast cancer patients and very lower levels in healthy subjects in accordance with the normal range i.e., 15.4 mg/dl. Out of 55 breast cancer patients established breast cancer patients [32(60%)] have shown larger serum sialic acid values than newly diagnosed breast cancer patients [23(40%)]. Statistical analysis was performed using SPSS software and the P-value was found to be <0.0001 which is significant.
Conclusion: At present, as there is no single treatment known to bring a definite cure for breast cancer, one of the possible solutions for combating breast cancer is through identification of reliable biomarkers that can be effectively used for early detection, prognosis and treatments of the cancer. Serum sialic acid is one such protein which can be used as the biomarker in breast cancer. Apart from this awareness about breast cancer and risk factors could help in prevention and reduction in number of cases.
Key words: Sialic acid, Breast cancer, diphenylamine method, spectrophotometry, SPSS software.
Breast cancer is defined as the malignant tumour that initiate in the cells of the breast characterized by a group of cancer cells that can grow into (invade) surrounding tissues or spread (metastasize) to distant areas of the body and upon progression may result in metastatic breast cancer. Breast cancer is the second leading cause of death among women worldwide (Alteri R et al., 2012). According to the American cancer society, more than 2, 30,000 women will be diagnosed with breast cancer annually in the USA, and more than 39,000 will die from the disease. In India, the number...