This website uses cookies to ensure you have the best experience. Learn more

Summary Of: Apoptosis In Human Hepatoma Hep G2 Cells Induced By Corn Peptides And Its Anti Tumor Efficacy In H22 Tumor Bearing Mice

1532 words - 7 pages

Jiang-Tao Li, Jiu-Liang Zhang, Hui He, Zhi-Li Ma, Zhi-Kui Nie, Zhen-Zhen Wang, and Xiao-Gen Xu are affiliated with the College of Food Science and Technology, Huazhong Agricultural University & Key Laboratory of Environment Correlative Dietology, and the Ministry of Education, all in China. Their study of corn peptide effects on liver tumors in mice was funded by a grant from China’s National Natural Science Foundation and National “863” Program. They undertook this study to examine the effects of plant-derived chemicals on cancer. Cancers can be a result of any combination of dietary insufficiency, environmental factors, and/or genetic components. However, up to 95% of cancers are thought to be the result of diet and other lifestyle factors rather than the result of genetic factors (Bhanot et al 2011). The authors recognize this fact and that there is a proliferation of naturally based products to boost immune function and combat tumor growth and development. Corn proteins (CPs) are widely and readily available as they are by-products of the expansive corn products market. Because previous work with CPs had shown positive physical affects (such as lowering blood pressure and mitigating the harmful effects of alcohol on the liver), the authors chose to examine how CPs might modulate tumors and the immune systems of mice.
The corn proteins were prepared by separating them out of corn gluten meal using an ultra-filtration method. The CPs were lyophilized (sterilized powder) for use in the mouse experiment. BALB/c mice were used to grow H22, a type of liver tumor analogous to the human liver tumor HepG2. The BALB/c mice are a line of inbred mice that are not naturally prone to tumors (Festing 1998). The H22 cells were injected into the study mice through the right armpit of each mouse. The H22 tumor cells were allowed to grow for twenty-four hours. After the incubation period, the fifty mice were split into five groups of ten, at random. Each group was assigned a treatment method. They were injected in the abdomen each day for 10 days. The control group received a saline solution. One group received 20 mg/kg of CTX (cyclophosphamide, a chemotherapy agent). The remaining three groups received different concentrations of the CPs. The concentrations were 100 mg/kg, 200 mg/kg, and 400 mg/kg. The volumes of all the doses were 0.1 ml/10g. The tumors were measured on even days during the ten days of treatment. On the eleventh day, the all the subjects were weighed and then euthanized. Blood samples were collected to measure levels of interleukin-2, interferon-gamma, and tumor necrotizing factor-alpha. The tumors were removed and weighed. The spleen and thymus of each mouse were also removed and weighed. A second experiment was set up in the same way, but this time, the authors tracked how long the mice in each treatment group lived.
The results were based on the findings of the mouse tumors but also using in vitro HepG2 cell samples. The HepG2 cells...

Find Another Essay On Summary of: Apoptosis in Human Hepatoma HepG2 Cells Induced by Corn Peptides and Its Anti-tumor Efficacy in H22 Tumor Bearing Mice

Physical and functional interaction of the proto-oncogene EVI1 and tumor suppressor gene HIC1 deregulates Bcl-xL mediated block in apoptosis

650 words - 3 pages /co-transfection of HA-EVI1, flag-HIC1 and HA-EVI1 + flag-HIC1 in 293T cells, proteins were immunoprecipitated with anti-flag antibody and were resolved by SDS-PAGE electrophoresis. Western blot analysis showed that EVI1 was efficiently immunoprecipitated with HIC1 (fig.1, 1st panel, lane 3). The amount of immunoprecipitated HIC1 was shown in panel 2. The above result was further confirmed by reverse co-immunoprecipitation i.e. immunoprecipitating

Gene Therapy to Kill Tumor Cells Effectively

928 words - 4 pages middle of mda-7 backbone by overlapping PCR mutation, then its therapeutic efficacy evaluated in HepG2 cell line. They demonstrated that expression pattern and apoptosis induction of new RGD-IL-24 was similar to IL-24 expression plasmid (20). Theoretically this kind of design may overwhelm true protein folding at the end or disrupt some function although Pei et al didn’t mention it. Other few related experiments employed RGD targeting strategies for

Human Embryonic Stem cells and Induced Pluripotent Cells

1136 words - 5 pages , and different types of cells are being tested for their likelihood of being converted back into stem cells. Because of the controversy that using human embryonic stem cells has caused over the definition of life itself and how it is being used or abused by research scientists, it is easy to see how people can get swept away in looking toward something like induced pluripotent cells to replace embryonic research completely within the stem cell realm. Works Cited "Frequently Asked Questions." What Are Embryonic Stem Cells? [Stem Cell Information]. N.p., n.d. Web. 08 Mar. 2014.

