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Testing For Macromolecules Essay

618 words - 2 pages

Purpose:To determine which chemical indicators that will indicate the presence of glucose, starch, lipid, and protein in various samples.Methods and Materials:For materials, please refer to p.18 in Biology 11 (replace Sudan IV with filter paper)For procedures, please refer to p.18-19 (except Part C)For Part C in Inv. 1A, please read the following procedures:1.Label sample 1-6 on the filter paper2.Add one drop of each sample corresponding to their number indicated on the filter paper (for the number of each sample, please refer to step 4 in Part A on p.18)3.Leave the filter paper to dry for approximately 5-10 minutes4.Record observationsResults:Table 1: Reactions of sample substances to indicatorsSAMPLEBenedict's solution + heatIodine solutionFilter paperBiuret reagent1.protein solutionPurpleNo changeDried upPurple2.vegetable oilNo changeNo changePaper remained translucentNo change3.glucose solutionOrangeNo changeDried upNo change4.sucrose solutionNo changeNo changeDried upNo change5.starch solutionNo changeDark blue-blackDried upNo change6.distilled waterNo change (Clear blue)No change (Clear yellow)Dried upNo changeInvestigation 1A: Testing for MacromoleculesDiscussion:By observing the table above, we could see that Benedict's test was for reducing sugars, iodine test was for the presence of starch, filter paper was for the presence of fatty acids, and the Biuret test was for amino groups present in proteins. Benedict's solution was used to test for the presence of simple sugars, such as glucose (monosaccharide). When heated, the solution mixed with monosaccharides produced a reddish-orange colour. This was because Benedict's solution is composed of sodium citrate, sodium carbonate, and cupric sulfate pentahydrate. When solution is heated, an oxidation-reduction reaction occurs: cupric ion (Cu+2) oxidizes into a cuprous ion (C+) and precipitates into cuprous oxide (Cu2O) because Benedict's solution loses an oxygen (Cu+2 ---> Cu+ forming Cu2O). Basically, it is a reaction between the aldehyde...

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