Soil And Seed Sterilization Effects On Dwarf Pea Plants

1515 words - 6 pages

The growth of dwarf peas, Pisum sativum depends on the characteristics of the soil and the seeds. Sterile conditions will have a positive effect on P. sativum below and above ground physiology. The plants’ root length, root width and number of leaves will be positively affected by sterile conditions because the pathogens in soil which out compete the plants for its nutrients will be killed off. Soil and some seeds were sterilized and grown for twenty one days before root length, root width, and number of leaf parameters was tested. From the three parameters, root length was the only one affected by sterile conditions. No changes were observed in the other parameters because more growth time for P. sativum plants was required.


Intro
Soil is one of the greatest contributors to plant growth because it consists of nutrients such as potassium, nitrogen, phosphorus and living organism. In the vast plant world, some living organisms in soil and plants such as rhizobia, which are the bacteria that supply sufficient nodulation in legume crops” (Mathew, David, & Mark, 2013) are useful. It’s these important rhizobia bacteria located in the plants’ root nodules that aid in nitrogen fixation for the plant. Other living organisms such as nematodes and parasites are harmful to the plants’ health. The harmful living organisms in soil rob plants for their resources and nutrients leading to nitrogen and sulfur nutrient deficiencies. In highly valued nutritious food plants such as dwarf peas, Pisum sativum that contain an abundant content of essential elements such as starch; the presence of pathogens in soil will lead to low nutrition value content in the dwarf peas.
To overcome the challenges in soil and seeds that negatively affect plant growth and survival, new cultivation practices such as seed and soil sterilization are used. During sterilization, seeds and soil are exposed to steam from an autoclave at high temperatures for a certain period of time. At these high temperatures, most microbes in both soil and seeds are destroyed and without these microbes, plant growth is improved. In plant cultivation, exposing P.sativum seeds to sterile conditions will have a positive effect on the plants’ below and above ground physiology. If P. sativum seeds are exposed to sterile conditions, then there will be an increase in the plants’ root length, root width and number of leaves. The prior hypothesis and predictions were tested by carrying out an experiment.
Methods and Material
Using an autoclave, some seeds and soil were sterilized by exposing them to steam at 121 degrees ferinheight for 15-20 minutes whereas some soil and seeds weren’t. Both sterilized seeds and unsterilized seeds were planted in four pots in each group labeled A- F for a total of 6 groups. The planting arrangement was five sterilized seeds in sterilized soil, five sterilized seeds in unsterilized soil, five unsterilized seeds in sterilized soil, and five unsterilized seeds in...

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