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The Effect Of Osmosis In Potato Cells With Different Concentrations Of Sucrose Solution

2249 words - 9 pages

The effect of osmosis in potato cells with different concentrations of sucrose solution

Aim: To test the effect of different concentrations of sucrose
solution to osmosis in a potato cells by putting potato pieces in test
tubes of water containing different concentrations of sucrose
solution.

Scientific Theory:

Osmosis is defined as the movement of water molecules across a
partially permeable membrane from a region of high water concentration
to a region of low water concentration. In a high concentration of
water the amount of solute (e.g. sugar) is low. This could be called a
weak or dilute solution. In a low concentration of water the amount of
solute (e.g. sucrose) is high. This could be called a strong or
concentrated solution. When two such solutions are divided by a
partially permeable membrane the water will move from the area of high
concentration to the area of low concentration, until both sides are
equal (have reached equilibrium).

Knowing that osmosis will occur across a partially permeable membrane
whenever there is a difference between the water concentrations on the
two sides of the membrane, and knowing that when this happens to cells
they will either become turgid if water flows into them, or flaccid if
water flows out of them, and thus change their volume, we want to test
the hypothesis that:

If the concentration of a solution into which a cylinder of potato is
placed is greater than a certain level the cylinder will contract, and
if the concentration is less than that level it will expand. The cell
membrane in cells is partially permeable and the vacuole contains a
sugar/salt solution. So when a cell is placed in distilled water (high
water concentration) water will move across the partially permeable
membrane into the cell (lower water concentration) by osmosis, making
the cell swell. This cell is now referred to as turgid. If done with
potato cells the cells would increase in volume and mass because of
the extra water. If these potato cells were placed in a solution with
a low water concentration, then the opposite would happen. Water would
move out of the cell into the solution. In extreme cases the cell
membrane breaks away from the cell wall and the cell is referred to as
flaccid. The potato cells will have decreased in volume and mass.

The greater the concentration of water in the solution the greater the
amount of water that enters the cell by osmosis. The smaller the
concentration of water in the external solution the greater the amount
of water that leaves the cell.

Prediction: I think that the higher the concentration of sucrose the
larger the amount of water will be transported into the potato cells
as osmosis is the net flow of water through a selectively permeable
membrane from a high water concentration to low water concentration.
This means the water molecules from a low sucrose solution will move
water molecules but not sugar molecules through the...

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