Application of Mathematics in Modelling Tumor Growth

3621 words - 14 pages is hard to find data of tumor volume from a patient. Therefore I use in vivo tumor growth data collected from an experiment. In this experiment, a type of mouse is used for modeling a possible growth model for human tumors. Human KARPAS-299 cells were implanted into a SHrNTM mouse which is developed by Harlan laboratories. The mice were maintained in a barrier under controlled environmental conditions. Data of volume of tumor is collected and it

Application of Mathematics in Modeling Tumor Growth

3621 words - 14 pages is hard to find data of tumor volume from a patient. Therefore I use in vivo tumor growth data collected from an experiment. In this experiment, a type of mouse is used for modeling a possible growth model for human tumors. Human KARPAS-299 cells were implanted into a SHrNTM mouse which is developed by Harlan laboratories. The mice were maintained in a barrier under controlled environmental conditions. Data of volume of tumor is collected and it

Lengthy plot summary of "Chuck and Buck"; including a detailed explanation of its application to Human Sexuality functioning

1037 words - 4 pages immediately tells Buck to back off. Chuck then proceeds downstairs to Carlyn and the two leave together for Los Angeles. Buck's obsession with his old friend begins here as he packs up everything he owns and moves to a motel room in Los Angeles in hopes to rekindle the relationship he once had with Chuck. Buck insists on stopping by unannounced at Chuck and Carlyn's house and also at Chuck's office. In a short amount of time, Buck is introduced to

Oncogenes and Tumor Suppressor Genes Play a Role in Cancer

930 words - 4 pages . The risk of genetic testing could also result in facing discrimination with getting insurance coverage for employment, and the possibility for psychosocial or financial harm (McNatt 2013). I would recommend BRCA genetic testing to female family members because breast cancer does not occur only by gene inheritance. As I mentioned before, mutations can happen during a person’s life due to radiation exposure or chemical-induced mutations. Cells in

Elucidating the role of adhesion factors in circulating tumor cell clustering for breast cancer metastasis and venous thromboembolism

667 words - 3 pages microtubule nucleation), a process shown previously only in plant and yeast cells, in human breast cancer cell lines. We aim to determine if the incidence of cytoplasmic microtubule nucleation increases in cell lines with greater metastatic potential. 2) Do metastatic breast tumor lines have an imbalance of soluble versus insoluble microtubule nucleating proteins? In normal interphase cells, approximately 80% of γ-tubulin is thought to be in a

Effects of Gene p53 the Tumor Repressor

917 words - 4 pages presented by MHC and becomes stimulated. The stimulated T cells then divide and look for other cells bearing the same antigenic peptide. RESULTS Only the mice immunized with the mutant p53 peptide fused to the E3 leader sequence are protected. Induction of cytotoxic T lymphocytes (CTLs) is most effective in the tumor-bearing mice injected with the mutant p53 fused to the E3 leader sequence. The CD8+ phenotype of T lymphocytes is necessary

Useful Technogies Used by Cardiac Tumor Surgeons

1133 words - 5 pages discusses successful tests with targeting cells, leaving healthy cells; the previous literature suggests it’s in development. Figure 1, image of a nanobot. Figure 1 (How Stuff Works, 2007). Camera is used to track the device; it releases the payload at the correct location. 3. Da vinci System This robot does surgical operations. This site is useful as it explains that it works by the surgeon directing the robot and controlling the motions

Summary Of Chapters And Themes In Of Mice And Men

2740 words - 11 pages answers every time for Lennie which makes the boss curious. He asks George what his interest in Lennie is, thinking that George is out to get Lennie's pay. George told the boss that Lennie had been kicked in the head by a horse when he was little, and he had told Lennie's aunt that he would take care of Lennie. When the boss left, Candy came back in with his dog after listening to the conversation through the door. Curley, the boss' son, came in

Similar Essays

Tumor Protein 53 And Its Role In Ovarian Cancer

3174 words - 13 pages TP53, also called tumor protein 53 is a tumor suppressor gene that encodes p53 and acts as a control center for the cell to act on when stressed (Brachova). Human p53 is a nuclear phsophoprotein of molecular weight 53kDa located on chromosome 17 containing 11 exons and 10 introns (Ling). One of its primary roles is as a transcription factor and in its active state is a homotetramer comprised of four 393 amino acid residues (Joerger , The tumor

Genes' Response Of Tumor Cells To Apigernin

1459 words - 6 pages (synonym: WAVE1) and regulates P-glycoprotein expression in leukemia cells [37]. This gene is overexpressed in leukemia and inhibits apoptosis. Transfection with WAVE1 rendered tumor cells resistant to anticancer drugs, while RNA interference restored chemosensitivity [37]. Furthermore, WAVE1 regulated the anti-apoptotic Bcl-2 protein and WAVE1 depletion decreased production of reactive oxygen species by anticancer drugs[38]. The zinc finger protein 124

Physical And Functional Interaction Of The Proto Oncogene Evi1 And Tumor Suppressor Gene Hic1 Deregulates Bcl X L Mediated Block In Apoptosis

731 words - 3 pages to 120 amino acids (aa); HIC1-121 to 438 aa; HIC1-439 to 612 aa and HIC1-613 to 714 aa were cloned into flag-pCMV vector (Sigma, USA). Similarly, EVI1-∆ (24-239 aa) (first set of zinc finger) was generated by cloning the EcoRI and SalI digested PCR amplified EVI1 fragment in HA-pCMV vector (Clontech, Japan) (Pradhan, Mohapatra, 2011b). 2.2 Cell culture and transfection Human colorectal carcinoma cell line, HCT116 (p53-/-) and embryonic kidney

Physical And Functional Interaction Of The Proto Oncogene Evi1 And Tumor Suppressor Gene Hic1 Deregulates Bcl X L Mediated Block In Apoptosis

662 words - 3 pages EVI1 and HIC1 form a complex in cells we studied their colocalization in 293T cells. Transiently transfected 293T cells with HA-EVI1 and flag-HIC1 were stained with anti-flag and anti-HA antibodies followed by secondary antibodies tagged with Alexa 488 and Alexa 594 respectively. We have reported earlier that in 5-10% of the cells EVI1 is present in nuclear speckles (Chakraborty, Senyuk, 2001). HIC1 protein is known to oligomerize via the N-terminal